Spatiotemporal and structural analysis of T cell activation regulation

• Project/Area Number: 24229004
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Saito Takashi 国立研究開発法人理化学研究所, 統合生命医科学研究センター, グループディレクター (50205655)
• Co-Investigator(Kenkyū-buntansha): 多根 彰子 (橋本彰子 / 多根 彰子(橋本彰子) / 橋本 彰子) 国立研究開発法人理化学研究所, 統合生命医科学研究センター, 上級研究員 (10415226)
• Project Period (FY): 2012-05-31 – 2017-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥218,010,000 (Direct Cost: ¥167,700,000、Indirect Cost: ¥50,310,000); Fiscal Year 2016: ¥43,290,000 (Direct Cost: ¥33,300,000、Indirect Cost: ¥9,990,000); Fiscal Year 2015: ¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000); Fiscal Year 2014: ¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000); Fiscal Year 2013: ¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000); Fiscal Year 2012: ¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
• Keywords: T細胞 / TCR-CD3複合体 / ミクロクラスター / シグナル伝達 / 細胞活性化 / イメージング解析 / 構造解析 / 免疫シナプス / 抗原認識 / T細胞活性化 / 立体構造解析

Outline of Final Research Achievements

T cell activation upon antigen recognition is found to be mediated by recruiting various signal molecules to TCR microclusters. The activation is regulated by several signals; positive and negative co-stimulation signals, innate signals, and cytoskeletal signals. We then analyzed spatiotemporal aspects of these regulations. Among them, we found the generation of inhibitory microclusters by inhibitory receptors PD-1 and new intracellular signaling sites for Ras activation. The structural basis of antigen recognition and activation through the TCR-CD3 complex has been tried to analyze by synthesis and structural analysis of whole molecules of the TCR-CD3 complex including intracellular domains. We have also clarified T cell activation status and their regulation of activation upon self-recognition by T cells in vivo.

Assessment Rating

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Research Products

• [Int'l Joint Research] Centre d'Immunologie de Marseille Luminy(France)
• [Int'l Joint Research] La Jolla Institute for Allergy & Immunol(米国)
• [Int'l Joint Research] University of Miami(米国)
• [Journal Article] Advax, a Delta Inulin Microparticle, Potentiates In-built Adjuvant Property of Co-administered Vaccines (2017)
  • Author(s): Hayashi Masayuki、Aoshi Taiki、Haseda Yasunari、Kobiyama Kouji、Wijaya Edward、Nakatsu Noriyuki、Igarashi Yoshinobu、Standley Daron M.、Yamada Hiroshi、Honda-Okubo Yoshikazu、Hara Hiromitsu、Saito Takashi、Takai Toshiyuki、Coban Cevayir、Petrovsky Nikolai、Ishii Ken J.
  • Journal Title: EBioMedicine Volume: 15 Pages: 127-136
  • DOI: 10.1016/j.ebiom.2016.11.015
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Analyzing the Dynamics of Signaling Microclusters (2017)
  • Author(s): Hashimoto-Tane A, Yokosuka T, Saito T.
  • Journal Title: The Immune Synapse Volume: 1584 Pages: 5-64
  • DOI: 10.1007/978-1-4939-6881-7_4
  • ISBN: 9781493968794, 9781493968817
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] CD4 CTL, a Cytotoxic Subset of CD4+ T Cells, Their Differentiation and Function (2017)
  • Author(s): Takeuchi Arata、Saito Takashi
  • Journal Title: Frontiers in Immunology Volume: 8 Pages: 194-194
  • DOI: 10.3389/fimmu.2017.00194
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Overexpression of Cytotoxic T-Lymphocyte–Associated Antigen-4 Prevents Atherosclerosis in Mice (2016)
  • Author(s): Takuya Matsumoto, Naoto Sasaki, Tomoya Yamashita, Takuo Emoto, Kazuyuki Kasahara, Taiji Mizoguchi, Tomohiro Hayashi, Keiko Yodoi, Naoki kitano, Takashi Saito, Tomoyuki Yamaguchi, and Ken-ichi Hirata
  • Journal Title: Arterioscler Thromb Vasc Biol. Volume: 36 Issue: 6 Pages: 1141-51
  • DOI: 10.1161/atvbaha.115.306848
  • Peer Reviewed / Open Access
• [Journal Article] CRTAM determines the CD4+ cytotoxic T lymphocyte lineage. (2016)
  • Author(s): Takeuchi, A., Badr Mel, S., Miyauchi, K., Ishihara, C., Onishi, R., Guo, Z., Sasaki. Y., Ike, H., Takumi, A., Tsuji, NM., Murakami, Y., Katakai, T., Kubo, M. and Saito, T.
