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Molecular basis of microbial symbiosis: systematic elucidation of glycan-mediated interactions between organisms in the deep-sea

Research Project

Project/Area Number 23K20307
Project/Area Number (Other) 20H03322 (2020-2023)
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeMulti-year Fund (2024)
Single-year Grants (2020-2023)
Section一般
Review Section Basic Section 45040:Ecology and environment-related
Research InstitutionKyoto University

Principal Investigator

Nakagawa Satoshi  京都大学, 農学研究科, 准教授 (70435832)

Project Period (FY) 2024-04-01 – 2025-03-31
Project Status Completed (Fiscal Year 2024)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2024: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2023: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2021: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2020: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords糖鎖 / 共生 / 深海底熱水活動域
Outline of Research at the Start

本研究は、深海底熱水活動域において強固な共生関係にある生物間の相互作用を、世界で初めて第三の分子生物学(=糖鎖生物学)の観点から体系化することを目指している。深海底熱水活動域は、多様な大型生物が特定の微生物と絶対的な共生関係にあり、生物間の相互作用・相互認識の研究にとって最適のフィールドである。また深海共生系は、共生と感染の分子機構の類似性を進化の時間軸を加味して研究する絶好のモデルであり、本申請研究は極めて先駆的・独創的かつ分子生物学・進化学・社会生物学・病理学・免疫学といった幅広い研究分野への波及効果が極めて大きい。

Outline of Final Research Achievements

This study primarily aimed to elucidate the mechanisms of interaction and mutual recognition between microorganisms and their hosts-either large organisms or other microbes-in symbiotic relationships in extreme environments such as deep-sea hydrothermal fields. Special emphasis was placed on glycan-associated molecules, which are known to play key roles in broad interspecies recognition. Through this research, we successfully identified several unique glycans and their associated molecules in microbial symbioses inhabiting such extreme environments, and obtained novel insights into their functional roles and evolutionary significances.

Academic Significance and Societal Importance of the Research Achievements

糖鎖は主にヒトの病理学や生理学の領域で重要な研究対象とされてきたが、海洋に生息する微生物の生態学的・進化学的研究においてはほとんど注目されてこなかった。本研究では、極限環境における異種生物間の分子レベルでの情報伝達手段を解明することを通じて、人類に広く感染する病原性微生物の起源や、真核生物の誕生過程の解明に向けた画期的な知見を得た。極限環境にみられる微生物共生系は、感染と共生に共通する分子メカニズムを、進化的視点から包括的に検討するための理想的な研究対象であり、本研究で得られた成果は極めて独創的であるとともに、進化生物学や病理学など多様な研究分野に大きな波及効果をもたらすものである。

Report

(6 results)
  • 2024 Annual Research Report   Final Research Report ( PDF )
  • 2023 Annual Research Report
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (31 results)

All 2024 2023 2022 2021 2020

All Journal Article (12 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 11 results,  Open Access: 5 results) Presentation (18 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Book (1 results)

  • [Journal Article] Characterization of protein glycosylation in an Asgard archaeon2024

    • Author(s)
      Nakagawa Satoshi、Imachi Hiroyuki、Shimamura Shigeru、Yanaka Saeko、Yagi Hirokazu、Yagi-Utsumi Maho、Sakai Hiroyuki、Kato Shingo、Ohkuma Moriya、Kato Koichi、Takai Ken
    • Journal Title

      BBA Advances

      Volume: 6 Pages: 100118-100118

    • DOI

      10.1016/j.bbadva.2024.100118

    • Related Report
      2024 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] <i>N</i> -linked protein glycosylation in <i>Nanobdellati</i> (formerly DPANN) archaea and their hosts2024

    • Author(s)
      Nakagawa Satoshi、Sakai Hiroyuki D.、Shimamura Shigeru、Takamatsu Yoshiki、Kato Shingo、Yagi Hirokazu、Yanaka Saeko、Yagi-Utsumi Maho、Kurosawa Norio、Ohkuma Moriya、Kato Koichi、Takai Ken
    • Journal Title

      Journal of Bacteriology

      Volume: 206 Issue: 9

    • DOI

      10.1128/jb.00205-24

    • Related Report
      2024 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Helicovermis profundi gen. nov., sp. nov., a novel mesophilic, asporogenous bacterium within the Clostridia isolated from a deep-sea hydrothermal vent chimney2024

    • Author(s)
      Urara Miyazaki, Daiki Mizutani, Yurina Hashimoto, Akihiro Tame, Shigeki Sawayama, Junichi Miyazaki, Ken Takai, Satoshi Nakagawa
    • Journal Title

      Antonie Van Leeuwenhoek.

