Development of Organic Chemistry-based Method for Endogenous Protein Modification under Live Cells
| Project/Area Number |
24245032
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kyoto University |
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Principal Investigator |
HAMACHI Itaru 京都大学, 工学(系)研究科(研究院), 教授 (90202259)
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| Project Period (FY) |
2012-05-31 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥45,370,000 (Direct Cost: ¥34,900,000、Indirect Cost: ¥10,470,000)
Fiscal Year 2014: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2013: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Keywords | 生命化学 / 細胞系反応 / 蛋白質 / ラベル化 |
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| Outline of Final Research Achievements |
The objective of this research project is development of novel chemistry-based methods for selective labeling of endogenous (natural) proteins not only in purified test tube, but also under crude live cell conditions. During the research, I succeeded in developing two of new ligand-directed chemistry, distinct from the previously established ligand-directed tosyl chemistry. The first is based on acyl-imidazole functional group (LDAI) which can be widely applied to membrane-bound proteins in live cells, and the second one exploits ortho-dibromophenyl-aryl ester as a cleavable functional group (termed as LDBB) that enables rapid labeling inside live cells.
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Report
(4 results)
Research Products
(26 results)