  • Journal Title: J. Exp. Med. Volume: 213 Issue: 1 Pages: 123-138
  • DOI: 10.1084/jem.20150519
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Micro adhesion rings surrounding TCR microclusters are essential for T cell activation (2016)
  • Author(s): Akiko Hashimoto-Tane, Machie Sakuma, Hiroshi Ike, Tadashi Yokosuka, Yayoi Kimura, Osamu Ohara, Takashi Saito
  • Journal Title: Journal of Experimental Medicine Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Dynamic regulation of TCR-microcluster and microsynapse for T cell activation (2016)
  • Author(s): Hashimoto-Tane A., Yokosuka T. and Saito T.
  • Journal Title: Front. Immunol. Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification of Pathogenic Cardiac CD11c+ Macrophages in Nod1-Mediated Acute Coronary Arteritis. (2015)
  • Author(s): Motomura Y, et al.
  • Journal Title: Arterioscler Thromb Vasc Biol. Volume: 35 Issue: 6 Pages: 1423-33
  • DOI: 10.1161/atvbaha.114.304846
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Clustering of CARMA1 through SH3-GUK domain interactions is required for its activation of NF-kB signaling (2015)
  • Author(s): Hara, H., Yokosuka, T., Hirakawa, H., Ishihara, C., Yasukawa, S., Yamazaki, M., Koseki, H., Yoshida, H. and Saito, T.
  • Journal Title: Nat. Commun. Volume: 6 Issue: 1 Pages: 5555-5555
  • DOI: 10.1038/ncomms6555
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Autoantibodies to IgG/HLA class II complexes are associated with rheumatoid arthritis susceptibility (2014)
  • Author(s): Jin H, Arase N, Hirayasu K, Kohyama M, Suenaga T, Saito F, Tanimura K, Matsuoka S, Ebina K, Shi K, Toyama-Sorimachi N, Yasuda S, Horita T, Hiwa R, Takasugi K, Ohmura K, Yoshikawa H, Saito T, Atsumi T, Sasazuki T, Katayama I, Lanier LL, Arase H
  • Journal Title: Proceedings of National Academy of Science USA Volume: 111 Issue: 10 Pages: 3787-3792
  • DOI: 10.1073/pnas.1401105111
  • Peer Reviewed / Open Access
• [Journal Article] Quantitative proteomic analysis of signalsome dynamics in primary T cells identifies the CD6 surface receptor as a LAT-independent TCR signaling hub (2014)
  • Author(s): Roncagalli R., Hauri S., Fiore F., Liang Y., Chen Z., Sansoni A., Kanduri K., Joly R., Malzac A., Lahdesmaki H., Lahesmaa R., Yamasaki S., Saito T., Malissen M., Aebersold R., Gstaiger M., and Malissen B.
  • Journal Title: Nat. Immunol. Volume: 15 Issue: 4 Pages: 384-392
  • DOI: 10.1038/ni.2843
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Protein kinase C-η controls CTLA-4-mediated regulatory T cell function (2014)
  • Author(s): Kong KF, Fu G, Zhang Y, Yokosuka T, Casas J, Canonigo-Balancio AJ, Becart S, Kim G, Yates JR 3rd, Kronenberg M, Saito T, Gascoigne NR, and Altman A.
  • Journal Title: Nat. Immunol. Volume: 15 Issue: 5 Pages: 465-472
  • DOI: 10.1038/ni.2866
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Nucleic acid sensing by T cells initiates Th2 cell differentiation. (2014)
  • Author(s): Imanishi T, Ishihara C, Badr Mel S, Hashimoto-Tane A, Kimura Y, Kawai T, Takeuchi O, Ishii KJ, Taniguchi S, Noda T, Hirano H, Brombacher F, Barber GN, Akira S, Saito T
  • Journal Title: Nat Commun Volume: 10 Issue: 1 Pages: 3566-3566
  • DOI: 10.1038/ncomms4566
  • Peer Reviewed / Open Access
• [Journal Article] An ITAM-Syk-CARD9 signaling axis triggers contact hypersensitivity by stimulating IL-1 production in dendritic cells (2014)
  • Author(s): Yasukawa. S., Miyazaki, Y., Yoshii, C., Nakaya, M., Ozaki, N., Toda, S., Kuroda, E., Ishibashi, K., Yasuda, T., Natsuaki, Y., Mi-ichi, F., Iizasa, E., Nakahara, T., Yamazaki, M., Kabashima, K., Iwakura, Y., Takai, T., Saito, T., Kurosaki, T., Malissen, B., Ohno, N., Furue, M., Yoshida, H., and Hara, H
  • Journal Title: Nature Comm Volume: 5 Issue: 1 Pages: 3755-3755
  • DOI: 10.1038/ncomms4755
  • Peer Reviewed / Open Access
• [Journal Article] Transport of misfolded endoplasmic reticulum proteins to the cell surface by MHC class II molecules. (2013)
  • Author(s): Jiang, Y., Arase, N., Kohyama, M., Hirayasu, K., Suenaga, T., Jin, H., Matsumoto, M., Shida, K., L. Lanier, L., Saito, T. and Arase, H.
