| Project/Area Number |
24240045
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioinformatics/Life informatics
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| Research Institution | Keio University |
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Principal Investigator |
OKA KOTARO 慶應義塾大学, 理工学部, 教授 (10276412)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Koji 慶應義塾大学, 理工学部, 教授 (80154540)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥45,760,000 (Direct Cost: ¥35,200,000、Indirect Cost: ¥10,560,000)
Fiscal Year 2014: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2013: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2012: ¥26,000,000 (Direct Cost: ¥20,000,000、Indirect Cost: ¥6,000,000)
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| Keywords | 生体生命情報学 / シグナル伝達 / 再生医学 / 生物物理 / 神経科学 / 細胞内Mgイオン / パーキンソン病 / 細胞内Caイオン / 興奮電位 / iPS細胞 / 螢光イメージング / 細胞死 / バイオイメージング / 蛍光プローブ / 細胞内Mgイオン動態 / ミトコンドリア / Ｍｇ動員 / GABA / リン酸化酵素 / 新規Mgプローブ / FRET / 一酸化窒素（NO） |
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| Outline of Final Research Achievements |
Although the magnesium ion (Mg2+) is one of the most abundant divalent cations in cells and known to play critical roles in many physiological processes, its mobilization is still obscure. To understand the Mg2+ function, first we developed a method for Mg2+ imaging in cellular local area using a novel fluorescent Mg2+ probe, “KMG-104-AsH”, and succeeded to visualize localized Mg2+ release in mitochondrial intermembrane space (MIS) induced by a protonophore, FCCP. Furthermore, we also investigated that nitric oxide (NO) activation of mitochondria ATP sensitive potassium channel, and found that NO induces Mg2+ release from mitochondria by mitochondrial depolarization via cGMP/PKG/mitoKATP channel pathway. Finally, we investigated the Parkinson-like cell death by the application of MPP+（1-methyl- 4-phenylpyridinium ion）to differentiated PC12 cells. Higher concentration of intracelluar Mg2+ moderated the toxic effect of MPP+ and suppressed the cell death.
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