| Project/Area Number |
23KJ2167
|
|---|
| Research Category |
Grant-in-Aid for JSPS Fellows
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 国内 |
|---|
| Review Section |
Basic Section 90120:Biomaterials-related
|
|---|
| Research Institution | National Institute for Materials Science |
|---|
Principal Investigator |
王 洪欣 国立研究開発法人物質・材料研究機構, 高分子・バイオ材料研究センター, 特別研究員(RPD)
|
|---|
| Project Period (FY) |
2023-04-25 – 2026-03-31
|
| Project Status |
Granted (Fiscal Year 2023)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2025: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2024: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2023: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Keywords | intracellular force / stress / AFM indentation |
|---|
| Outline of Research at the Start |
90% of cancer deaths is due to metastasis. It is important to evaluate metastatic potential at an earlier stage. I plan to develop a new method based on prestress measurement on cytoskeleton. Unlike conventional cancer type-dependent diagnosis, my method can be generally applied to all cancer types.
|
| Outline of Annual Research Achievements |
We invented a unique way to‘touch’a cell with nanoscale‘hand’, so that the intracellular force (prestress) distribution, rather than commonly discussed modulus, over a complete cell could be mapped by atomic force microscopy (AFM). Such task was achieved by using an informatics-assisted AFM indentation technique to search for both the models used to fit indentation force-displacement curves and probe geometry descriptors. It enabled lateral prestress to be mapped with nanometric details.
Furthermore, we visualized dynamic mechanical responses of the cells adapting to environmental stimuli in situ. Our results highlight the alteration in the intracellular forces to attenuate environmental stimuli, such as rescue from mechanical stimulus-initiated cell death and initiation of cell migration.
|
| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
This result was not only published in STAM and selected as Editor's choice, but also received an excellent poster award at an academic conference.
In addition, AFM measurements were used in research to develop cell scaffolds based on ionic liquid interface, and the results were published in Adv. Mater.
|
| Strategy for Future Research Activity |
Following the demonstrated examples (Wang, etc. STAM, 2023) and power of this new characterization technique, future development should be further improving force sensitivity toward single molecule force measurement. It would enable direct understanding of mechanical roles for each molecular component in the cell response circuitry, when combining the technique with state-of-the art molecular imaging technologies.
|
