| Project/Area Number |
24240076
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ABE Koji 信州大学, 学術研究院繊維学系, 教授 (00126658)
TOMINAGA Takashi 徳島文理大学, 神経科学研究所, 准教授 (20344046)
YUE Fengming 信州大学, 学術研究院医学系, 助教 (20532865)
IKEDA Uichi 信州大学, 学術研究院医学系, 教授 (30221063)
TERAMOTO Akira 信州大学, 学術研究院繊維学系, 准教授 (40227525)
SHIBA Yuji 信州大学, 医学部附属病院, 講師(特定雇用) (70613503)
SAITO Naoto 信州大学, 学術研究院保健学系, 教授 (80283258)
TOMOTUNE Daihachiro 信州大学, 学術研究院医学系, 助教 (80283802)
市川 比奈子 信州大学, 医学部附属病院, 講師 (50609617)
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| Co-Investigator(Renkei-kenkyūsha) |
ENDO Morinobu 信州大学, 先鋭領域研究群カーボン科学研究所, 特別特任教授 (10021015)
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| Research Collaborator |
YOSHIE Susumu
MASUDA Shoko
YOKOYAMA Tadayuki
TAKIZAWA Sakiko
NAGAI Mika
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥47,060,000 (Direct Cost: ¥36,200,000、Indirect Cost: ¥10,860,000)
Fiscal Year 2014: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2013: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2012: ¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000)
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| Keywords | 細胞・組織工学 / 再生医療 / 多能性幹細胞 / 細胞のプロファイリング / 網羅的遺伝子解析 / ヒートマップ解析 / 主成分解析 / カーボンナノチューブ / ナノファイバー / 主成分分析 |
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| Outline of Final Research Achievements |
Different pluripotent stem cell lines have characteristic differences making each of them unique, which is important in regenerative medicine. We compared the biological and molecular profile of 4 human embryonic stem (ES) cell lines, two human induced pluripotent stem (iPS) cell lines and two control cell types at four stages between undifferentiated to differentiated states, to identify individual characteristics. In the undifferentiated state, a group of genes were identified which differentiate ES cell lines from others. In the differentiated state, however, these gene clusters were not maintained and the individuality of each cell line became prominent, as disclosed by exhaustive gene expression analysis. The individual characteristics of each cell line were portrayed three-dimensionally on a globe. These developments are useful for analyzing the individual characteristics of pluripotent stem cell lines prior to their application in regenerative medicine.
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