| Project/Area Number |
24227002
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka City University |
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Principal Investigator |
Kamiya Nobuo 大阪市立大学, 複合先端研究機構, 教授 (60152865)
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| Co-Investigator(Kenkyū-buntansha) |
八ツ橋 知幸 大阪市立大学, 大学院理学研究科, 教授 (70305613)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAKAMI KEISUKE 大阪市立大学, 複合先端研究機構, 特任准教授 (40619904)
UMENA YASUFUMI 大阪市立大学, 複合先端研究機構, 特任准教授 (10468267)
Fukushima Yoshimasa 大阪市立大学, 複合先端研究機構, 特任講師 (00402438)
Noji Tomoyasu 大阪市立大学, 複合先端研究機構, 特任講師 (40452205)
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| Project Period (FY) |
2012-05-31 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥178,750,000 (Direct Cost: ¥137,500,000、Indirect Cost: ¥41,250,000)
Fiscal Year 2016: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2015: ¥28,990,000 (Direct Cost: ¥22,300,000、Indirect Cost: ¥6,690,000)
Fiscal Year 2013: ¥59,670,000 (Direct Cost: ¥45,900,000、Indirect Cost: ¥13,770,000)
Fiscal Year 2012: ¥63,440,000 (Direct Cost: ¥48,800,000、Indirect Cost: ¥14,640,000)
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| Keywords | 光化学系II / 光合成 / 水分解酸素発生機構 / X線結晶構造解析 / Mn4Caクラスター / Mn原子混合原子価 / Kokサイクル / 人工光合成 / 水分解 / 酸素発生 / 金属クラスター / 酸化状態 / SPring-8 / Ｘ線結晶構造解析 / 水分解酸素発生 / 酵素反応 |
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| Outline of Final Research Achievements |
We have reported the high-resolution structure of photosystem II (PSII), which is evolving oxygen from water molecules in photosynthesis, at 1.9 A in 2011. The structure involves the oxygen-evolving Mn-cluster at the most stable Kok’s S1 state. In order to understand the oxygen-evolving mechanism of PSII, activated structures of PSII should be resolved. In this study, we determined many structures of PSII crystals; four of complexes with herbicides, one of PsbM-subunit deleted mutant, two of ion-substituted derivatives from chloride to bromide or iodide, and so on. By studying intermediate states, S0 and S2, in the Kok cycle, new knowledge on the oxygen-evolving mechanism of PSII was obtained.
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| Assessment Rating |
Verification Result (Rating)
A
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Assessment Rating |
Result (Rating)
A: Progress in the research is steadily towards the initial goal. Expected research results are expected.
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