Basic study of a bionanodevice for medical application and expansion for its use.
| Project/Area Number |
24241043
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Tokyo Medical University (2013-2014)
Tokyo Institute of Technology (2012) |
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Principal Investigator |
HIROSHI handa 東京医科大学, 医学部, 教授 (80107432)
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| Co-Investigator(Kenkyū-buntansha) |
AOKI Ichio 独立行政法人放射線医学総合研究所, 分子イメージング研究センター, チームリーダー (10319519)
KAWANO Masaaki 埼玉医科大学, 医学部, 助教 (30447528)
MATSUI Masanori 埼玉医科大学, 医学部, 准教授 (50199741)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥46,670,000 (Direct Cost: ¥35,900,000、Indirect Cost: ¥10,770,000)
Fiscal Year 2014: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Fiscal Year 2013: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2012: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
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| Keywords | ポリオーマウイルス / simian virus 40 / VP1 / 自己集合化 / 人工粒子 / 被覆 / 分散性 / ドラッグデリバリー / ウイルス外郭タンパク質 / ＤＤＳ用キャリア / アジュバント効果 / ワクチン製剤 / ナノデバイス / ウイルス外殻タンパク質 / DDS用キャリア |
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| Outline of Final Research Achievements |
In this project, we aimed to develop a nano-device using a virus capsid subunit for medical application. For this purpose, we applied the virus capsid subunit for a coating material of various artificial beads. We found that the virus capsid subunit derived from VP1 pentamer of simian virus 40 could coat artificial beads without limitation of particle diameter of the beads. By VP1-pentamer coating, dispersity of the naked beads was greatly improved and the coated beads were retained dispersed in the physiological buffer or in the serum. It was also confirmed that the VP1-coated artificial beads where epidermal growth factor (EGF) was immobilized on the surface of the coated beds were selectively accumulated in the EGF receptor overexpressing cells with EGF dependent manner in vitro and in vivo. These findings should be useful in improvement of dispsersity of artificial beds or retention period in the body of the beds in the use of the artificial beds for drug delivery system.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] SV40 VP1 major capsid protein in its self-assembled form allows VP1 pentamers to coat various types of artificial beads in vitro regardless of their sizes and shapes2015
Author(s)
M.-A. Kawano, K. Doi, H. Fukuda, Y. Kita, K. Imai, T. Inoue, T. Enomoto, M. Matsui, M. Hatakeyama, Y. Yamaguchi, H. Handa
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Journal Title
Biotechnol. Rep.
Volume: 5
Pages: 105-111
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Structure of the human cereblon-DDB1- lenalidomide complex reveals basis for responsiveness to thalidomide analogs2014
Author(s)
P. Chamberlain, A. Lopez-Girona, K. Miller, G. Carmel, B. Pagarigan, B. Chie-Leon, E. Rychak, L. Corral, Y. Ren, M. Wang, M. Riley, S. Delker, T. Ito, H. Ando, T. Mori, Y. Hirano, H. Handa, T. Hakoshima, T. Daniel, B. Cathers
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Journal Title
Nat. Struct. Mol. Biol.
Volume: 21
Issue: 9
Pages: 803-809
DOI
Related Report
Peer Reviewed
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[Journal Article] A trifluorinated thiazoline scaffold leading to pro-apoptotic agents targeting prohibitins2014
Author(s)
A. Perez-Perarnau, S. Preciado, C. M. Palmeri, C. Moncunill-Massaguer, D. Iglesias-Serret, D. M. Gonzalez-Girones, M. Miguel, S. Karasawa, S. Sakamoto, A. M. Cosialls, C. Rubio-Patino, J. Saura-Esteller, R. Ramón, L. Caja, I. Fabregat, G. Pons, H. Handa, F. Albericio, J. Gil, R. Lavilla
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Journal Title
Angew. Chem. Int. Ed.
Volume: 53
Issue: 38
Pages: 10150-10154
DOI
Related Report
Peer Reviewed
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[Journal Article] DSIF and NELF interact with Integrator to specify the correct post-transcriptional fate of snRNA genes.2014
Author(s)
Yamamoto, J., Hagiwara, Y., Chiba, K., Isobe, T., Narita, T., Handa, H. and Yamaguchi, Y.
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Journal Title
Nat. Commun.
Volume: 5
Pages: 4263-4263
Related Report
Peer Reviewed / Open Access
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[Journal Article] Magnetically promoted rapid immunoreactions using functionalized fluorescent magnetic beads: a proof of principle2014
Author(s)
S. Sakamoto, K. Omagari, Y. Kita, Y. Mochizuki, Y. Naito, S. Kawata, S. Matsuda, O. Itano, H. Jinno, H. Takeuchi, Y. Yamaguchi, Y. Kitagawa, H. Handa
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Journal Title
Clin Chem.
Volume: 60
Issue: 4
Pages: 610-620
DOI
Related Report
Peer Reviewed
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[Journal Article] Immunomodulatory agents lenalidomide and pomalidomide co-stimulate T cells by inducing degradation of T cell repressors Ikaros and Aiolos via modulation of the E3 ubiquitin ligase complex CRL4(CRBN)2014
Author(s)
A. K. Gandhi, J. Kang, C. G. Havens, T. Conklin, Y. Ning, L. Wu, T. Ito, H. Ando, M. F. Waldman, A. Thakurta, A. Klippel, H. Handa, T.O. Daniel, P. H. Schafer, R. Chopra
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Journal Title
Br. J. Haematol.
Volume: 164
Issue: 6
Pages: 811-821
DOI
Related Report
Peer Reviewed
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[Journal Article] Inhibition of protein SUMOylation by davidiin, an ellagitannin from Davidia.2014
Author(s)
Takemoto, M., Kawamura, Y., Hirohama, M., Yamaguchi, Y., Handa, H., Saitoh, H., Nakao, Y., Kawada, M., Khalid, K., Koshino, H., Kimura, K., Ito, A., and Yoshida, M.
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Journal Title
J.Antibio.
Volume: 67
Pages: 335-338
Related Report
Peer Reviewed
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[Journal Article] Viral protein-coating of magnetic nanoparticles using simian virus 40 VP12013
Author(s)
T. Enomoto, M.-A. Kawano, H. Fukuda, W. Sawada, T. Inoue, K. C. Haw, Y. Kita, S. Sakamoto, Y. Yamaguchi, T. Imai, M. Hatakeyama, S. Saito, A. Sandhu, M. Matsui, I. Aoki, H. Handa
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Journal Title
J. Biotechnol.
Volume: 167
Issue: 1
Pages: 8-15
DOI
Related Report
Peer Reviewed
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