  • Journal Title: Int. Immunol. Volume: 25 Issue: 4 Pages: 235-246
  • DOI: 10.1093/intimm/dxs155
  • Peer Reviewed
• [Journal Article] Identification of distinct ligands for the C-type lectin receptors Mincle and Dectin-2 in the pathogenic fungus Malassezia. (2013)
  • Author(s): Ishikawa T, Itoh F, Yoshida S, Saijo S, Matsuzawa T, Gonoi T, Saito T, Okawa Y, Shibata N, Miyamoto T, Yamasaki S.
  • Journal Title: Cell Host Microbe. Volume: 13 Issue: 4 Pages: 477-488
  • DOI: 10.1016/j.chom.2013.03.008
  • Peer Reviewed
• [Journal Article] Construction of self-recognizing regulatory T cells from conventional T cells by controlling CTLA-4 and IL-2 expression (2013)
  • Author(s): Yamaguchi, T., Kishi, A., Osaki, M., Morikawa, H., Prieto-Martin, P., Wing, K., Saito, T., Sakaguchi, S.
  • Journal Title: Proc. Nat. Acad. Sci. USA. Volume: 110 Issue: 23
  • DOI: 10.1073/pnas.1307185110
  • Peer Reviewed
• [Journal Article] Bach2 maintains T cells in a naive state by suppressing effector memory-related genes (2013)
  • Author(s): Tsukumo, S., Unno, M., Muto, A., Takeuchi, A., Kometani, K., Kurosaki, T., Igarashi, K., Saito, T
  • Journal Title: Proc. Natl. Acad. Sci. U. S. A. Volume: 110 Issue: 26 Pages: 10735-40
  • DOI: 10.1073/pnas.1306691110
  • Peer Reviewed
• [Journal Article] Double-stranded RNA of small intestinal commensal but not pathogenic bacteria triggers TLR3-mediated IFN-β production by dendritic cells (2013)
  • Author(s): T. Kawashima, A. Kosaka, H. Yan, G. Zijin, R. Uchiyama, R. Fukui, D. Kaneko; Y. Kumagai; D.-J. You, J. Carreras, S. Uematsu, M. H. Jang, O. Takeuchi, T. Kaisho, S. Akira, K. Miyake, H. Tsutsui, T. Saito, I. Nishimura, N. M. Tsuji
  • Journal Title: Immunity Volume: 38 Issue: 6 Pages: 1187-1197
  • DOI: 10.1016/j.immuni.2013.02.024
  • Peer Reviewed
• [Journal Article] The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells (2013)
  • Author(s): Liang, Y., Cucchetti, M., Roncagalli, R., Yokosuka, T., Malzac, A., Bertosio, E., Imbert, J., Nijman, I.J., Suchanek, M., Saito, T., Wulfing, C., Malissen, B., Malissen, M.
  • Journal Title: Nat. Immunol. Volume: 14 Issue: 8 Pages: 858-866
  • DOI: 10.1038/ni.2634
  • Peer Reviewed
• [Journal Article] Membrane-bound human SCF/KL promotes in vivo human hematopoietic engraftment and myeloid differentiation. (2012)
  • Author(s): Takagi, S
  • Journal Title: Blood Volume: 119 Issue: 12 Pages: 2768-2777
  • DOI: 10.1182/blood-2011-05-353201
  • Peer Reviewed
• [Journal Article] Programmed cell death-1 forms negative costimulatorymicroclusters that directly inhibit T cell receptor signaling by recruiting phosphatase SHP2 (2012)
  • Author(s): Yokosuka T, Takamatsu M, Kobayashi-Imanishi W, Hashimoto-Tani A, Azuma M, Saito T
  • Journal Title: J Exp Med Volume: 209 Issue: 6 Pages: 935-945
  • DOI: 10.1084/jem.20112741
  • Peer Reviewed
• [Journal Article] Dynamism of immune reactions and regulation (2012)
  • Author(s): Kurosaki, T.
