| Project/Area Number |
23K19447
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0802:Biomedical structure and function and related fields
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| Research Institution | University of Toyama |
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Principal Investigator |
Karim Mariam 富山大学, 学術研究部医学系, 特命助教 (00984577)
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| Project Period (FY) |
2023-08-31 – 2025-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2024: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Bone Formation / NAD+ metabolism / Bone Mineral density / Aging / Osteoporosis |
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| Outline of Research at the Start |
Nicotinamide adenine dinucleotide is a life essential coenzyme. NAD+ metabolism has significant role in age related pathologies. Osteoporosis is geriatric disorder with unknown cause in relation to NAD+ metabolism. The goal is to establish therapeutics for osteoporosis using NAD+ boosting therapies.
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| Outline of Annual Research Achievements |
NAD+ depleted mice model was used to study bones, in which Denovo and Preiss handler pathways of NAD biosynthesis are ablated. I found NAD+ depletion cause abnormal faulty bone growth. Upon morphometrics bone showed high bone resorptive cell known as osteoclast. MicroCT analysis these mice showed low Bone mineral density which is clinical sign for osteoporosis. Hence, it is established that NAD+ levels at young age is crucial for maintaining bone health. Research presented in following conferences:
1.Karim M, Nakagawa T. NAD+ level at a young age affects skeletal muscle functions at an old age. 日本トリフ-12442;トファン研究会 第42回学術集会; 2023 Dec 2-3; 富山.
2.Karim M, Nakagawa T. NAD+ level at a young age affects skeletal muscle functions at an old age.第97回日本薬理学会年会; 2023 Dec 14-16; Kobe.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
Mice models were studied, and new finding are indicative towards the more specific Bone cell specific deletion. To elucidate the role of osteoclast specific NAD depletion. More mice models are in making process consuming slightly more time.
Moreover, this study aims to study old mice of 24months, which also extended the study time period.
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| Strategy for Future Research Activity |
For this promising study next, I would like to examine bones of Osteoclast specific NAD+ depleted mice. Along with the bone I will check the interplay of lipid and amino acid metabolism in maintaining bone NAD+ homeostasis. Histo morphometric analysis of old age mice will be considered. The plan is to establish therapeutics for osteoporosis targeting NAD+ boosting therapies.
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