| Project/Area Number |
23K21776
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| Project/Area Number (Other) |
21H03654 (2021-2023)
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Multi-year Fund (2024)
Single-year Grants (2021-2023) |
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| Section | 一般 |
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| Review Section |
Basic Section 64040:Social-ecological systems-related
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| Research Institution | Osaka Ohtani University |
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Principal Investigator |
Uchii Kimiko 大阪大谷大学, 薬学部, 准教授 (90469619)
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| Co-Investigator(Kenkyū-buntansha) |
見坂 武彦 摂南大学, 理工学部, 教授 (80397661)
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| Project Period (FY) |
2024-04-01 – 2025-03-31
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| Project Status |
Completed (Fiscal Year 2024)
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| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2024: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2023: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Keywords | 環境DNA / 核DNAマーカー / 長鎖配列解析 / 種内変異 / 長鎖塩基配列解析 |
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| Outline of Research at the Start |
本研究では、個体群遺伝構造・遺伝的多様性を評価する手法としての環境DNA分析の機能拡張を目的とし、核rRNAをマーカーとする個体群遺伝構造・遺伝的多様性解析法の確立を行う。同時に、ナノポアシーケンサーを用いた長鎖塩基配列解析法を環境DNA分析に適用し、従来の環境DNA分析では困難であった遺伝的多様性が低い集団の個体群遺伝構造・遺伝的多様性解析を実現することを目指す。
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| Outline of Final Research Achievements |
This study aimed to advance environmental DNA (eDNA) analysis by evaluating the utility of nuclear DNA markers developed in the ribosomal RNA (rRNA) gene region as alternatives to conventional mitochondrial DNA (mtDNA) markers. It also sought to establish a long-read sequencing approach, using a nanopore sequencer, to overcome the limitations of conventional short-read technologies in eDNA applications. Using an rRNA ITS1 marker, the genetic structure of hybrid carp populations was analyzed, demonstrating that combining nuclear and mitochondrial markers enables more accurate assessments of genetic structure. Furthermore, long-read sequencing targeting a ~1,500 bp region spanning from 18S to 28S of the rRNA gene showed potential as a useful tool for fish community profiling.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で得られた成果は、従来のmtDNAの短い配列の解析に依存した環境DNA分析の限界を克服し、より高解像度な分類群推定と個体群遺伝解析を可能とする新たな手法の確立への土台となるものである。核DNAマーカーや長鎖配列解析の導入により、環境DNAによる生物多様性評価の精度と応用範囲が飛躍的に向上し、保全生態学や生物資源管理に貢献することが期待される。
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