Zwitterionic Polymers-based Metal Phenolic Networks as Removable Protein Protectants
| Project/Area Number |
23K17211
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 90120:Biomaterials-related
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| Research Institution | Japan Advanced Institute of Science and Technology |
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Principal Investigator |
Rajan Robin 北陸先端科学技術大学院大学, 先端科学技術研究科, 助教 (70848043)
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| Project Period (FY) |
2023-04-01 – 2025-03-31
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| Project Status |
Discontinued (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2025: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2024: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2023: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | Metal Phenolic Network / Zwitterionic Polymers / Protein Stabilization / Magnetic Separation / Metal-phenolic network / core-shell nanoparticles / Protein aggregation / Biopharmaceutics / Surface coating |
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| Outline of Research at the Start |
To develop a novel multivalent phenolic system based on zwitterionic polymers for stimuli-responsive coatings, incorporating gold-iron oxide core-shell nanoparticles and PSPB to stabilize proteins under stress and create a removable protein stabilizer for off-the-shelf biopharmaceuticals.
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| Outline of Annual Research Achievements |
In the initial phase, I successfully developed and optimized a novel synthetic route for multivalent phenolic poly-sulfobetaine (MPPS), achieving the first-ever synthesis of this unique zwitterionic species. The process involved synthesizing multi-armed alkene and thiol compounds using thiol-ene click chemistry, establishing a robust precursor for further research. This work not only paves the way for groundbreaking applications in protein stabilization but also sets the stage for developing advanced biomaterials aimed at enhancing the stability and transport of therapeutic proteins. The successful synthesis of MPPS signifies a major step toward innovative biomedicine solutions, potentially transforming the storage and handling of protein-based pharmaceuticals.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The project has progressed smoothly, with significant achievements in developing a novel synthetic route for multivalent phenolic poly-sulfobetaine (MPPS). This effort involved synthesizing multi-armed alkene and thiol compounds via thiol-ene click chemistry, closely aligning with our planned objectives. Despite initial challenges in optimizing reaction conditions, effective adjustments were swiftly implemented, ensuring timely progress. This foundational success sets the stage for further exploration of protein stabilization applications with the synthesized MPPS, positioning us well for future research phases.
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| Strategy for Future Research Activity |
Having applied for the discontinuation of the Kakenhi grant, my focus is on ensuring a thorough conclusion of the research through appropriate channels. This entails overseeing the completion of remaining analyses and the synthesis of results into final reports and publications, to be handled by colleagues in Japan. The project's key accomplishment, the development of a novel synthetic route for multivalent phenolic poly-sulfobetaine (MPPS), will be documented comprehensively. My priority is to ensure that all research findings are meticulously recorded and made available to the scientific community, facilitating further studies in this innovative field.
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Report
(1 results)
Research Products
(2 results)