      Volume: 117 Issue: 1 Pages: 1-10

    • DOI

      10.1007/s10482-023-01919-9

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Haliovirga abyssi gen. nov., sp. nov., a mesophilic fermentative bacterium isolated from the Iheya North hydrothermal field, and proposal of Haliovirgaceae fam. nov2023

    • Author(s)
      Urara Miyazaki, Akihiro Tame, Junichi Miyazaki, Ken Takai, Shigeki Sawayama, Masaaki Kitajima, Akihiro Okamoto, Satoshi Nakagawa
    • Journal Title

      Int J Syst Evol Microbiol.

      Volume: 73 Issue: 8

    • DOI

      10.1099/ijsem.0.006014

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pyrofollis japonicus gen. nov. sp. nov., a novel member of the family Pyrodictiaceae isolated from the Iheya North hydrothermal field2023

    • Author(s)
      Urara Miyazaki, Masaru Sanari, Akihiro Tame, Masaaki Kitajima, Akihiro Okamoto, Shigeki Sawayama, Junichi Miyazaki, Ken Takai, Satoshi Nakagawa
    • Journal Title

      Extremophiles.

      Volume: 27 Issue: 3 Pages: 1-8

    • DOI

      10.1007/s00792-023-01316-0

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Simple and Effective Method for Solid Medium Cultivation of Strictly Hydrogen- and Sulfur-oxidizing Chemolithoautotrophs Predominant in Deep-‍sea Hydrothermal Fields2023

    • Author(s)
      Muto Hisashi、Miyazaki Junichi、Sawayama Shigeki、Takai Ken、Nakagawa Satoshi
    • Journal Title

      Microbes and Environments

      Volume: 38 Issue: 6 Pages: n/a

    • DOI

      10.1264/jsme2.ME23072

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Physiological and comparative proteomic characterization of Desulfolithobacter dissulfuricans gen. nov., sp. nov., a novel mesophilic, sulfur-disproportionating chemolithoautotroph from a deep-sea hydrothermal vent2022

    • Author(s)
      Hashimoto Yurina、Shimamura Shigeru、Tame Akihiro、Sawayama Shigeki、Miyazaki Junichi、Takai Ken、Nakagawa Satoshi
    • Journal Title

      Frontiers in Microbiology

      Volume: 13

    • DOI

      10.3389/fmicb.2022.1042116

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Nitrosophilus kaiyonis sp. nov., a hydrogen-, sulfur- and thiosulfate-oxidizing chemolithoautotroph within “Campylobacteria” isolated from a deep-sea hydrothermal vent in the Mid-Okinawa Trough2022

    • Author(s)
      Fukazawa So、Mino Sayaka、Tsuchiya Jiro、Nakagawa Satoshi、Takai Ken、Sawabe Tomoo
    • Journal Title

      Archives of Microbiology

      Volume: 205 Issue: 1 Pages: 12-12

    • DOI

      10.1007/s00203-022-03350-6

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Desulfomarina profundi gen. nov., sp. nov., a novel mesophilic, hydrogen-oxidizing, sulphate-reducing chemolithoautotroph isolated from a deep-sea hydrothermal vent chimney2021

    • Author(s)
      Hashimoto Yurina、Tame Akihiro、Sawayama Shigeki、Miyazaki Junichi、Takai Ken、Nakagawa Satoshi
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 71 Issue: 11

    • DOI

      10.1099/ijsem.0.005083

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hydrogenimonas urashimensis sp. nov., a hydrogen-oxidizing chemolithoautotroph isolated from a deep-sea hydrothermal vent in the Southern Mariana Trough2021

    • Author(s)
      Mino Sayaka、Shiotani Taiki、Nakagawa Satoshi、Takai Ken、Sawabe Tomoo
    • Journal Title

      Systematic and Applied Microbiology

      Volume: 44 Issue: 1 Pages: 126170-126170

    • DOI

      10.1016/j.syapm.2020.126170

    • NAID

      120007187742

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biogeochemical Implications of N2O-Reducing Thermophilic Campylobacteria in Deep-Sea Vent Fields, and the Description of Nitratiruptor labii sp. nov.2020

