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Research on a micromachine for cellular aggregate manipulation without damage

Research Project

Project/Area Number 24240075
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionRitsumeikan University

Principal Investigator

KONISHI Satoshi  立命館大学, 理工学部, 教授 (50288627)

Co-Investigator(Kenkyū-buntansha) TABATA Yasuhiko  京都大学, 再生医科学研究所, 教授 (50211371)
TONOMURA Wataru  立命館大学, 理工学部, 助教 (50581493)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥39,780,000 (Direct Cost: ¥30,600,000、Indirect Cost: ¥9,180,000)
Fiscal Year 2014: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2013: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2012: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Keywords培養細胞組織 / マイクロマシン / バイオ操作 / アレイ化
Outline of Final Research Achievements

This study aimed to develop a micromachine for cellular aggregate manipulation without damage. We proposed two types of micromachines based on pinching and flow controlling, respectively. Pinching type micromachine holds and release by using micro fingers which is pneumatically driven. 560μm×800μm micro fingers could catch and release a cellular aggregate of 200μm in diameter in a 96 micro-well-plate. Surface modification of micro fingers improved a success rate in releasing. Flow controlling type micromachine utilized a space between an inner wall of a micro well and micromachine itself. It could capture and release a cellular aggregate of 250μm in diameter in a 96 micro-well-plate.
Based on the results, improvement of throughput in manipulation is ongoing for further practical manipulator.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (5 results)

All 2015 2014 2013 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results)

  • [Journal Article] Cellular aggregate capture by fluidic manipulation device highly compatible with micro-well-plates2015

    • Author(s)
      Satoshi Konishi, Yumi Teramachi, Shuhei Shimomura, Wataru Tonomura, Shuhei Tajima and Yasuhiko Tabata
    • Journal Title

      Biomedical Microdevices

      Volume: Vol. 17, Issue 3 Issue: 3

    • DOI

      10.1007/s10544-015-9953-x

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 圧力駆動バルーンアクチュエータを利用したマイクロフィンガーによる培養細胞の把持・解放操作2014

    • Author(s)
      下村周平、寺町有未、村松遥子、田島脩平、田畑泰彦、小西聡
    • Organizer
      ロボティクス・メカトロニクス講演会2014
    • Place of Presentation
      富山市総合体育館(富山県)
    • Year and Date
      2014-05-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] CELLULAR AGGREGATE CATCHER USING FLUIDIC MANIPULATION2013

    • Author(s)
      Satoshi Konishi
    • Organizer
      The 17th International Conference on Solid-State Sensors, Actuators and Microsystems
    • Place of Presentation
      Barcelona. Spain
    • Related Report
      2012 Annual Research Report
  • [Presentation] PINCHING AND RELEASING OF CELLULAR AGGREGATE BY MICROFINGERS USING PDMS PNEUMATIC BALLOON ACTUATORS

    • Author(s)
      Satoshi KONISHI, Yasuhiko TABATA
    • Organizer
      27th IEEE International Conference on Micro Electro Mechanical Systems
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] マイクロウェル内流体制御による培養細胞組織補足デバイス

    • Author(s)
      小西 聡, 田畑 泰彦
    • Organizer
      第30回 「センサ・マイクロマシンと応用システム」シンポジウム
    • Place of Presentation
      仙台国際センター(宮城県)
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-15   Modified: 2019-07-29  

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