| Project/Area Number |
23KF0253
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Multi-year Fund |
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| Section | 外国 |
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| Review Section |
Basic Section 27040:Biofunction and bioprocess engineering-related
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| Research Institution | Osaka University |
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Principal Investigator |
永井 健治 大阪大学, 産業科学研究所, 教授 (20311350)
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| Co-Investigator(Kenkyū-buntansha) |
BOONS RANI 大阪大学, 産業科学研究所, 外国人特別研究員
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| Project Period (FY) |
2023-11-15 – 2026-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2025: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2024: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2023: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Bioluminescent plant / Callus cultivation / 3D bio-printing / 3D plant cell culture |
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| Outline of Research at the Start |
We envision to expand the DNA library available to produce gene constructs that i) can be triggered by specific physical or chemical inputs and that ii) produce a range of bioluminescent proteins that generate light at different wavelengths in plant cells.
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| Outline of Annual Research Achievements |
During this first stage, I acquired knowledge of genetically modifying plant cells. This led to the continuation of a collaborative research project based on fungal luciferase, and resulted in one new bioluminescent plant strain.
Regarding the 3D printing of plant cells, I chose widely available biopolymers, such as agarose and alginate as the main ink constituents. I produced some ink compositions based on microgels, which are not yet fully optimal and need further optimisation.
I also attempted first 3D printing tests with the bio-inks, containing plant callus cells. The latter has the tendency to form cell aggregates causing the nozzle to clog. This indicates the importance of optimising callus cultivation and collection for 3D printable bio-inks.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
As I have foreseen, the first months were used for my adaptation and learning gene editing techniques as well as gathering the necessary equipment or connecting with the right collaborators to use theirs, and adapting the protocols to the available equipment.
I have defined the platform requirements and have been conducting preliminary experiments to tackle the complications associated with 3D printing of plant cells. The results achieved so far have helped me gain a better understanding of the system and possible pitfalls, allowing me to redirect the next steps towards achieving the set goals.
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| Strategy for Future Research Activity |
Firstly, in the scope of the bioluminescent variants, my aim is to prepare a liquid callus culture from the newly obtained bioluminescent plant. Further, in collaboration, I aim to establish other colour variants to expand the available palette of bioluminescent plant callus.
Next, my objective is to establish a robust protocol for obtaining callus cells in sizes that allow use in high density plant cell 3D printing.
Finally, based on the results for the microgel-based agarose gels, I will conduct further tests towards the best composition for these 3D printing inks, including mechanical characterisation. Afterwards, I will consider the 3D design and post-printing conditions for optimal survival and bioluminescence emission.
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