Development of next-generation diagnostic chip and its application to clinical diagnosis
| Project/Area Number |
24245013
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Analytical chemistry
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
TOKESHI Manabu 北海道大学, 工学(系)研究科(研究院), 教授 (60311437)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YUZAWA Yukio 藤田保健衛生大学, 医学部, 教授 (00191479)
AKIYAMA Shinichi 名古屋大学, 医学系研究科, 特任講師 (20500010)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
TANI Hirofumi 北海道大学, 大学院工学研究院, 准教授 (10271644)
ISHIDA Akihiko 北海道大学, 大学院工学研究院, 助教 (20312382)
|
|---|
| Project Period (FY) |
2012-05-31 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥46,540,000 (Direct Cost: ¥35,800,000、Indirect Cost: ¥10,740,000)
Fiscal Year 2014: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2013: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2012: ¥22,620,000 (Direct Cost: ¥17,400,000、Indirect Cost: ¥5,220,000)
|
|---|
| Keywords | チップ分析 / 免疫分析 |
|---|
| Outline of Final Research Achievements |
We developed a diagnostic chip with a five biomarker panel for diabetic nephropathy. The biomarker panel of MCP-1, L-FABP, Angiotensinogen, CTGF, and Collagen IV was adopted for diabetic nephropathy. With standard and patient samples, we evaluated the performance of the panel diagnostic chip. For the detection of biomarkers, we confirmed the chip provides rapid analysis (total assay time of 12 min) with high sensitivity and it uses small volumes of the sample and reagent (0.5 μL each), and the obtained results correlated with that of conventional ELISA.
|
Report
(4 results)
Research Products
(48 results)
-
[Journal Article] Multimarker Approach to Risk Stratification for Long-Term Mortality in Patients on Chronic Hemodialysis2015
Author(s)
Junichi Ishii, Hiroshi Takahashi, Fumihiko Kitagawa, Atsuhiro Kuno, Ryuunosuke Okuyama, Hideki Kawai, Takashi Muramatsu, Hiroyuki Naruse, Sadako Motoyama, Shigeru Matsui, Midori Hasegawa, Toru Aoyama, Daisuke Kamoi, Hirotake Kasuga, Hideo Izawa, Yukio Ozaki, Yukio Yuzawa
-
Journal Title
Circulation Journal
Volume: 79
Issue: 3
Pages: 656-663
DOI
NAID
ISSN
1346-9843, 1347-4820
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Simultaneous Detection of Multiple Disease Markers Using Immuno-Wall Devices2015
Author(s)
Nanako Nishiwaki, Akihiko Ishida, Hirofumi Tani, Toshihiro Kasama, Yoshinobu Baba, Manabu Tokeshi
Organizer
7th International Symposium on Advanced Plasma Science and Its Applications for Nitrides and Nanomaterials/8th International Conference on Plasma-Nano Technology & Science (ISPlasma2015/IC-PLANTS2015)
Place of Presentation
名古屋大学（愛知県・名古屋市）
Year and Date
2015-03-30
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
