| Project/Area Number |
24246089
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Tohoku University |
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Principal Investigator |
TATSUO Omura 東北大学, 未来科学技術共同研究センター, 教授 (30111248)
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| Co-Investigator(Kenkyū-buntansha) |
HARADA Hideki 東北大学, 大学院工学研究科, 教授 (70134971)
SANO Daisuke 北海道大学, 大学院工学研究院, 准教授 (80550368)
WATANABE Toru 山形大学, 農学部, 准教授 (10302192)
岡部 聡 北海道大学, 工学(系)研究科(研究院), 教授 (10253816)
真砂 佳史 東北大学, 工学(系)研究科(研究院), 助教 (50507895)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥46,540,000 (Direct Cost: ¥35,800,000、Indirect Cost: ¥10,740,000)
Fiscal Year 2014: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2013: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2012: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
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| Keywords | ノロウイルス / 水環境中動態 / 酸化損傷検出 / 養殖カキ / 遺伝子型 / 外殻タンパク質 / 損傷 / 水環境 / 酸化損傷 / 遺伝子損傷 / カルボニル基 |
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| Outline of Final Research Achievements |
In this study, we confirmed that the oxidative stress marker detection of viral capsid protein can be applied to the infectivity evaluation of human noroviruses in water environments when 4-log reduction of infectious titer is expected. Furthermore, a novel methodology to recover intact viral genomic RNA from environmental samples was newly established, in which only the target viral genomic RNA can be specifically recovered. In the investigation of cultivated oysters about norovirus contamination, we applied next generation sequencing technology to identify norovirus genotypes contaminatinng cultivated oysters, and found that not only predominant genotypes but laos minor strains can be detected.
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