Construction of database of non-canonical structures of nucleic acids and its application for constructing artificial systems controlling biological reactions
| Project/Area Number |
24245033
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Chemistry related to living body
|
|---|
| Research Institution | Konan University |
|---|
Principal Investigator |
SUGIMOTO NAOKI 甲南大学, 先端生命工学研究所, 教授 (60206430)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ENDOH Tamaki 甲南大学, 先端生命工学研究所, 講師 (90550236)
TATEISHI Hisae 甲南大学, 先端生命工学研究所, 助教 (20593495)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2014: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2013: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2012: ¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
|
|---|
| Keywords | 核酸構造 / 安定化エネルギー / 相互作用エネルギー / 分子クラウディング / 化学環境 / 四重鎖構造 / 転写反応 / 翻訳反応 / 溶液内化学環境 / ヘアピンRNA |
|---|
| Outline of Final Research Achievements |
We quantitatively analyzed biophysical parameters of nucleic acids. To obtain the biophysical parameters, we constructed experimental systems considering molecular crowding conditions, which mimic intracellular molecular environment. We investigated effects of the biophysical parameters for non-canonical nucleic acid structures on gene expression. In addition, based on the chemical properties of the non-canonical structures, we designed artificial molecules that affect formation on the structures and their stabilities. We also demonstrated that transcription and translation reactions could be controlled by using the artificial molecules and systems. The results obtained in this study will provide new insights of biological significance of the non-canonical nucleic acid structures and novel technologies to regulate their functions in cells.
|
Report
(4 results)
Research Products
(179 results)
