| Project/Area Number |
23K21431
|
|---|
| Project/Area Number (Other) |
21H02894 (2021-2023)
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Multi-year Fund (2024)
Single-year Grants (2021-2023) |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 53010:Gastroenterology-related
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
Nagae Genta 東京大学, 先端科学技術研究センター, 特任准教授 (10587348)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
緑川 泰 国立研究開発法人国立精神・神経医療研究センター, 病院 総合外科部, 部長 (10292905)
上田 宏生 東京大学, 先端科学技術研究センター, 特任講師 (70821916)
|
|---|
| Project Period (FY) |
2024-04-01 – 2025-03-31
|
| Project Status |
Completed (Fiscal Year 2024)
|
| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2024: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2023: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2022: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2021: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
|
|---|
| Keywords | 肝癌 / RNA修飾 / 網羅的解析 / 網羅的分析 |
|---|
| Outline of Research at the Start |
継続課題のため、記入しない。
|
| Outline of Final Research Achievements |
In this project, we established a novel detection method that combines comprehensiveness, high accuracy, and high resolution through mathematical optimization using deep learning based on quantitative measurement data of mRNA methylation obtained by biochemical and physicochemical methods. By applying this detection system to RNA samples extracted from liver cancer cells, we clarified mRNA metabolism and regulation mechanisms and abnormalities occurring in cancer cells in terms of both transcriptional regulation and post-transcriptional modification.
We have improved the accuracy of the high-resolution modification identification method using direct RNA sequencing of m6A modification regions in liver cancer cell lines, and verified the accuracy of the method using control samples with IVT-amplified RNA synthesized using the modified bases as material, and confirmed an accuracy of over 80%.
|
| Academic Significance and Societal Importance of the Research Achievements |
RNA修飾そのものは古くから知られていたが、近年になってその生命現象における機能的役割が次々と明らかになってきた。一方で、現状においても個々の修飾分子に限られた研究が多く、網羅的検出法の解析精度も十分でないことから、制御システムとしての本質的な理解には至っていない。本研究の開発目標であるmRNA修飾の包括的検出法は、こうした状況を飛躍的に前進させることが期待され、また、がん細胞の分子生物学的異常の理解に新たな側面を加えるとともに、新規薬剤標的の探索をも期待される。
|