  • Journal Title: Int. Immunol. Volume: 24 Issue: 8 Pages: 473-475
  • DOI: 10.1093/intimm/dxs079
  • Peer Reviewed
• [Presentation] STING activation induces inhibition of cell growth and production of type-I IFN (2018)
  • Author(s): Takayuki Imanishi, Takashi Saito
  • Organizer: Keystone Symposia Conference T Cell Dysfunction, Cancer and Infection
  • Int'l Joint Research
• [Presentation] Dynamic regulation of T cell activation and co-stimulation at Immune synapse (2017)
  • Author(s): Takashi Saito
  • Organizer: Merck Special Seminar
  • Invited
• [Presentation] Regulation of T cell activation and function by innate signals -STING activation in T cells induce growth arrest and IFN production (2017)
  • Author(s): Takayuki Imanishi, Takashi Saito
  • Organizer: FASEB Summer Research conference: Signal Transduction in the Immune System
  • Int'l Joint Research
• [Presentation] Regulation of T cell activation and function by innate signals - STING activation induce growth arrest and IFN production in T cells - (2017)
  • Author(s): Takashi Saito
  • Organizer: 大阪大学 IFReC Colloquium
• [Presentation] Molecular assembly and function of PTPN22 in T cell receptor signaling (2017)
  • Author(s): Akiko Hashimoto-Tane, Takashi Saito
  • Organizer: 第46回日本免疫学会学術集会
• [Presentation] TCR-signals control STING-mediated type 1 IFN responses in T cells (2017)
  • Author(s): Takayuki Imanishi, Takashi Saito
  • Organizer: 第46回日本免疫学会学術集会
• [Presentation] TCR signaling introduces endocytic machinaries at the microclusters (2017)
  • Author(s): Hiroaki Machiyama, Kikumi Hata, Noriko Yanase, Akiko Hashimoto-Tane, Takashi Saito, Ei Wakamatsu, Tadashi Yokosuka
  • Organizer: 第46回日本免疫学会学術集会
• [Presentation] Adhesion-ring surrounding each TCR microcluster forms synapse-like structure essential for initial T cell activation (2016)
  • Author(s): Akiko Hashimoto-Tane, Machie Sakuma, Hiroshi Ike, Tadashi Yokosuka, Takashi Saito
  • Organizer: 第89回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-03-11
• [Presentation] Development and function of CD4+CTL in inflammation diseases (2016)
  • Author(s): Takashi Saito
  • Organizer: SICORP Japan-New Zealand Joint Research on Functional Foods
  • Place of Presentation: Wellington, New Zealand
  • Year and Date: 2016-02-12
  • Int'l Joint Research / Invited
• [Presentation] T cell-intrinsic role of the cytosolic DNAsensing for T cell function (2015)
  • Author(s): Takayuki Imanishi, Takashi Saito
  • Organizer: The 44th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-20
• [Presentation] The Inhibitory role of CIN85 adaptor protein in T cell functions (2015)
  • Author(s): Mei Suen Kong, Akiko Hashimoto-Tane, Tadashi Yokosuka, Tomohiro Kurosaki, Kia Kien Phua, Takashi Saito
  • Organizer: The 44th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-20
• [Presentation] The “Microsynapse” composed of a ring of focal adhesion molecules and a core of T cell receptor microclusters is an essential structure for T cell activation (2015)
  • Author(s): Akiko Hashimoto-Tane, Tadashi Yokosuka, Takashi Saito
  • Organizer: The 44th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-20
• [Presentation] Mechanism and status of T cell activation by self-recognition (2015)
  • Author(s): Mohamed Elsherif Badr, Takashi Saito
  • Organizer: The 44th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-20
• [Presentation] Engagement of TCR with foreign and self antigens trigger distinct signaling pathways to generate different T cell responses (2015)
  • Author(s): Moe Shiokawa, Eri Ishikawa, Masato Ogata, Takashi Saito, Sho Yomasaki
  • Organizer: The 44th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-20
• [Presentation] Cytoskeletal regulation of the clustering of a Ras-GEF, RasGRP1, upon T cell activation (2015)
  • Author(s): Tadashi Yokosuka, Akiko Hashimoto-Tane, Masae Furuhata, Hiroko Toyota, Takashi Saito
  • Organizer: The 44th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-20
• [Presentation] Search for substrates of Protein kinase D during thymocyte development (2015)
  • Author(s): Eri Ishikawa, Takashi Saito, Sho Yomasaki
  • Organizer: The 44th Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-19
• [Presentation] Regulation of T cell activation at Immune synapse (2015)
  • Author(s): Takashi Saito
  • Organizer: Advanced Seminar Series on Microbiology and Immunology
  • Place of Presentation: 大阪大学（大阪府大阪市）
  • Year and Date: 2015-11-10
  • Invited
• [Presentation] IL-2 signaling controls the sensitivity of TLR2 signaling in T cells (2015)
  • Author(s): Takayuki Imanishi, Takashi Saito
  • Organizer: TOLL CONFERENCE 2015
  • Place of Presentation: Marbella, Spain
  • Year and Date: 2015-09-30
  • Int'l Joint Research
• [Presentation] Mechanism and status of T cell activation by self-recognition (2015)
  • Author(s): Mohamed Elsherif Badr, Takashi Saito
  • Organizer: The 22nd IFReC Colloquium
  • Place of Presentation: 大阪大学（大阪府大阪市）
  • Year and Date: 2015-08-05
  • Invited
• [Presentation] T細胞を知る (2015)
  • Author(s): Takashi Saito
  • Organizer: 17th免疫サマースクール2015
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県淡路市）
  • Year and Date: 2015-07-21
• [Presentation] “Microsynapse” is an essential structure for T cell activation (2015)
  • Author(s): Takashi Saito
  • Organizer: FASEB Science Research Conference
  • Place of Presentation: Big Sky, Montana, USA
  • Year and Date: 2015-06-10
  • Int'l Joint Research
• [Presentation] The “Microsynapse” composed of a ring of focal adhesion molecules and a core of T cell receptor microclusters is an essential structure for T cell activation (2015)
  • Author(s): Akiko Hashimoto-Tane, Tadashi Yokosuka, Takashi Saito
  • Organizer: FASEB Science Research Conference
  • Place of Presentation: Big Sky, Montana, USA
  • Year and Date: 2015-06-07
  • Int'l Joint Research / Invited
• [Presentation] T細胞分化におけるPKDの基質および下流分子の同定 (2015)
  • Author(s): Eri Ishikawa, Takashi Saito, Sho Yomasaki
  • Organizer: 第25回Kyoto T Cell Conference (KTCC) 学術集会
  • Place of Presentation: 京都大学芝蘭会館（京都府京都市）
  • Year and Date: 2015-05-15
• [Presentation] The Inhibitory role of CIN85 adaptor protein in T cell functions (2015)
  • Author(s): Kong, M.S., Hashimoto-Tane A., Yokosuka, T., Kurosaki, T., and Saito, T.
