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Elucidation of mechanisms of biomaterial conversion mediated by amino group-modifying carrier protein and application

Research Project

Project/Area Number 24228001
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionThe University of Tokyo

Principal Investigator

NISHIYAMA MAKOTO  東京大学, 生物生産工学研究センター, 教授 (00208240)

Co-Investigator(Kenkyū-buntansha) 富田 武郎  東京大学, 生物生産工学研究センター, 助教 (50447364)
Project Period (FY) 2012-05-31 – 2017-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥207,610,000 (Direct Cost: ¥159,700,000、Indirect Cost: ¥47,910,000)
Fiscal Year 2016: ¥41,210,000 (Direct Cost: ¥31,700,000、Indirect Cost: ¥9,510,000)
Fiscal Year 2015: ¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2014: ¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2013: ¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2012: ¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Keywordsキャリアタンパク質 / 生合成 / リジン生合成 / アルギニン生合成 / 二次代謝生合成 / 結晶構造解析 / 天然物化学 / 放線菌 / 二次代謝産物生合成 / 好熱菌 / アミノ酸生合成 / 進化 / 好熱性細菌 / 古細菌 / 結晶構造
Outline of Final Research Achievements

In this study, we found that lysine biosynthesis via a carrier protein (AmCP) that binds to the amino group we have previously found is widely distributed in the thermophilic archaea with evolutionarily old origins, and that it is also utilized as arginine biosynthesis system, suggesting that the system is the key to clarifying the biosynthesis evolution. We also succeeded in elucidating the crystal structures of complexes of various biosynthetic enzymes and AmCP, revealing that they recognize each other by electrostatic interaction for efficient reaction. Furthermore, we discovered that biosynthesis diverted from this system is also used for secondary metabolism of actinomycetes. All these results also clarified that the system plays an important role as a biosynthesis system.

Assessment Rating
Verification Result (Rating)

A+

Assessment Rating
Result (Rating)

A+: Progress in the research exceeds the initial goal. More than expected research results are expected.

Report

(9 results)
  • 2018 Research Progress Assessment (Verification Result) ( PDF )
  • 2017 Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report   Abstract(Research Progress Assessment) ( PDF )   Research Progress Assessment (Result) ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (74 results)

All 2017 2016 2015 2014 2013 2012 Other

All Int'l Joint Research (3 results) Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 6 results,  Acknowledgement Compliant: 4 results) Presentation (58 results) (of which Int'l Joint Research: 7 results,  Invited: 17 results) Book (1 results) Remarks (3 results)

  • [Int'l Joint Research] Max Plank 研究所(ドイツ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Max Planck Institute(Germany)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Scripps Institution of Oceanology(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Amino group carrier protein-mediated secondary metabolite biosynthesis in Streptomyces.2016

    • Author(s)
      Hasebe, F., Matsuda, K., Shiraishi, T., Futamura, Y., Nakano, T., Tomita, T., Ishigami, K., Taka, H., Mineki, R., Fujimura, T., Osada, H., Kuzuyama, T., Nishiyama, M.
    • Journal Title

      Nature Chemical Biology

      Volume: 12 Issue: 11 Pages: 967-972

    • DOI

      10.1038/nchembio.2181

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization of two β-decarboxylating dehydrogenases from Sulfolobus acidocaldarius2016

    • Author(s)
      Takahashi Kento、Nakanishi Fumika、Tomita Takeo、Akiyama Nagisa、Lassak Kerstin、Albers Sonja-Verena、Kuzuyama Tomohisa、Nishiyama Makoto
    • Journal Title

      Extremophiles

      Volume: 20 Issue: 6 Pages: 843-853

    • DOI

      10.1007/s00792-016-0872-4

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Lysine Biosynthesis ofThermococcus kodakarensiswith the Capacity to Function as an Ornithine Biosynthetic System2016

    • Author(s)
      Yoshida Ayako、Tomita Takeo、Atomi Haruyuki、Kuzuyama Tomohisa、Nishiyama Makoto
    • Journal Title

