| Project/Area Number |
23KF0152
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Multi-year Fund |
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| Section | 外国 |
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| Review Section |
Basic Section 49050:Bacteriology-related
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| Research Institution | Osaka University |
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Principal Investigator |
飯田 哲也 大阪大学, 微生物病研究所, 教授 (90221746)
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| Co-Investigator(Kenkyū-buntansha) |
HASSAN JAYEDUL 大阪大学, 微生物病研究所, 外国人特別研究員
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| Project Period (FY) |
2023-09-27 – 2026-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2025: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2024: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2023: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | Providencia / T3SS / whole genome sequencing / mutant |
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| Outline of Research at the Start |
Type three secretion systems (T3SSs) are sophisticated nanomachinery used by a number of pathogenic microorganisms to exert their pathogenic effect. A genetically related T3SS was revealed on a plasmid carried by P. rustigianii. In this study, we will investigate the presence of T3SS genes in genus Providencia along with their location on the genome. Also, characterization of putative T3SSs effector proteins in Providencia spp. will be performed.
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| Outline of Annual Research Achievements |
The objective of the research was to examine the existence and locations of T3SS genes in Providencia, as well as to functionally characterize the injectosome. Initially, we screened Providencia isolates in our collection using PCR, followed by whole genome sequencing (WGS) to pinpoint the locations of T3SS genes. Through WGS, we identified the presence and precise locations of T3SS-related genes. Next, we are preparing mutants and complementary strains using various strategies for the functional analysis of the T3SS. Although no definitive conclusions can be drawn at this stage, we anticipate reporting interesting findings by the conclusion of this study.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The present study aimed to ascertain the presence of T3SS genes in Providencia. As intended, we examined several Providencia isolates and verified the presence of T3SS genes in some of them. To proceed with our next steps, we are currently preparing mutants and complementary strains to elucidate the functional traits of the T3SSs. At this juncture, we are optimistic about uncovering the role of T3SS in these Providencia strains and arriving at our final conclusions as scheduled. Hence, we anticipate that our work is advancing smoothly.
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| Strategy for Future Research Activity |
We're currently proceeding to step-2 of our research, involving a series of in vitro and in vivo experiments. We've selected specific genes and are now in the process of creating deletion mutants and constructing complementary strains. Our upcoming plan entails assessing the invasiveness and diarrheagenicity of the prepared strains through both in vitro and in vivo experiments. Once we've uncovered these traits, our goal shifts to understanding the role of T3SS genes in the interaction between Providencia strains (wild type, mutants, and complementary strains) and their host. To achieve this, we'll utilize live cell imaging and fluorescent microscopy techniques.
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