  • Organizer: 第25回Kyoto T Cell Conference (KTCC) 学術集会
  • Place of Presentation: 京都大学芝蘭会館（京都府京都市）
  • Year and Date: 2015-05-15
• [Presentation] Mechanism and status of T cell activation by self-recognition (2015)
  • Author(s): Badr, M.E., and Saito T.
  • Organizer: 第25回Kyoto T Cell Conference (KTCC) 学術集会
  • Place of Presentation: 京都大学芝蘭会館（京都府京都市）
  • Year and Date: 2015-05-15
• [Presentation] Cytoskeletal regulation of the clustering of a Ras-GEF, RasGRP1, upon T cell activation (2015)
  • Author(s): Yokosuka, T., Hashimoto-Tane A., and Saito T.
  • Organizer: 第25回Kyoto T Cell Conference (KTCC) 学術集会
  • Place of Presentation: 京都大学芝蘭会館（京都府京都市）
  • Year and Date: 2015-05-15
• [Presentation] Spatiotemporal regulation of T cell activation (2015)
  • Author(s): Saito, T.
  • Organizer: OIST Seminar
  • Place of Presentation: 沖縄科学技術大学院大学（沖縄県那覇市）
  • Year and Date: 2015-04-07
• [Presentation] “Microsynapse” composed of focal adhesion molecules surrounding TCR microcluster is essential for T cell activation (2015)
  • Author(s): Akiko Hashimoto-Tane, Takashi Saito
  • Organizer: 2015 Keystone Symposia Conference
  • Place of Presentation: Snowbird, USA
  • Year and Date: 2015-03-30
• [Presentation] T細胞活性化開始点であるT細胞受容体ミクロクラスターを支える構造体ミクロシナプスの解析 (2015)
  • Author(s): Akiko Hashimoto-Tane, Takashi Saito
  • Organizer: 第88回日本薬理学会年会
  • Place of Presentation: 名古屋国際会議場（名古屋）
  • Year and Date: 2015-03-19
• [Presentation] Spacial regulation of T cell activation at Immune synapse (2015)
  • Author(s): Takashi Saito
  • Organizer: The Fourth Bizan Immunology Symposium at University of Tokushima
  • Place of Presentation: 徳島大学（徳島）
  • Year and Date: 2015-01-29
  • Invited
• [Presentation] IL-2 signaling controls the sensitivity of TLR2-medaited T cell activation (2014)
  • Author(s): Takayuki Imanishi, Toshitada Takemori, Shizou Akira, Takashi Saito
  • Organizer: The 43rd Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 国立京都国際会館（京都）
  • Year and Date: 2014-12-12
• [Presentation] Interferon-β induced by double-stranded RNA of lactic acid bacteria promotes differentiation of IFN-γ- producing Th1 cells (2014)
  • Author(s): Tadaomi Kawashima, Yohei Watanabe, Ryutaro Fukui,Takash Saitoi, Kensuke Miyake, Hiroko Tsutsui, Noriko M Tsuji
  • Organizer: The 43rd Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 国立京都国際会館（京都）
  • Year and Date: 2014-12-12
• [Presentation] Subcellular imaging of the E3 ubiquitin ligases, c-Cbl and Cbl-b, to control T cell activation signals through ubiquitin clustering at TCR microclusters (2014)
  • Author(s): Tadashi Yokosuka, Akiko Hashimoto-Tane, Takashi Saito,
  • Organizer: The 43rd Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 国立京都国際会館（京都）
  • Year and Date: 2014-12-11
• [Presentation] “Microsynapse” structure supporting TCR microclusters augments T cell activation (2014)
  • Author(s): Akiko Hashimoto-Tane, Tadashi Yokosuka, Takashi Saito
  • Organizer: The 43rd Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 国立京都国際会館（京都）
  • Year and Date: 2014-12-11
• [Presentation] Differential requirement of Erk1/2 in mature T cell responses induced by self and foreign antigens (2014)
  • Author(s): Moe Shiokawa, Eri Ishikawa, Masato Ogata, Takashi Saito, Sho Yamasaki
  • Organizer: The 43rd Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 国立京都国際会館（京都）
  • Year and Date: 2014-12-11
• [Presentation] Involvement of PAF metabolism in anaphylaxis and prevention by Rush-SOTI (2014)
