| Project/Area Number |
24240053
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MIYAWAKI Atsushi 独立行政法人理化学研究所, 脳科学総合研究センター, チームリーダー (80251445)
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| Co-Investigator(Kenkyū-buntansha) |
HAMA Hiroshi 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (30261796)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIMOGORI Tomomi 独立行政法人理化学研究所, 脳科学総合研究センター, チームリーダー (30391981)
KUROKAWA Hiroshi 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (60631653)
SAKAUE Asako 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (90462689)
YOSHIHARA Yoshihiro 独立行政法人理化学研究所, 脳科学総合研究センター, チームリーダー (20220717)
ANDO Ryoko 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (10706170)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥46,930,000 (Direct Cost: ¥36,100,000、Indirect Cost: ¥10,830,000)
Fiscal Year 2014: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2013: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2012: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
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| Keywords | 組織透明化 / 3次元構築 / 神経回路 / 免疫組織学 / ３次元構築 / 三次元再構築 / 免疫組織化学 |
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| Outline of Final Research Achievements |
We developed a new Scale system with diverse applications on a spectrum from clearing to preservation. On the one hand, we cleared brain slices within hours; on the other hand, we achieved consecutive light and electron microscopic analysis for whole brain clearing at synaptic vesicle resolution. The new Scale solution was engineered for aging brain in mouse models of Alzheimer’s Disease (AD) and two applications, AbScale and ChemScale, used immunological and chemical labelling, respectively, for lucid visualization of amyloid plaques, neurons, and microglia in close proximity. The solution also cleared postmortem human brain tissue from AD patients to reveal novel and nascent amyloid plaque distribution patterns. Thus, our new Scale system is a simple, powerful, versatile, and reproducible clearing method for adult tissue with applications for multi-scale neural circuit analysis, high-resolution clinical pathology, and precise 3D visualization of cellular microenvironments.
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