      Journal of Biological Chemistry

      Volume: 291 Issue: 41 Pages: 21630-21643

    • DOI

      10.1074/jbc.m116.743021

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Determinants of dual substrate specificity revealed by the crystal structure of homoisocitrate dehydrogenase from Thermus thermophilus in complex with homoisocitrate・Mg2+・NADH2016

    • Author(s)
      Takahashi Kento、Tomita Takeo、Kuzuyama Tomohisa、Nishiyama Makoto
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 478 Issue: 4 Pages: 1688-1693

    • DOI

      10.1016/j.bbrc.2016.09.004

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Crystal structure of the LysY・LysW complex from Thermus thermophilus2016

    • Author(s)
      Tetsu Shimizu, Takeo Tomita, Tomohisa Kuzuyama, and Makoto Nishiyama
    • Journal Title

      Journal of Biological Chemistry

      Volume: 291 Issue: 19 Pages: 9948-9959

    • DOI

      10.1074/jbc.m115.707034

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Structural insight into amino group-carrier protein-mediated lysine biosynthesis: crystal structure of the LysZ•LysW complex from Thermus thermophilus2015

    • Author(s)
      Ayako Yoshida, Takeo Tomita, Tsutomu Fujimura, Chiharu Nishiyama, Tomohisa Kuzuyama, and Makoto Nishiyama
    • Journal Title

      Journal of Biological Chemistry

      Volume: 290 Issue: 1 Pages: 435-447

    • DOI

      10.1074/jbc.m114.595983

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genome-wide comprehensive analysis of transcriptional regulation by ArgR in Thermus thermophilus2014

    • Author(s)
      Naoki Iwanaga, Kaori Ide, Takeshi Nagashima, Takeo Tomita, Yoshihiro Agari, Akeo Shinkai, Seiki Kuramitsu, Mariko Okada-Hatakeyema, Tomohisa Kuzuyama, and Makoto Nishiyama
    • Journal Title

      Extremophiles

      Volume: 18 Issue: 6 Pages: 995-1008

    • DOI

      10.1007/s00792-014-0669-2

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Two ATP-binding cassette transporters involved in (S)-2-aminoethyl-cysteine uptake in Thermus thermophilus2013

    • Author(s)
      Y. Kanemaru, F. Hasebe, T. Tomita, T. Kuzuyama, M. Nishiyama
    • Journal Title

      Journal of Bacteriology

      Volume: 195 Issue: 17 Pages: 3845-3853

    • DOI

      10.1128/jb.00202-13

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus.2013

    • Author(s)
      Ouchi T, Fujimura T, et al.
    • Journal Title

      Nat Chem Biol.

      Volume: 9(4):277-83 Issue: 4 Pages: 277-283

    • DOI

      10.1038/nchembio.1200

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Secondary metabolite biosynthesis mediated by amino-group carrier protein in Streptomyces2017

    • Author(s)
      NISHIYAMA, Makoto
    • Organizer
      9th US-Japan Seminar on the Biosynthesis of Natural Products
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Secondary metabolite biosynthesis mediated by amino-group carrier protein in Streptomyces2017

    • Author(s)
      NISHIYAMA, Makoto
    • Organizer
      Joint Symposium on Microbial Biotechnology
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Amino-group carrier protein mediated amino acid biosynthesis2017

    • Author(s)
      NISHIYAMA, Makoto
    • Organizer
      The 19th Japanese-German Workshop on Enzyme Technology
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 高度好熱菌Thermus thermophilus由来isopropylmalate synthaseのアシル化修飾による活性制御機構の解析2017

    • Author(s)
      吉田彩子、吉田稔、古園さおり、西山 真
    • Organizer
      日本農芸化学会2017年度大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新規アミノ酸DADHによるマスト細胞の活性抑制2017

    • Author(s)
      白井智丈、笠倉和巳、長谷部文人、松田研一、八代拓也、西山 真、西山千春
    • Organizer
      日本農芸化学会2017年度大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新規非タンパク性アミノ酸DADHによる免疫抑制作用2017

    • Author(s)
      関本崇弘、八代拓也、長谷部文人、松田研一、笠倉和巳、西山 真、西山千春
    • Organizer
      日本農芸化学会2017年度大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 好熱菌Thermus thermophilus由来のリジン生合成系酵素LysKとキャリアタンパク質LysWの相互作用機構に関する研究2017

