| Project/Area Number |
23K15608
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 55060:Emergency medicine-related
|
|---|
| Research Institution | Hirosaki University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2023-04-01 – 2026-03-31
|
| Project Status |
Granted (Fiscal Year 2023)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2025: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2024: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2023: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
|---|
| Keywords | biodosimetry / dicentric / radiation / c-banding / cytogentic / centromere |
|---|
| Outline of Research at the Start |
Accurate and fast radiation dose estimation using cytogenetic biodosimetry is crucial in accidental and occupational exposures to ionizing radiation. To improve this, we've developed a new staining approach using solid stained dicentric chromosomes and a centromere highlighting technique.
|
| Outline of Annual Research Achievements |
We have combined the conventional solid chromosome staining with a specialized technique that targets and marks the centromeric region using a proteolytic enzyme, acetic saline, and Giemsa. We have reduced the staining to be completed within just ninety minutes. New modifications also minimize chromosome expansion while preserving morphology. This improvement enhanced the identification of chromosomal aberrations. Preliminary results show a significant reduction in dicentric yield measurement disparities among scorers, with the coefficient of variation decreasing from 12% with conventional Giemsa staining to 1.2%. We have begun to modify the technique for use in other laboratories, such as those in South Korea and Singapore. Results have also been shared at international conferences.
|
| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Our research is progressing smoothly in the laboratory. We have begun sharing our technique with other laboratories and at international conferences. However, we encountered an issue where some chemicals used in the experiment are available in Japan but not in other countries. To address this, we will collaborate with partner laboratories to modify the technique, ensuring it is accessible to all. We will also continue presenting our new staining method at international conferences and performing comparative analyses to validate its effectiveness.
|
| Strategy for Future Research Activity |
We plan to continue with manuscript preparation and publish our findings as soon as possible. Following publication, we will conduct intercomparison exercises using the new technique. These exercises will validate the method's effectiveness in reducing variation in chromosomal aberration scoring. By comparing results across different laboratories, we aim to demonstrate the consistency and reliability of our technique, further establishing its usefulness in the scientific community. Additionally, we will share this at a large international conference in the U.S., which will allow other laboratories to use the published results.
|
