| Budget Amount *help |
¥45,370,000 (Direct Cost: ¥34,900,000、Indirect Cost: ¥10,470,000)
Fiscal Year 2014: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2013: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
|
|---|
| Outline of Final Research Achievements |
The objective of this research project is development of novel chemistry-based methods for selective labeling of endogenous (natural) proteins not only in purified test tube, but also under crude live cell conditions. During the research, I succeeded in developing two of new ligand-directed chemistry, distinct from the previously established ligand-directed tosyl chemistry. The first is based on acyl-imidazole functional group (LDAI) which can be widely applied to membrane-bound proteins in live cells, and the second one exploits ortho-dibromophenyl-aryl ester as a cleavable functional group (termed as LDBB) that enables rapid labeling inside live cells.
|