    • Author(s)
      藤田理美、長谷部文人、CHO Su-Hee、富田武郎、葛山智久、西山 真
    • Organizer
      日本農芸化学会2017年度大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新規天然化合物s56-p1の特異なヒドラゾンユニットの生合成に関する研究2017

    • Author(s)
      松田研一、新家一男、葛山智久、西山 真
    • Organizer
      日本農芸化学会2017年度大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 超好熱・好酸性古細菌Sulfolobus acidocaldariusにおけるアルギニン生合成酵素遺伝子の転写調節機構に関する研究2017

    • Author(s)
      中西史佳、富田武郎、葛山智久、西山 真
    • Organizer
      日本農芸化学会2017年度大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Streptomyces sp. SpE090715-01 produces a maleimycin-like compound using amino-group carrier protein (AmCP)2017

    • Author(s)
      Muhammad Prima PUTRA, Naito KAZUHIRO, Matsuda KENICHI, Tomita TAKEO, Shin-ya KAZUO, Kuzuyama TOMOHISA, Nishiyama MAKOTO
    • Organizer
      日本農芸化学会2017年度大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Discovery and biosynthetic characterization of a natural product biosynthesized via AmCP-mediated pathway2017

    • Author(s)
      Kenichi Matsuda, Fumihito Hasebe, Yuh Shiwa, Yu Kanesaki, Takeo Tomita, Hirofumi Yoshikawa, Kazuo Shin-ya, Tomohisa Kuzuyama, and Makoto Nishiyama
    • Organizer
      Directing Biosynthesis V
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アミノ基キャリアタンパク質を介したリジン生合成酵素LysYの基質認識機構の構造基盤2016

    • Author(s)
      清水 哲、富田 武郎、葛山 智久、西山 真
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌)
    • Year and Date
      2016-03-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] アミノ酸キャリアタンパク質(AmCP)を介して生合成されるアミノ酸DADH修飾酵素の同定2016

    • Author(s)
      長谷部 文人、松田 研一、白石 太郎、富田 武郎、葛山 智久、西山 真
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] アミノ基キャリアタンパク質を指標とした新規天然化合物の探索及びその生合成に関する研究2016

    • Author(s)
      松田 研一、長谷部 文人、富田 武郎、志波 優、兼崎 友、吉川 博2、新家 一男、葛山 智久、西山 真
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] II型アミノ基キャリアタンパク質を介した二次代謝産物の生合成に関する研究2016

    • Author(s)
      内藤 一洋、松田 研一、長谷部 文人、富田 武郎、手塚 武揚、大西 康2、志波 優、吉川 博文、新家 一男、葛山 智久、西山 真
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] アミノ基キャリアタンパク質を介する生合成:発見と普遍性2016

    • Author(s)
      西山 真
    • Organizer
      東京理科大学 アグリ・バイオ公開シンポジウム
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] アミノ基結合型キャリアタンパク質を介したリジン生合成メタボロンの解析2016

    • Author(s)
      清水 哲、富田武郎、葛山智久、西山 真
    • Organizer
      生命システム原材料の起源と進化研究会
    • Related Report
      2016 Annual Research Report
  • [Presentation] アミノ酸生合成系における反応の共通性と普遍性2016

    • Author(s)
      西山 真
    • Organizer
      第89回 日本生化学会 フォーラム
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] アミノ基キャリアタンパク質を介した多様な化合物の生合成2016

    • Author(s)
      西山 真
    • Organizer
      静岡県立大学 食糧栄養科学セミナー
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] アミノ基結合型キャリアタンパク質を指標とした探索による新規天然化合物s56-p1発見とその生合成に関する研究2016

    • Author(s)
      松田研一、西山 真
    • Organizer
      第7回 醗酵学フォーラム
    • Related Report
      2016 Annual Research Report
  • [Presentation] アミノ基結合型キャリアタンパク質を介した二次代謝生合成機構の解明と新規有用生物活性物質の探索2016

