| Project/Area Number |
24227005
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Kanazawa University |
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Principal Investigator |
Ando Toshio 金沢大学, バイオAFM先端研究センター, 特任教授 (50184320)
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| Co-Investigator(Renkei-kenkyūsha) |
UCHIHASHI Takayuki 金沢大学, 数物科学系, 教授 (30326300)
FUKUMORI Yoshihiro 金沢大学, 自然システム系, 教授 (60135655)
FUKUMA Takeshi 金沢大学, 電子情報学系, 教授 (90452094)
KODERA Noriyuki 金沢大学, 理工研究域バイオAFM先端研究センター, 准教授 (30584635)
KONNO Hiroki 金沢大学, 理工研究域バイオAFM先端研究センター, 准教授 (80419267)
WONG Richard 金沢大学, 新学術創成研究機構, 教授 (30464035)
MURAKAMI Satoshi 東京工業大学, 生命理工学研究科, 教授 (30300966)
OGURA Teru 熊本大学, 発生医学研究所, 教授 (00158825)
TOYOSHIMA Yoko 東京大学, 総合文化研究科, 教授 (40158043)
KANDORI Hideki 名古屋工業大学, 工学研究科, 教授 (70202033)
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| Project Period (FY) |
2012-05-31 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥215,540,000 (Direct Cost: ¥165,800,000、Indirect Cost: ¥49,740,000)
Fiscal Year 2016: ¥30,290,000 (Direct Cost: ¥23,300,000、Indirect Cost: ¥6,990,000)
Fiscal Year 2015: ¥36,790,000 (Direct Cost: ¥28,300,000、Indirect Cost: ¥8,490,000)
Fiscal Year 2014: ¥45,500,000 (Direct Cost: ¥35,000,000、Indirect Cost: ¥10,500,000)
Fiscal Year 2013: ¥49,660,000 (Direct Cost: ¥38,200,000、Indirect Cost: ¥11,460,000)
Fiscal Year 2012: ¥53,300,000 (Direct Cost: ¥41,000,000、Indirect Cost: ¥12,300,000)
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| Keywords | 走査プローブ顕微鏡 / 1分子計測・操作 / バイオイメージング / タンパク質 / 細胞 / ダイナミクス / AFM / 走査型イオン伝導顕微鏡 / 構造生物 / １分子計測・操作 / 1分子計測 / 生物物理 / ナノバイオ / 生体分子 / 原子間力顕微鏡 / 高速AFM / イメージング / 細胞内オルガネラ / 機能動態 |
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| Outline of Final Research Achievements |
We worked on three subjects. In subject 1, we elucidated the functional mechanism of various protein systems by observing their dynamic processes using high-speed AFM (HS-AFM) that we had established before. Besides, we demonstrated that HS-AFM can analyze the structure of intrinsically disordered proteins that are difficult to analyze with conventional methods. In subject 2, we developed fast/wide-area scanning techniques without generation of unwanted vibrations and an interactive HS-AFM technique that can manipulate the sample during imaging. The usefulness of these new techniques were demonstrated. In addition, we developed cantilever-scan type of HS-AFM combined with fluorescence microscopy, and thereby, made it possible to capture HS-AFM and fluorescence images simultaneously. In subject 3, we developed high-speed scanning ion conductance microscopy that can image the sample without contact with the sample. The imaging speed reached a level 100-times faster than before.
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| Assessment Rating |
Verification Result (Rating)
A
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Assessment Rating |
Result (Rating)
A: Progress in the research is steadily towards the initial goal. Expected research results are expected.
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