Molecular mechanisms of pathogenesis induced by endophytic bacteria of Brassica species in a nutrient-dependent manner
| Project/Area Number |
23KF0025
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Multi-year Fund |
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| Section | 外国 |
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| Review Section |
Basic Section 39040:Plant protection science-related
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| Research Institution | The University of Tokyo |
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Principal Investigator |
晝間 敬 東京大学, 大学院総合文化研究科, 准教授 (20714504)
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| Co-Investigator(Kenkyū-buntansha) |
UTAMI YUNIAR 東京大学, 大学院総合文化研究科, 外国人特別研究員
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| Project Period (FY) |
2023-04-25 – 2025-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2024: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2023: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | 内生細菌 / 揮発性物質 / 栄養 / シロイヌナズナ / Pseudomonas |
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| Outline of Research at the Start |
植物に感染する微生物は同一個体であっても環境条件に応じて病原菌にもなったり、共生菌になったりする連続的な存在である。本研究は、植物の必須栄養素の一つでもあるリンの量に依存する形で植物に病原性を発揮する内生細菌のユニークな感染戦略を支える分子基盤に迫ることを目的としている。本研究により、栄養条件依存的に病原性を示す内生細菌の分子基盤が明らかになった暁にはその分子をターゲットとすることで、病原性発揮を特異的に抑止する方法論に繋がると期待している。
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| Outline of Annual Research Achievements |
In this project, we are trying to understand the potential roles of plant-associated bacteria isolated from healthy plants with root colonization by root endophyte Colletotrichum tofieldiae.
We discovered that one of the strains, specifically a Pseudomonas strain (Psv), can be detrimental to plants through the production of unidentified volatile compounds.
If Psv is grown in rich media such as LB media, the nearby plants will be wilted despite any no physical interaction, suggesting the production of harmful volatiles by Psv. However, Psv can produce beneficial volatiles for plants when grown on low-nutrient media such as 1/3 LB. From our lab collection, We also identified a strain of Pseudomonas aeruginosa (Pso) isolated from Arabidopsis thaliana that produces both harmful and beneficial volatiles, exhibiting a phenotypic effect similar to that of Psv. Importantly, we found that in Pso, the harmful volatile production under rich media cultivation can weaken with quorum sensing lasR gene abrogation. We are currently performing the whole genome sequencing for Psv.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
In the reconstitution test, we found that Psv was contaminated. We then re-purified it into a single isolate. However, pure Psv isolate direct inoculation to plants causes wilt regardless of the media phosphate status, which is contradictory to our previous hypothesis. Still, the volatile production by Psv signifies its ability to exert pathogenicity depending on nutrient status. Thus, we need to change some of our experimental settings during this first year. Further, we found that Psv's colony characteristics lead to difficulty in extracting high-quality DNA samples for whole genome sequencing so it takes longer than expected before.
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| Strategy for Future Research Activity |
After the whole genome sequencing is finished, we would like to do the genome and transcriptome comparison with Pso under different nutrient conditions that switch their capabilities from harmful volatile producers into beneficial ones. We also seek to see plant responses to those different volatiles by RNA sequencing and find genes with potential function in volatile sensing and response.
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Report
(1 results)
Research Products
(5 results)