    • Author(s)
      西山 真
    • Organizer
      公益財団法人 日本応用酵素協会 酵素研究助成 研究発表会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] アミノ酸代謝関連酵素の構造・機能・調節2016

    • Author(s)
      西山 真
    • Organizer
      浅野酵素活性分子プロジェクト研究成果報告会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] AmCP-mediated lysine biosynthesis and regulation2016

    • Author(s)
      Makoto Nishiyama
    • Organizer
      The Fifth International Symposium on Cofactors & Active Enzyme Molecule 2016
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] AmCP-mediated lysine biosynthesis and its regulation in thermophiles2016

    • Author(s)
      Makoto Nishiyama
    • Organizer
      The 11th International Congress on Extremophiles
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Analysis of protein acylation on enzymes involved in branched-chain amino acid in Thermus thermophilus2016

    • Author(s)
      Ayako Yoshida, Makoto Nishiyama, Minoru Yoshida, and Saori Kosono
    • Organizer
      The 11th International Congress on Extremophiles
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Structure and function of an ancestral-type β-decarboxylating dehydrogenase from Thermococcus kodakarensis2014

    • Author(s)
      Tetsu Shimizu, Lulu Yin, Kento Takahashi, Takeo Tomita, Tomohisa Kuzuyama, and Makoto Nishiyama
    • Organizer
      Active Enzyme Molecule 2014
    • Place of Presentation
      Toyama
    • Year and Date
      2014-12-17 – 2014-12-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 原始型β脱炭酸脱水素酵素の機能と構造基盤2014

    • Author(s)
      清水 哲、伊 録録、高橋賢人、富田武郎、葛山智久、西山 真
    • Organizer
      第5回醗酵学フォーラム
    • Place of Presentation
      湯河原
    • Year and Date
      2014-11-23 – 2014-11-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Amino acid-carrier protein-mediated biosynthesis of biomolecules2014

    • Author(s)
      Makoto Nishiyama
    • Organizer
      Japan-Italy Symposium
    • Place of Presentation
      Nara
    • Year and Date
      2014-11-05 – 2014-11-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Secondary metabolites biosynthesized using AmCP, a novel amino acid carrier protein, in Actinomycetes2014

    • Author(s)
      Kenichi Matsuda, Fumihito Hasebe, Takeo Tomita, Yu Shiwa, Hirofumi Yoshikawa, Kazuo Shin-ya, Tomohisa Kuzuyama, and Makoto Nishiyama
    • Organizer
      Japan-Italy Symposium
    • Place of Presentation
      Nara
    • Year and Date
      2014-11-05 – 2014-11-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] Thermococcus kodakarensis由来の祖先型β-脱炭酸脱水素酵素の機能と構造基盤の解析2014

    • Author(s)
      清水 哲、伊 録録、高橋賢人、富田武郎、葛山智久、西山 真
    • Organizer
      第15回極限環境生物学会
    • Place of Presentation
      沖縄
    • Year and Date
      2014-11-01 – 2014-11-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] 古細菌Sulfolobus acidocaldariusのアミノ酸生合成経路を構成する酵素の機能・構造解析2014

    • Author(s)
      富田武郎、高橋賢人、秋山 渚、西山 真
    • Organizer
      第15回極限環境生物学会
    • Place of Presentation
      沖縄
    • Year and Date
      2014-11-01 – 2014-11-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] Function and regulation of enzymes involved in amino acid biosynthesis of Sulfolobus acidocaldarius2014

    • Author(s)
      Takeo Tomita, Kento Takahashi, Nagisa Akiyama, and Makoto Nishiyama
    • Organizer
      第4回モデル生物丸ごと一匹学会
    • Place of Presentation
      大阪
    • Year and Date
      2014-09-26 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Characterization of an ancestral-type β-decarboxylating dehydrogenase from Thermococcus kodakarensis2014

    • Author(s)
      Tetsu Shimizu, Lulu Yin, Kento Takahashi, Takeo Tomita, Tomohisa Kuzuyama, and Makoto Nishiyama
    • Organizer
      第4回モデル生物丸ごと一匹学会
    • Place of Presentation
      大阪
    • Year and Date
      2014-09-26 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 多基質に活性を示すβ-脱炭酸脱水素酵素の構造基盤2014

