| Project/Area Number |
23K23941
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| Project/Area Number (Other) |
22H02678 (2022-2023)
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Multi-year Fund (2024)
Single-year Grants (2022-2023) |
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| Section | 一般 |
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| Review Section |
Basic Section 45020:Evolutionary biology-related
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
LAUDET Vincent 沖縄科学技術大学院大学, 海洋生態進化発生生物学ユニット, 教授 (20898423)
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| Co-Investigator(Kenkyū-buntansha) |
木下 政人 京都大学, 農学研究科, 准教授 (60263125)
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| Project Period (FY) |
2022-04-01 – 2026-03-31
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| Project Status |
Granted (Fiscal Year 2024)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2025: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2024: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2023: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2022: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Keywords | Pigmentation / Clownfish / Thyroid hormones / Ontogenesis / Metamorphosis / Thyroid Hormones / Cora Reef Fishes / Ontogeny / Thyroid Hormone |
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| Outline of Research at the Start |
We hypothesize that differences in pigmentation pattern ontogeny between Amphiprion ocellaris and A. percula are linked to variations in thyroid hormone (TH) signaling. We will document and compare pigmentation patterns, measure TH levels, and analyze gene expression related to TH signaling. This study aims to uncover the developmental and genetic mechanisms behind phenotypic diversity in these species, offering insights into their ecological and evolutionary significance.
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| Outline of Annual Research Achievements |
Our purpose is to test the hypothesis that difference existing in pigmentation pattern ontogeny between two different clownfish species (Amphiprion ocellaris and A. percula) are linked to differences in thyroid hormone (TH) signaling. We have determined the ontogenetic transcriptome of these two species plus an outgroup and generated Crispr/Cas9 mutants of a gene controlling TH formation
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Because of the delay in setting-up our husbandry the main acheivements of our research has been delayed but we have now an excellent dataset of developmental transcriptomic to analyse and we have generated a Crispr/Cas9 line that show germline transmission. We are therefore very confident that important results will be generated very soon.
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| Strategy for Future Research Activity |
The work is now going into two main direction
1° We have transcriptomic data of A. ocellaris, A. percula and Premnas biaculeatus from hatching until the end of trunk bar formation (more than 100 days) post hatching. We have published the first part of this analysis for A. ocellaris in Roux et al., Cell Reports 2023. We are currently working on the comparative transcriptomic analysis of the three species.
2° In collaboration with our co-I Masato Kinoshita we have developped a Crispr/Cas9 line in A. ocellaris with a mutated version of the deiodinase 2 gene. These fishes should have less TH and exhibit a phenotype similar to A. percula. We have now founder fish that grow and will be crossed to generate homozygotes.
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