Elucidating Pathogenesis Heterogeneity of Type III Secretion System at The Single-Cell Level of Human iPSC-Derived Organoid Model

• Project/Area Number: 23K14170
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 43060:System genome science-related
• Research Institution: Osaka University
• Principal Investigator: ALKADI MOHAMAD 大阪大学, 免疫学フロンティア研究センター, 特任研究員(常勤) (70963883)
• Project Period (FY): 2023-04-01 – 2025-03-31
• Project Status: Granted (Fiscal Year 2023)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2024: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2023: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: Organoids / Single-cell sequencing

Outline of Research at the Start

iPSC-derived organoids will be used to study the heterogeneity of different cell responses to Vibrio secretion system using single-cell RNA-seq. We will also address the coordination of different bacterial effectors to rewire the host pathways and validate the results experimentally.