    • Author(s)
      西山 真
    • Organizer
      第437回ビタミンB研究協議会
    • Place of Presentation
      山口
    • Year and Date
      2014-09-05 – 2014-09-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] アミノ基結合型キャリアタンパク質を指標とした新規天然化合物の探索2014

    • Author(s)
      松田研一、長谷部文人、富田武郎、志波 優、吉川博文、新家一男、葛山智久、西山 真
    • Organizer
      第49回天然物化学談話会
    • Place of Presentation
      倉敷
    • Year and Date
      2014-07-02 – 2014-07-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] アミノ酸キャリアタンパク質を介して 生合成される新規アミノ酸と その代謝産物の同定2014

    • Author(s)
      長谷部文人、富田武郎、高ひかり、藤村 務、西山千春、葛山智久、西山 真
    • Organizer
      第49回天然物化学談話会
    • Place of Presentation
      倉敷
    • Year and Date
      2014-07-02 – 2014-07-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] 放線菌におけるアミノ基キャリアタンパク質を介した二次代謝産物生合成2014

    • Author(s)
      西山 真
    • Organizer
      日本放線菌学会2014年度大会  基調講演
    • Place of Presentation
      筑波
    • Year and Date
      2014-06-19
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Screening of secondary metabolites biosynthesized with novel amino acid carrier protein system2014

    • Author(s)
      Kenichi Matsuda, Fumihito Hasebe, Takeo Tomita, Yu Shiwa, Hirofumi Yoshikawa, Kazuo Shin-ya, Tomohisa Kuzuyama, and Makoto Nishiyama
    • Organizer
      2014 ASBMB Annual Meeting
    • Place of Presentation
      San Diego
    • Year and Date
      2014-04-26 – 2014-04-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Lysine and arginine biosynthesis in thermococcus kodakarensis2013

    • Author(s)
      Ayako Yoshida, Makoto Nishiyama
    • Organizer
      Enzyme Technology XXII
    • Place of Presentation
      富山国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Carrier protein-mediated amino acid biosynthesis2013

    • Author(s)
      Makoto Nishiyama
    • Organizer
      17th German-Japanese Workshop on Enzyme Technology
    • Place of Presentation
      Technical University of Hamburg-Harburg
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] N-protecting protein LysW functions as a carrier protein in lysine biosynthesis on Thermus thremophilus2013

    • Author(s)
      Ayako Yoshida, Takeo Tomita, Saori Kosono, Tomohisa Kuzuyama, Makoto Nishiyama
    • Organizer
      17th German-Japanese Workshop on Enzyme Technology
    • Place of Presentation
      Technical University of Hamburg-Harburg
    • Related Report
      2013 Annual Research Report
  • [Presentation] Biosynthetic machinery of secondary metabolites using amino acid carrier protein in Streptomyces2013

    • Author(s)
      Fumihito Hasebe, Takeo Tomita, Tomohisa Kuzuyama, Makoto Nishiyama
    • Organizer
      17th German-Japanese Workshop on Enzyme Technology
    • Place of Presentation
      Technical University of Hamburg-Harburg
    • Related Report
      2013 Annual Research Report
  • [Presentation] Substrate recognition mechanism of a homolog of homoisocitrate dehydrogenase from Thermococcus kodakarensis, TK02802012

    • Author(s)
      Takeo Tomita
    • Organizer
      The 2nd Annual Meeting for Whole-Organism Science Society
    • Place of Presentation
      SPring-8(兵庫県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Analysis of structure-function relationships of homoisocitrate dehydrogenase homologs from Sulfolobus acidocaldarius2012

    • Author(s)
      Kento Takahashi
    • Organizer
      The 2nd Annual Meeting for Whole-Organism Science Society
    • Place of Presentation
      SPring-8(兵庫県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Functional analysis of lysine and arginine biosynthetic pathways in Thermococcus kodakarensis Yoshida Ayako, Tomita Takeo, Atomi Haruyuki, Kuzuyama Tomohisa, Nishiyama Makoto2012

