| Budget Amount *help |
¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2014: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2013: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2012: ¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
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| Outline of Final Research Achievements |
We quantitatively analyzed biophysical parameters of nucleic acids. To obtain the biophysical parameters, we constructed experimental systems considering molecular crowding conditions, which mimic intracellular molecular environment. We investigated effects of the biophysical parameters for non-canonical nucleic acid structures on gene expression. In addition, based on the chemical properties of the non-canonical structures, we designed artificial molecules that affect formation on the structures and their stabilities. We also demonstrated that transcription and translation reactions could be controlled by using the artificial molecules and systems. The results obtained in this study will provide new insights of biological significance of the non-canonical nucleic acid structures and novel technologies to regulate their functions in cells.
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