    • Author(s)
      Fukushi Muneyuki、Mino Sayaka、Tanaka Hirohisa、Nakagawa Satoshi、Takai Ken、Sawabe Tomoo
    • Journal Title

      iScience

      Volume: 23 Issue: 9 Pages: 101462-101462

    • DOI

      10.1016/j.isci.2020.101462

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 深海微生物と我々の関わり2020

    • Author(s)
      中川聡
    • Journal Title

      野口研究所時報

      Volume: 63 Pages: 46-52

    • NAID

      40022399865

    • Related Report
      2020 Annual Research Report
  • [Presentation] 酸性温泉に由来する新規 Nanobdellati (DPANN アーキア)及び宿主アーキアのグライコプロテオミクス2024

    • Author(s)
      森田祥空
    • Organizer
      第 36 回日本 Archaea 研究会講演会
    • Related Report
      2024 Annual Research Report
  • [Presentation] Exploring the Microbial Communities of Shikine-jima Hot Springs through Metagenomics2024

    • Author(s)
      Masaru Sanari
    • Organizer
      第37回日本微生物生態学会大会
    • Related Report
      2024 Annual Research Report
  • [Presentation] ナノア ーキアおよび宿主ア ーキアのグライコプロテオーム2023

    • Author(s)
      高松芳基
    • Organizer
      日本Archaea研究会第35回講演会
    • Related Report
      2023 Annual Research Report
  • [Presentation] DPANNおよび宿主アーキアの経時的グライコプロテオミクス2023

    • Author(s)
      中川聡
    • Organizer
      第36回日本微生物生態学会大会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 酸性温泉からの新規DPANNアーキア培養株の確立2023

    • Author(s)
      酒井博之
    • Organizer
      第36回日本微生物生態学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Glycobiological interactions between DPANN and host Archaea2023

    • Author(s)
      Satoshi Nakagawa
    • Organizer
      第6回ExCELLSシンポジウム
    • Related Report
      2023 Annual Research Report
  • [Presentation] Physiological and Metabolic Characteristics of a Novel Sulfur-disproportionating Bacterium Isolated from a Deep-sea Hydrothermal Vent Chimney at the Suiyo Seamount, Japan2022

    • Author(s)
      Yurina Hashimoto
    • Organizer
      12th Asian Symposium on Microbial Ecology
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 常温性アンモニア酸化アーキアを対象とするグライコプロテオーム解析2022

    • Author(s)
      須山友貴
    • Organizer
      第 34 回日本 Archaea 研究会講演会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Physiological and proteomic characteristics of a novel sulfur-disproportionating bacterium isolated from a deep-sea hydrothermal vent2022

    • Author(s)
      Yurina Hashimoto
    • Organizer
      18th International Symposium on Microbial Ecology
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dual energy metabolism in endosymbionts of Alviniconcha vent snails2022

    • Author(s)
      Junichi Miyazaki
    • Organizer
      18th International Symposium on Microbial Ecology
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DPANNアーキアおよびその宿主・非宿主のグライコプロテオミクス2022

    • Author(s)
      中川聡
    • Organizer
      第35回日本微生物生態学会大会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 深海底熱水活動域に優占する化学合成独立栄養微生物の平板培養方法の検討2022

    • Author(s)
      武藤久
    • Organizer
      第35回日本微生物生態学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 伊豆・小笠原および中部沖縄トラフの深海底熱水活動域から分離したPyrodictiaceae科アーキアの性状解析2021

    • Author(s)
      宮崎うらら
    • Organizer
      日本Archaea研究会第33回講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 深海底熱水活動域由来イプシロンプロテオバクテリアの平板培地での培養方法の検討2021

    • Author(s)
      武藤久
    • Organizer
      極限環境生物学会2021年度(第22回)年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 深海微生物を彩るキテレツな糖鎖2020

    • Author(s)
      中川聡
    • Organizer
      第21回比較グライコーム研究会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 伊豆・小笠原および中部沖縄トラフの深海底熱水活動域における新規微生物群の分離と性状解析2020

    • Author(s)
      宮崎うらら
    • Organizer
      極限環境生物学会2020年度(第21回)年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 深海底熱水活動域から分離した新規硫酸還元菌の生理・生態学的性状2020

    • Author(s)
      橋本侑里南
    • Organizer
      極限環境生物学会2020年度(第21回)年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 水曜海山・深海熱水噴出孔からの新規硫酸還元菌の分離と性状解析2020

    • Author(s)
      橋本侑里南
    • Organizer
      日本地球化学会 第67回オンライン年会
    • Related Report
      2020 Annual Research Report
  • [Book] 遺伝学の百科事典2022

    • Author(s)
      日本遺伝学会
    • Total Pages
      690
    • Publisher
      丸善出版
    • ISBN
      9784621306604
    • Related Report
      2021 Annual Research Report

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Published: 2020-04-28   Modified: 2026-01-16  

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