| Project/Area Number |
24241043
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Nanomaterials/Nanobioscience
|
|---|
| Research Institution | Tokyo Medical University (2013-2014)
Tokyo Institute of Technology (2012) |
|---|
Principal Investigator |
HIROSHI handa 東京医科大学, 医学部, 教授 (80107432)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
AOKI Ichio 独立行政法人放射線医学総合研究所, 分子イメージング研究センター, チームリーダー (10319519)
KAWANO Masaaki 埼玉医科大学, 医学部, 助教 (30447528)
MATSUI Masanori 埼玉医科大学, 医学部, 准教授 (50199741)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥46,670,000 (Direct Cost: ¥35,900,000、Indirect Cost: ¥10,770,000)
Fiscal Year 2014: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Fiscal Year 2013: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2012: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
|
|---|
| Keywords | ポリオーマウイルス / simian virus 40 / VP1 / 自己集合化 / 人工粒子 / 被覆 / 分散性 / ドラッグデリバリー / ウイルス外郭タンパク質 / ＤＤＳ用キャリア / アジュバント効果 / ワクチン製剤 / ナノデバイス / ウイルス外殻タンパク質 / DDS用キャリア |
|---|
| Outline of Final Research Achievements |
In this project, we aimed to develop a nano-device using a virus capsid subunit for medical application. For this purpose, we applied the virus capsid subunit for a coating material of various artificial beads. We found that the virus capsid subunit derived from VP1 pentamer of simian virus 40 could coat artificial beads without limitation of particle diameter of the beads. By VP1-pentamer coating, dispersity of the naked beads was greatly improved and the coated beads were retained dispersed in the physiological buffer or in the serum. It was also confirmed that the VP1-coated artificial beads where epidermal growth factor (EGF) was immobilized on the surface of the coated beds were selectively accumulated in the EGF receptor overexpressing cells with EGF dependent manner in vitro and in vivo. These findings should be useful in improvement of dispsersity of artificial beds or retention period in the body of the beds in the use of the artificial beds for drug delivery system.
|
