| Budget Amount *help |
¥222,430,000 (Direct Cost: ¥171,100,000、Indirect Cost: ¥51,330,000)
Fiscal Year 2016: ¥36,010,000 (Direct Cost: ¥27,700,000、Indirect Cost: ¥8,310,000)
Fiscal Year 2015: ¥36,400,000 (Direct Cost: ¥28,000,000、Indirect Cost: ¥8,400,000)
Fiscal Year 2014: ¥36,140,000 (Direct Cost: ¥27,800,000、Indirect Cost: ¥8,340,000)
Fiscal Year 2013: ¥36,140,000 (Direct Cost: ¥27,800,000、Indirect Cost: ¥8,340,000)
Fiscal Year 2012: ¥77,740,000 (Direct Cost: ¥59,800,000、Indirect Cost: ¥17,940,000)
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| Outline of Final Research Achievements |
Cytochrome P450BM3 (P450BM3) isolated from Bacillus megaterium catalyzes the hydroxylation of long-alkyl-chain fatty acids. We have demonstrated that even wild-type P450BM3 can catalyze the hydroxylation of gaseous alkanes such as ethane and propane as well as benzene by using perfluorinated carboxylic acids (PFCs) as decoy molecules. We also have demonstrated that N-perfluoroacyl amino acids strongly activate wild-type P450BM3 for the hydroxylation of inert alkanes. Furthermore, we showed that substrate-binding-state mimics of hydrogen peroxide-dependent cytochrome P450s prepared by one-point mutagenesis are able to catalyze monooxygenation of non-native substrates. The same mutation was also effective in introducing peroxygenase activity into P450BM3 and P450cam, indicating that a variety of peroxygenases based on P450s can be constructed by one-point mutagenesis.
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