| Project/Area Number |
23K16163
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 57060:Surgical dentistry-related
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
PAUDEL Durga 北海道医療大学, 先端研究推進センター, 助教 (20910590)
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| Project Period (FY) |
2023-04-01 – 2025-03-31
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| Project Status |
Discontinued (Fiscal Year 2023)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2025: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2024: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | microbiome / oral-gut-brain axis / brain / 16SrRNA sequencing / oral microbiome / gut microbiome / oral / gut-brain axis |
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| Outline of Research at the Start |
Oral and gut microbiomes are considered the most ecologically rich and taxonomically diverse microbiota communities. The gut microbiome is known to interact with the brain through the “gut-brain axis”. The possibility of the “oral-brain axis” in normal brain function will be explored in this study.
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| Outline of Annual Research Achievements |
.The gut microbiome and oral microbiome can communicate each other through their anatomical connection. Therefore, any alteration in the oral microbiota can affect the gut microbiota and vice-versa. Recently, for the first time, we showed that brain disorder such as psychological stress can affect the oral microbiome in an animal model.
Owing to the communication of oral and gut microbiome and their relation to brain, a three-way microbiota axis “oral-brain-gut” has been proposed. However, very limited studies have been done to determine the role of oral microbiome in the normal functioning of brain. Therefore, we aim to clarify the role of oral microbiome in maintaining normal brain function and the existence of microbiota oral-gut-brain axis using germ-free rodents.
We slightly changed our research plan. Initially, it was planned to study the effect of oral-gut-brain axis in normal brain function. However, it was important to understand how brain diseases such as Alzheimer's diseases could affect the oral and gut microbiome together. Therefore, in the first phase of the experiment, we collected oral swabs and gut stool from the Alzheimer's disease model mice. We are currently working on performing 16SrRNA sequencing of those samples. This will clarify if the oral-gut-brain axis affects the pathology in the brain such as in Alzheimer's disease.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Although we slightly deviated from the original research plan to achieve the research goal, the research is going smoothly. We have finished collecting oral swabs and stool samples from the Alzheimer's disease model mice. We have already extracted bacterial DNA from those samples and we are currently preparing a library for 16SrRNA sequencing of those samples.
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| Strategy for Future Research Activity |
Once we understand that brain diseases such as Alzheimer's disease are affected by oral microbiome and gut microbiome, we will start investigating if these microbiomes have a role in normal brain function as originally planned. We will use a germ-free mice model transplanted with oral and gut microbiome separately and together. We will study the normal brain function markers affected in each group. These data combined with the Alzheimer's mice model data will clarify the role of the microbiota oral-gut axis in normal and diseased brain conditions.
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