Development of Reversible Double-Layer Polymer Modification Technology to Break the Safety/Efficacy Trade-Off of Delivering Enzyme

• Project/Area Number: 23K28429
• Project/Area Number (Other): 23H03740 (2023)
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Multi-year Fund (2024); Single-year Grants (2023)
• Section: 一般
• Review Section: Basic Section 90120:Biomaterials-related
• Research Institution: Kyushu University
• Principal Investigator: LI JUNJIE 九州大学, 先導物質化学研究所, 准教授 (80869892)
• Co-Investigator(Kenkyū-buntansha): 田中 賢 九州大学, 先導物質化学研究所, 教授 (00322850) ; 小林 慎吾 九州大学, 先導物質化学研究所, 特任准教授 (70625110)
• Project Period (FY): 2023-04-01 – 2026-03-31
• Project Status: Granted (Fiscal Year 2024)
• Budget Amount: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000); Fiscal Year 2025: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2024: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2023: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
• Keywords: enzyme delivery / polymer / oxidation therapy / polymer-enzyme conjugate / safety/efficacy / stimuli-responsiveness / nano-bio interaction

Outline of Research at the Start

This polymer delivery system can synchronize long circulation in blood, precise clearance and degradation by macrophage, and enzyme activation in tumor. The key points are to control hydrophilic-hydrophobic balance and number of modified polymers to enable (i) long blood circulation and macrophage-tropism together and (ii) degradation of enzyme before activation in macrophage.