    • Author(s)
      Ayako Yoshida
    • Organizer
      13th Swiss-Japanese Conference on Biotechnology and Bioprocess Engineering
    • Place of Presentation
      Walzenhausen, Switzerland
    • Related Report
      2012 Annual Research Report
  • [Presentation] アミノ酸キャリアタンパク質を介して生合成される新規アミノ酸とその代謝産物の同定

    • Author(s)
      長谷部文人、 富田武郎、高ひかり、藤村努、西山千春、葛山智久、西山真
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田校舎
    • Related Report
      2013 Annual Research Report
  • [Presentation] アミノ基結合型キャリアタンパク質を指標とした新規天然化合物の探索

    • Author(s)
      松田研一、長谷部文人、富田武郎、志波優、吉川博文、新家一男、葛山智久、西山真
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田校舎
    • Related Report
      2013 Annual Research Report
  • [Presentation] 超好熱・好酸性古細菌Sulfolobus acidocaldariusにおけるアミノ酸生合成経路の調節機構の解析

    • Author(s)
      秋山渚、富田武郎、葛山智久、西山真
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田校舎
    • Related Report
      2013 Annual Research Report
  • [Presentation] アミノ基修飾型キャリアタンパク質を介した二次代謝産物の生合成

    • Author(s)
      西山真
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田校舎
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Origin of lysine and arginine biosynthesis

    • Author(s)
      Makoto Nishiyama
    • Organizer
      Enzyme Technology XXII
    • Place of Presentation
      富山国際会議場
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] アーキアのリジン・アルギニン・ロイシン生合成酵素の構造・機能・進化

    • Author(s)
      西山 真
    • Organizer
      日本農芸化学会シンポジウム
    • Place of Presentation
      東北大学(仙台)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Thermococcus kodakarensisのリジン・アルギニン生合成酵素の基質認識機構の解明

    • Author(s)
      吉田彩子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東北大学(仙台)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 古細菌Sulfolobus acidocaldarius由来のホモイソクエン酸脱水素酵素ホモログの構造と機能の解析

    • Author(s)
      高橋賢人
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東北大学(仙台)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 超好熱・好酸性古細菌Sulfolobus acidocaldariusにおけるアミノ酸生合成酵素の転写調節機構の解析

    • Author(s)
      秋山渚
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東北大学(仙台)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 放線菌におけるアミノ酸キャリアタンパク質を介した二次代謝産物生合成

    • Author(s)
      長谷部文人
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東北大学(仙台)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 新規アミノ酸キャリアタンパク質を用いて生合成される天然化合物の探索

    • Author(s)
      松田研一
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東北大学(仙台)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Function and evolution of β-decarboxylating dehydrogenase family

    • Author(s)
      Lulu Yin
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東北大学(仙台)
    • Related Report
      2012 Annual Research Report
  • [Presentation] リジン生合成系の起源と進化

    • Author(s)
      西山 真
    • Organizer
      電気通信大学生命科学シンポジウム「生命システム原材料の起源と進化:生化学的禁制律」
    • Place of Presentation
      電気通信大学(東京)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] アミノ基結合型キャリアタンパク質を介した二次代謝生合成機構の解明と新規有用生物活性物質の探索2016

    • Author(s)
      長谷部文人、西山 真
    • Total Pages
      6
    • Publisher
      日本応用酵素協会
    • Related Report
      2016 Annual Research Report
  • [Remarks] 東京大学 生物生産工学研究センター 細胞機能工学研究室

    • URL

      http://park.itc.u-tokyo.ac.jp/biotec-res-ctr/saiboukinou/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 東京大学生物生産工学研究センター 細胞機能工学研究室

    • URL

      http://park.itc.u-tokyo.ac.jp/biotec-res-ctr/saiboukinou/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 東京大学生物生産工学研究センター 細胞機能工学研究室

    • URL

      http://park.itc.u-tokyo.ac.jp/biotec-res-ctr/saiboukinou/

    • Related Report
      2012 Annual Research Report

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Published: 2012-11-27   Modified: 2022-01-28  

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