|Budget Amount *help
¥46,670,000 (Direct Cost: ¥35,900,000、Indirect Cost: ¥10,770,000)
Fiscal Year 2015: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2014: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2013: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2012: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
|Outline of Final Research Achievements
The aim of this project is to develop an efficient and low-cost method to generate gene-manipulated rats for the analysis of brain functions, so that we can provide the research community of this country with gene-modified rats as useful animal resources. For this purpose, we established an optimum culture condition for rat ES cells, a rapid gene-manipulated method using homologous recombination, and a generation method of chimeras that efficiently differentiate to germ cells. Thus, we succeeded in stable generation of gene-manipulated rats. In addition, in order to produce rat sperm from the rat ES cells using our unique mouse-rat interspecies chimeric method, we generated a spermatogenesis-impaired mouse using the conditional recombination technique, and were able to generate rat spermary tissue in a mouse with high efficiency. Our project successfully developed an efficient technique to generate gene-modified rats.