| Project/Area Number |
23K19230
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0403:Biomedical engineering and related fields
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| Research Institution | Kawasaki Institute of Industrial Promotion Innovation Center of NanoMedicine |
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Principal Investigator |
Mohamed Aziz・Awaad・Aziz 公益財団法人川崎市産業振興財団(ナノ医療イノベーションセンター), ナノ医療イノベーションセンター, 研究員 (50978286)
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| Project Period (FY) |
2023-08-31 – 2025-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2024: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | polycarboxybetaine / immunogenicity / anti-tumor / drug delivery systems / pH-responsive |
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| Outline of Research at the Start |
For effective tumor targeting and smart drug delivery, the pH-responsive PGLu(DET-Car)-based polymeric micelles will be used for tumor therapy. The obtained data from this protocol will introduce detailed information about smart nanocarriers used for anti-tumor drug delivery systems development.
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| Outline of Annual Research Achievements |
Successfully were prepared a polyzwitterion termed as PGLu(DET-car) was prepared with a pH-responsive against tumorous pH and with lower immunogenicity compared with conventional polymers. The PGLu(DET-car) polymer showed lower induction of serum immunoglobulin (IgM, IgG) in vivo and cytokines (IL-2, IFN‐γ) induction in vitro compared with polyethylene glycol (PEG) polymer. Additionally, conjugation of PGLu(DET-car) into PEG polymer reduced the Anti-PEG IgM in the blood serum. The polymeric micelles prepared from the PGLu(DET-car) showed compatible affinity to reach deeper in the tumor site compared with those prepared from PEG. Furthermore, the PGLu(DET-car) micelles showed lower induction of IgM in the blood serum, as well as lower induction of IL-2, IFN‐γ cytokines in vitro.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
We successfully prepared a pH-responsive PGLu(DET-car)-based micelle. Additionally, we evaluated the immunogenicity of the prepared micelle both in vitro and in vivo. The current data showed lower immunogenicity of PGLu(DET-car) polymer as well as PGLu(DET-car)-based micelle compared with conventional polymers.
Unfortunately, we found difficulty loading a drug in the prepared micelles. The drug-loaded micelle preparation still needs more characterization and evaluation. we found unstable micelle after loading with anti-cancer drugs. therefore, it will take some time to optimize he conditions of loading drugs in the prepared micelles.
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| Strategy for Future Research Activity |
During the current year we will evaluate the in vivo biodistribution of PGLu(DET-car)-based micelle. Additionally, anti-cancer or anti-aging drugs loading into the prepared micelle will be optimized and evaluated using different animal models.
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