Role of Dynein Heavy Chain 3`UTR in the axonal localization and translation of its mRNA

• Project/Area Number: 23K27107
• Project/Area Number (Other): 23H02414 (2023)
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Multi-year Fund (2024); Single-year Grants (2023)
• Section: 一般
• Review Section: Basic Section 43010:Molecular biology-related
• Research Institution: Okinawa Institute of Science and Technology Graduate University
• Principal Investigator: TERENZIO Marco 沖縄科学技術大学院大学, 分子神経科学ユニット, 准教授 (60867513)
• Project Period (FY): 2023-04-01 – 2026-03-31
• Project Status: Granted (Fiscal Year 2024)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2025: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2024: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2023: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: Axonal translation / Dynein Heavy Chain / mRNA / Axonal Injury / Sensory Neurons

Outline of Research at the Start

We aim to characterize Dynein mRNA axonal localization and translation in sensory neurons using a mouse in which the localizing element is deleted by CRISPR CAS9 technology. We will characterize these mice for defects in axonal regeneration both in vivo and in vitro.