  • Author(s): Noriko M Tsuji, Takao Shimizu, Takashi Saito, Zijin Guo
  • Organizer: The 43rd Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 国立京都国際会館（京都）
  • Year and Date: 2014-12-11
• [Presentation] CRTAM determines the CD4+ cytotoxic T lymphocyte lineage (2014)
  • Author(s): Arata Takeuchi, Reiko Onishi, Hiroshi Ike, Takashi Saito
  • Organizer: The 43rd Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 国立京都国際会館（京都）
  • Year and Date: 2014-12-11
• [Presentation] Identification of pro-IL-16 as a substrate of protein kinase D during T cell development (2014)
  • Author(s): Eri Ishikawa, Takash Saitoi, Sho Yamasaki
  • Organizer: The 43rd Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 国立京都国際会館（京都）
  • Year and Date: 2014-12-10
• [Presentation] Microsynapse structure regulating T cell activation (2014)
  • Author(s): Takashi Saito
  • Organizer: France - Japan Immunology meeting
  • Place of Presentation: Cassis, France
  • Year and Date: 2014-10-23
• [Presentation] Regulation of initial T cell activation and functional differentiation (2014)
  • Author(s): Takashi Saito
  • Organizer: Innovation summit Tokyo 2014
  • Place of Presentation: 東京大学医科学研究所（東京）
  • Year and Date: 2014-10-02
  • Invited
• [Presentation] T cell activation and differentiation upon direct sensing of nucleic acids (2014)
  • Author(s): Takashi Saito
  • Organizer: Cold Spring Harbor Asia Conferences
  • Place of Presentation: Suzhou, China
  • Year and Date: 2014-09-03
  • Invited
• [Presentation] T細胞活性化のダイナミック制御 (2014)
  • Author(s): Takashi Saito
  • Organizer: 免疫サマースクール2014
  • Place of Presentation: オリビアン小豆島（香川）
  • Year and Date: 2014-07-30
  • Invited
• [Presentation] Imaging of lymphocyte activation (2014)
  • Author(s): Takashi Saito
  • Organizer: The 37th Naito Conference
  • Place of Presentation: ヒルトンニセコビレッジ（北海道）
  • Year and Date: 2014-07-17
  • Invited
• [Presentation] Direct sensing of nucleotides by T cells induces Th2 differentiation (2014)
  • Author(s): Takashi Saito
  • Organizer: FASEB Science Research Conference
  • Place of Presentation: Snowmass, USA
  • Year and Date: 2014-06-30
  • Invited
• [Presentation] T細胞の活性化と副刺激の制御機構 ―がん免疫療法の標的分子の制御基盤― (2014)
  • Author(s): Takashi Saito
  • Organizer: 免疫シンポジウム2014
  • Place of Presentation: グランソール奈良（奈良）
  • Year and Date: 2014-06-21
  • Invited
• [Presentation] T細胞の活性化に関する基礎的研究について (2014)
  • Author(s): Takashi Saito
  • Organizer: 第4回T-cell Camp
  • Place of Presentation: ヒルトン小田原（小田原）
  • Year and Date: 2014-05-31
  • Invited
• [Presentation] Nucleic acids sensing by T cells induces co-stimulation to initiate Th2 cell differentiation (2014)
  • Author(s): Takashi Saito
  • Organizer: EMBO Conference
  • Place of Presentation: Bertinoro, Italy
  • Year and Date: 2014-05-20
  • Invited
• [Presentation] Distinct signalosome formation by two Ras-GEFs to activate the Ras-MAPK pathway in T cell signaling (2014)
  • Author(s): Tadashi Yokosuka, Akiko Hashimoto-Tane, Takashi Saito
  • Organizer: 第24回 Kyoto T Cell Conference
  • Place of Presentation: 京都大学（京都）
  • Year and Date: 2014-05-17
• [Presentation] 末梢T細胞のメンテナンスと活性化におけるシグナル伝達機構の違い (2014)
  • Author(s): 塩川 萌、石川 絵理、緒方 正人、斉藤 隆、山崎 晶
  • Organizer: 第24回 Kyoto T Cell Conference
  • Place of Presentation: 京都大学（京都）
  • Year and Date: 2014-05-17
• [Presentation] Dynamic regulation and modulation of T cell activation (2013)
  • Author(s): Saito T.
