| Project/Area Number |
23KF0286
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Multi-year Fund |
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| Section | 外国 |
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| Review Section |
Basic Section 46030:Function of nervous system-related
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
銅谷 賢治 沖縄科学技術大学院大学, 神経計算ユニット, 教授 (80188846)
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| Co-Investigator(Kenkyū-buntansha) |
GAMANUT ANDREI-RAZVAN 沖縄科学技術大学院大学, 神経計算ユニット, 外国人特別研究員
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| Project Period (FY) |
2023-11-15 – 2026-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2025: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2024: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2023: ¥200,000 (Direct Cost: ¥200,000)
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| Keywords | Claustrum / Retrosplenial cortex / Default Mode Network / Serotonin / Calcium imaging / Opto-genetics / Spiking neural networks |
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| Outline of Research at the Start |
The project will be subdivided in two parts, each with a specific aim: Aim 1 (experimental): reveal the response of claustrum neurons to serotonin, and the accompanying changes in behaviour; Aim 2 (computational): model how different types of serotonin receptors fine-tune the claustrum, and the effect of the tuning on the cortical dynamics.
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| Outline of Annual Research Achievements |
Experiments. Injections of retrograde viruses were successfully made in retrosplenial cortex, and the result was infection of neurons in claustrum. These injections tested the quantity of virus that induces the optimal expression of fluorescent proteins in the claustrum, which will be used for imaging of calcium activity. Also, tests were made to precisely implant the prism-GRIN probes from Inscopix next to the claustrum, and the success was confirmed with micro-CT scans of the brain.
Computational model. The interaction between claustrum and a simplified cortical region was simulated. The model consists of ~ 12,000 point neurons. The adaptive exponential integrate and fire neuronal model fitted the dynamics of the claustrum neurons, while the cortical neurons were of Hodgkin-Huxley type.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
There were no major events to interfere with the initial plan of research. The experiments can be continued as planned, with no major revisions. It is expected that the control of serotonin release in the claustrum can be achieved following the current experimental design. The computational model contains sufficient details to explore the dynamics of the interaction between claustrum and cortex and the influence of serotonin can be introduced in the model as planned.
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| Strategy for Future Research Activity |
Experimental. As originally planned, mice will be monitored during tasks, periods of awake rest and periods of sleep. Simultaneously, first the Calcium activity of the retrosplenial-projecting claustrum neurons will be imaged and their collective dynamics will be associated to each behavioural state. Then, the release of serotonin in the claustrum will be controlled with opto-genetics, and the impact on neuronal dynamics and on behaviour will be assessed.
Computational. First, simulations of the claustrum-cortex model will be done, each with a unique set of parameters, to explore the possible states of the system. Then, as planned, the effects of serotonin on the claustrum will be simulated by introducing equivalent currents of major serotonergic receptors in simulated claustrum neurons.
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