| Budget Amount *help |
¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000)
Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2014: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2013: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
Fiscal Year 2012: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
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| Outline of Final Research Achievements |
We have developed a biological immunoassay technique utilizing magnetic markers and highly sensitive magnetic sensors. First, we quantitatively characterized the properties of magnetic markers. These results can be used to find markers suitable for bio-application. Next, we developed a liquid-phase detection method of biological targets, where bound and free markers are magnetically distinguished by using Brownian relaxation. Therefore, we can omit a time consuming washing process called bound/free separation. We demonstrated highly sensitive detection of biological targets using this technique. Finally, we developed a magnetic-particles-imaging system for the application to in-vivo diagnosis. Magnetic particles, that were placed 50 mm below the sensors, were successfully detected with high sensitivity and spatial resolution. These results indicated the usefulness of the present systems.
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