  • Organizer: Immunology 2013, AAI Annual Meeting
  • Place of Presentation: Honolulu, USA
  • Invited
• [Presentation] Direct activation of T cells by nucleic acids induces Th2 differentiation (2013)
  • Author(s): Saito T.
  • Organizer: Kyoto T Cell Conference
  • Place of Presentation: 京都大学（京都）
• [Presentation] Protein kinase D is a critical component for T cell development (2013)
  • Author(s): Ishikawa E., Saito T., Yamasaki S.
  • Organizer: Kyoto T Cell Conference
  • Place of Presentation: 京都大学（京都）
• [Presentation] Beta-glucan in diet induces IL-10-producing regulatory CD4+ T cells (Tr-1-like cells) in Peyer's patches and pretects mice from experimental colitis (2013)
  • Author(s): Yan H., Ohno N., Watanabe Y., Saito T., Guo Z., Takeuchi A., Tsuji N.
  • Organizer: Kyoto T Cell Conference
  • Place of Presentation: 京都大学（京都）
• [Presentation] Evidence for distinct TCR signaling towards maintenance and activation of peripheral T cells (2013)
  • Author(s): Shiokawa M., Ishikawa E., Ogata M., Saito T., Yamasaki S.
  • Organizer: Kyoto T Cell Conference
  • Place of Presentation: 京都大学（京都）
• [Presentation] 免疫応答の始まり -T細胞活性化のダイナミックな制御- (2013)
  • Author(s): 齊藤 隆
  • Organizer: 免疫サマースクール2013
  • Place of Presentation: ザ・ルイガンズ（博多）
• [Presentation] Molecular dynamics for T cell activation and costimulation (2013)
  • Author(s): Saito T.
  • Organizer: Frontiers in Immunology Conference
  • Place of Presentation: ソラシティカンファレンス センター（東京）
  • Invited
• [Presentation] Nucleic acid-mediated T cell activation through a novel sensing mechanism induces Th2 responses (2013)
  • Author(s): Imanishi T., Akira S., Saito T.
  • Organizer: ICI 2013
  • Place of Presentation: Milano, Italy
• [Presentation] Basilic, a lymphoid-specific protein essential for CD28 costimulation and regulatory T cell development (2013)
  • Author(s): Malissen M., Yinming L., Cucchetti M., Roncagalli R., Yokosuka T., Saito T., Wuelfing C., Malissen B.
  • Organizer: ICI 2013
  • Place of Presentation: Milano, Italy
• [Presentation] Double-stranded RNA of intestinal commensal but not pathogenic bacteria triggers production of protective IFN-β (2013)
  • Author(s): Tsuji N., Kawashima T., Yan H., Guo Z., Carreras J., Uchiyama R., Fukui R., Kaisho T., Akira S., Miyake K., Tsutsui H., Saito T.
  • Organizer: ICI 2013
  • Place of Presentation: Milano, Italy
• [Presentation] Dynamic regulation of T cell activation and co-sitmulation (2013)
  • Author(s): Saito T.
  • Organizer: 4th International Symposium on Regulators of Adaptive Immunity
  • Place of Presentation: Erlangen, Germany
  • Invited
• [Presentation] Dynamic regulation of T cell activation and co-stimlation (2013)
  • Author(s): Saito T.
  • Organizer: 43rd Annual Scientific Meeting Australasian Society for Immunology (ASI ASM 2013)
  • Place of Presentation: Wellington, New Zealand
  • Invited
• [Presentation] Nucleid acids sensing by T cells initiates Th2 cell differentiation (2013)
  • Author(s): Saito T.
  • Organizer: Germany-Japan Immunology Seminar 2013
  • Place of Presentation: 日本平ホテル（静岡）
  • Invited
• [Presentation] Distinct signalosome formation by two Ras-GEFs to activate the Ras-MAPK pathway in T cell signaling (2013)
  • Author(s): Yokosuka, T., Hashimoto-Tane, A., Saito, T.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ（千葉）
• [Presentation] Innate adaptor molecule, IPS-1 is involved in regulating production of IL-17 and IL-22 from Th17 cells (2013)
  • Author(s): Onishi R., Takeuchi A., Kong MS., Imanishi T., Kawai T., Akira S., Saito T.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ（千葉）
• [Presentation] Direct recognition of nucleic acids by T cells initiates Th2 cell differentiation (2013)
  • Author(s): Imanishi, T., Akira, S., Saito T.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ（千葉）
• [Presentation] Arp2/3 complex-mediated actin-nucleation supports T cell receptor microcluster (2013)
  • Author(s): Hashimoto-Tane, A., Yokosuka, T., Saito, T.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ（千葉）
• [Presentation] CRTAM+CD4+T cells represent a lineage of CD4+ cells with CTL function (2013)
  • Author(s): Takeuchi A., Saito, T.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ（千葉）
• [Presentation] Search for downstream targets of Protein kinase D that is critical for T cell development (2013)
  • Author(s): Ishikawa E., Ohora M., Kurosaki T., Saito T., Yamasaki S.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ（千葉）
• [Presentation] Double-stranded RNA of intestinal commensal but not pathogenic bacteria triggers production of protective IFN-β (2013)
  • Author(s): Kawashima T., Yan H., Guo Z., Carreras J., Uchiyama R., Fukui R., Kaisho T., Akira S., Miyake K., Tsutsui H., Saito T., Tsuji N.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ（千葉）
• [Presentation] Evidence for distinct TCR signaling towards maintenance and activation of peripheral T cells (2013)
  • Author(s): Shiokawa M., Ishikawa E., Ogata M., Saito T., Yamasaki S.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ（千葉）
• [Presentation] Rheumatoid factor binding to IgG heavy chain presented on HLA-DR is associated with Rheumatoid Arthritis susceptibility (2013)
  • Author(s): Jin H., Arase N., Kohyama M., Saito F., Hirayasu K., Matsumoto M., Shida K., Suenaga T., Saito T.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ（千葉）
• [Presentation] Dynamic and cytoskeletal regulation of T cell activation (2013)
  • Author(s): Saito, T.
