Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2024: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2023: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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Outline of Research at the Start |
We’ll construct full-length models of Aquifex and E. coli MotA/B as well as Vibrio PomA/B based on the recently determined structures of the transmembrane region of MotA/B and already known structure of the periplasmic domain of MotB together with all-atom molecular dynamics simulation. Using these models and PaCS-MD/MSM, we’ll simulate permeation of sodium ion and proton through the complex with and without peptidoglycan layer and also simulate the rotation of MotA or PomA around MotB or PomB. After that, we’ll compare the mechanisms of MotA/B and PomA/B.
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