| Project/Area Number |
24227004
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (S)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
Nureki Osamu 東京大学, 大学院理学系研究科(理学部), 教授 (10272460)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
伊藤 耕一 東京大学, 新領域創成科学研究科, 教授 (10262073)
MATURANA ANDRES 名古屋大学, 生命農学研究科, 准教授 (10452004)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
Maturana Andrés D. 名古屋大学, 生命農学研究科, 准教授 (10452004)
|
|---|
| Project Period (FY) |
2012-05-31 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥217,880,000 (Direct Cost: ¥167,600,000、Indirect Cost: ¥50,280,000)
Fiscal Year 2016: ¥31,460,000 (Direct Cost: ¥24,200,000、Indirect Cost: ¥7,260,000)
Fiscal Year 2015: ¥31,720,000 (Direct Cost: ¥24,400,000、Indirect Cost: ¥7,320,000)
Fiscal Year 2014: ¥31,720,000 (Direct Cost: ¥24,400,000、Indirect Cost: ¥7,320,000)
Fiscal Year 2013: ¥46,020,000 (Direct Cost: ¥35,400,000、Indirect Cost: ¥10,620,000)
Fiscal Year 2012: ¥76,960,000 (Direct Cost: ¥59,200,000、Indirect Cost: ¥17,760,000)
|
|---|
| Keywords | X線結晶構造解析 / チャネル / トランスポーター / 遺伝学 / 電気生理学 / 膜輸送体 / リポソーム解析 / 計算機シミュレーション / 遺伝学解析 / パッチクランプ / 高分子構造・物性 / X線 / 生体分子 / タンパク質 / 薬理学 |
|---|
| Outline of Final Research Achievements |
Regarding ion channel, we solved high-resolution crystal structures of newly-identified Mg2+ channel MgtE, TRIC channel, P2X channel, and elucidated their substrate recognition mechanisms and gating mechanisms. For membrane transporters, we solved high-resolution crystal structures of Ca2+/H+ exchanger CAX, Fe2+ exporter FPN, sugar transporter SWEET, peptide transporter POT, amino acid exporter YddG, multi-drug exporter MATE, membrane protein integrator YidC to elucidate their substrate recognition mechanisms and transport mechanisms. We also solved high-resolution crystal structures of channel rhodopsin and light-driven Na+-pump KR2, which suggested how these rhodopsin channel/pump are gated upon light perception.
|
| Assessment Rating |
Verification Result (Rating)
A+
|
Assessment Rating |
Result (Rating)
A+: Progress in the research exceeds the initial goal. More than expected research results are expected.
|