  • Organizer: Riken Center for Allergy and Immunology:University of Michigan Medical Center Joint Workshop
  • Place of Presentation: Detroit, USA
• [Presentation] Dynamic imaging analysis of T cell activation regulation (2013)
  • Author(s): Saito, T.
  • Organizer: 2nd New Zealand-Japan Joint Immunology Workshop
  • Place of Presentation: Auckland, New Zealand
  • Invited
• [Presentation] Negative costimulatory microclusters inhibiting T cell-mediated inflammation (2013)
  • Author(s): Yokosuka, T.
  • Organizer: JST-CREST International Symposium ”Frontiers in Immunology and Inflammation: from Molecules to Disease”
  • Place of Presentation: 東京
• [Presentation] Spatiotemporal regulation of T cell activation and co-stimulation (2012)
  • Author(s): Saito, T.
  • Organizer: Gordon Research Conferences―Immunochemistry & Immunobiology
  • Place of Presentation: Les Diablerets, Switzerland
  • Invited
• [Presentation] CD4+T細胞の生成に必須なキナーゼの機能解析 (2012)
  • Author(s): 石川絵里
  • Organizer: Kyoto T Cell Conference
  • Place of Presentation: 京都
• [Presentation] CRTAM陽性CD4+T細胞の生体内における機能 (2012)
  • Author(s): 竹内新
  • Organizer: Kyoto T Cell Conference
  • Place of Presentation: 京都
• [Presentation] 転写因子Bach2はナイーブT細胞の形質を維持する機能を持つ (2012)
  • Author(s): 九十九伸一
  • Organizer: Kyoto T Cell Conference
  • Place of Presentation: 京都
• [Presentation] Essential role for intra- and intermolecular SH3-GUK interactions of CARMA1 in antigen receptor-induced NF-κB and JNK activation (2012)
  • Author(s): Hara, H.
  • Organizer: 第41回日本免疫学会総会学術集会
  • Place of Presentation: 神戸
• [Presentation] Oral administration of particulate beta-glucan induces IL-10-producing regulatory CD4+ T cells and protects mice from experimental colitis (2012)
  • Author(s): Yan, H.
  • Organizer: 第41回日本免疫学会総会学術集会
  • Place of Presentation: 神戸
• [Presentation] Membrane-bound human stem cell factor-expressing humanized mice support human myeloid development in vivo (2012)
  • Author(s): Takagi, S.
  • Organizer: 第41回日本免疫学会総会学術集会
  • Place of Presentation: 神戸
• [Presentation] Transport of misfolded ER proteins to the cell surface by MHC class II molecules (2012)
  • Author(s): Jiang, Y.
  • Organizer: 第41回日本免疫学会総会学術集会
  • Place of Presentation: 神戸
• [Presentation] CRTAM expression regulates localization and differentiation of CD4+T cells in vivo (2012)
  • Author(s): Takeuchi, A.
  • Organizer: 第41回日本免疫学会総会学術集会
  • Place of Presentation: 神戸
• [Book] Fundamental Immunology, 7th ed (2012)
  • Author(s): Saito T.
  • Publisher: Lippincott Williams & Wilkins, Philadelphia
• [Remarks] CD4陽性キラーT細胞への分化機構を解明
  • URL: http://www.riken.jp/pr/press/2015/20151221_1/
• [Remarks] 炎症や自己免疫疾患に関わる遺伝子の機能を解明
  • URL: http://www.riken.jp/pr/press/2013/20130611_1/
• [Patent(Industrial Property Rights)] 免疫細胞の制御技術 (2017)
  • Inventor(s): Takashi Saito, Takayuki Imanishi
  • Industrial Property Rights Holder: Takashi Saito, Takayuki Imanishi
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-028244
  • Filing Date: 2017