| Project/Area Number |
23K25843
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| Project/Area Number (Other) |
23H01146 (2023)
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Multi-year Fund (2024)
Single-year Grants (2023) |
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| Section | 一般 |
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| Review Section |
Basic Section 13040:Biophysics, chemical physics and soft matter physics-related
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
PIGOLOTTI Simone 沖縄科学技術大学院大学, 生物複雑性ユニット, 教授 (30812193)
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| Project Period (FY) |
2023-04-01 – 2026-03-31
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| Project Status |
Granted (Fiscal Year 2024)
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| Budget Amount *help |
¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2025: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2024: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2023: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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| Keywords | DNA replication / replisome / bacterial cell cycle |
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| Outline of Research at the Start |
The theory we developed, combined with experiments, permits to shed light on the DNA replication program of a broad range of organisms. We will apply our method to a set of model microbial organisms in different conditions, to understand the strategies they implement to replicate their genomes.
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| Outline of Annual Research Achievements |
During this FY, we achieved two main goals of our project. The first goal has been to develop a general theory to describe the DNA abundance distribution from the genome replication dynamics. This general theory constitutes the basis of our approach. The paper based on this theory has been published in Plos Computational Biology. The second goal has been to startup our wet lab activity and successfully run the first benchmark experiments on E.coli. This success puts us in a very good position for completing the rest of the project.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The research is progressing as planned, with one paper already published and the groundwork for future experimental activity already done. We expect to soon make progress on applying our theory and experiment to novel systems, in particular to E.coli mutants and other microbial species, as detailed in the proposal of this project.
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| Strategy for Future Research Activity |
During this fiscal year, we expect to finalize our project on studying DNA replication in engineered E.coli strains with multiple origins of replication. Motivated by our recent theoretical results, we will also apply our method to budding yeast, to prove that our approach is not limited to prokaryotic species. We expect to obtain at least preliminary results on this second goal during this fiscal year.
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