| Budget Amount *help |
¥41,990,000 (Direct Cost: ¥32,300,000、Indirect Cost: ¥9,690,000)
Fiscal Year 2014: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2013: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2012: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
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| Outline of Final Research Achievements |
The most important issue for the study of damage-response is an establishment of method for direct analysis of somatic tissue cell. In this study, we have tried to establish novel molecular-genetic method using medaka as experimental model animal. The strategy we have employed is transgenesis-mediated mosaicism by use of efficient gene transfer vector. piggyback (PB) transposon can integrate into genome DNA efficiently when injected into fertilized eggs. We have produced PB-based BAC transfer vector which can transfer large genomic DNA region. Using the PB vector, we have succeeded to establish germ cell transformant and somatic mosaic.
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