Establishment of Molecular Genetic Method for Damage-response at Tissue Cell
| Project/Area Number |
24241018
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Osaka University |
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Principal Investigator |
TODO Takeshii 大阪大学, 医学(系)研究科(研究院), 教授 (90163948)
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| Co-Investigator(Kenkyū-buntansha) |
MASATO Kinoshita 京都大学, 大学院農学研究科, 助教 (60263125)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJIWARA Tomoko (ISHIKAWA Tomoko) 大阪大学, 大学院医学系研究科, 助教 (70402922)
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| Project Period (FY) |
2012-05-31 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥41,990,000 (Direct Cost: ¥32,300,000、Indirect Cost: ¥9,690,000)
Fiscal Year 2014: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2013: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2012: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
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| Keywords | 損傷応答 / メダカ / DNA損傷 / 突然変異生成 / 放射線 / 化学発ガン / 遺伝子発現制御 / 変異体作成 |
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| Outline of Final Research Achievements |
The most important issue for the study of damage-response is an establishment of method for direct analysis of somatic tissue cell. In this study, we have tried to establish novel molecular-genetic method using medaka as experimental model animal. The strategy we have employed is transgenesis-mediated mosaicism by use of efficient gene transfer vector. piggyback (PB) transposon can integrate into genome DNA efficiently when injected into fertilized eggs. We have produced PB-based BAC transfer vector which can transfer large genomic DNA region. Using the PB vector, we have succeeded to establish germ cell transformant and somatic mosaic.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] p53-Dependent suppression of genome instability in germ cells.2014
Author(s)
Otozai S, Ishikawa-Fujiwara T, Oda S, Kamei Y, Ryo H, Sato A, Nomura T, Mitani H, Tsujimura T, Inohara H, Todo T.
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Journal Title
Mutat Res,
Volume: 760
Pages: 24-32
DOI
Related Report
Peer Reviewed
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[Journal Article] Human papillomavirus and p53 mutations in head and neck squamous cell carcinoma among Japanese population.2014
Author(s)
Maruyama H, Yasui T, Ishikawa-Fujiwara T, Morii E, Yamamoto Y, Yoshii T, Takenaka Y, Nakahara S, Todo T, Hongyo T, Inohara H.
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Journal Title
Cancer Sci.
Volume: 105(4)
Issue: 4
Pages: 409-417
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A novel protein, CHRONO, functions as a core component of the mammalian circadian clock.2014
Author(s)
Goriki, A., Hatanaka, F., Myung J., Kim, J., Yoritaka, T., Tanoue, S., Abe, T., Kiyonari, H., Fujimoto, K., Kato, Y., Todo, T., Matsubara, A., Forger, D. and Takumi, T.
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Journal Title
PLoS Biol.
Volume: 12
Issue: 4
Pages: e1001839-e1001839
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] ATF6α/β-mediated adjustment of ER chaperone levels is essential for development of the notochord in medaka fish.2013
Author(s)
Ishikawa T, Okada T, Ishikawa-Fujiwara T, Todo T, Kamei Y, Shigenobu S, Tanaka M, Saito TL, Yoshimura J, Morishita S, Toyoda A, Sakaki Y, Taniguchi Y, Takeda S, Mori K.
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Journal Title
Mol Biol Cell
Volume: 24(9)
Issue: 9
Pages: 1387-1395
DOI
Related Report
Peer Reviewed
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