| Budget Amount *help |
¥42,380,000 (Direct Cost: ¥32,600,000、Indirect Cost: ¥9,780,000)
Fiscal Year 2015: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2014: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2013: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2012: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
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| Outline of Final Research Achievements |
Aerobic glycolysis is the essential metabolic pathway for energy production in tumor cells. Gradient-enhanced heteronuclear multiple quantum coherence spectroscopy (HMQC), a kind of signal enhancement technique to truck 13C-MR tracer in vivo, was used to investigate tumor metabolism starting with 13C-labeled glucose. The detailed analysis of metabolic profile revealed that PKM2 enzyme plays pivotal role to control glycolytic flux. PKM2 inhibitor and activator suppressed lactate production and perhaps the metabolic flux bypassed to pentose phosphate pathway. Only the inhibition of lactate efflux increased the production of lactate, which indicates to modulate tumor metabolism.
We also investigated the glycolic metabolism in Alzheimer model mouse brain and found that small but definite amount of lactate signal present in any aged model mice. We concluded that lactate may play an essential role in hypometabolic brain in Alzheimer mouse model either as energy source or signaling molecule.
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