| Project/Area Number |
23K15167
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 53020:Cardiology-related
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| Research Institution | Jichi Medical University |
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Principal Investigator |
メタタームタナチャイ 自治医科大学, 医学部, ポスト・ドクター (70910644)
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| Project Period (FY) |
2023-04-01 – 2025-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2024: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2023: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | Heart failure / TAC / Pressure overload / heart failure |
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| Outline of Research at the Start |
In this research, β-catenin and KLF5 inhibitors have benefits for preventing heart failure (HF) from pressure overload. Understanding the precise mechanism and target cell types is necessary to clarify the pathway of candidate compounds to develop effective drugs for preventing HF in the future.
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| Outline of Annual Research Achievements |
C57BL/6 male mice were subjected to transverse aortic constriction (TAC) to induce pressure overload. The rate of survival was monitored. Before being euthanized, the mice were subjected to echocardiography to assess ejection fraction (EF), wall thickness, and heart function. Then, after TAC surgery, the mice were subjected to echocardiography to assess the EF. The mice were euthanized and sampled to investigate every 1 week until 4 weeks after TAC. The heart failure-related gene expression was analyzed by qRT-PCR. Next, the expression of proteins in the up and downstream of each pathway was investigated. Some parts of metabolic alteration were measured by Mass spectrometry. The cell target in response to HF prevention will be continued to investigate.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
It takes time to prepare the sample from the TAC-mice model. Now, the tissues from the mice are available for the next step of the experiment.
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| Strategy for Future Research Activity |
The remaining gene and protein expressions will be performed by qRT-PCR, microarray, and Western blot. Flow cytometry will be used to measure cardiomyocytes, cardiac fibroblasts, and macrophages using immunolabeling or antibody. After identifying the cell target in response to HF prevention, gene expression will be validated and protein expression will be measured and confirmed using Mass spectrometry. Then, the whole metabolic alteration will be measured by Mass spectrometry.
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