Analysis of behavior and neural circuits with optogenetics

• Project/Area Number: 24247001
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Nagoya University
• Principal Investigator: MORI Ikue 名古屋大学, 理学(系)研究科(研究院), 教授 (90219999)
• Co-Investigator(Renkei-kenkyūsha): TSUKADA Yuki 名古屋大学, 大学院理学研究科, 助教 (80580000) ; NAKANO Shunji 名古屋大学, 大学院理学研究科, 助教 (60608529)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed(Fiscal Year 2015)
• Budget Amount: ¥41,470,000 (Direct Cost : ¥31,900,000、Indirect Cost : ¥9,570,000); Fiscal Year 2014 : ¥13,390,000 (Direct Cost : ¥10,300,000、Indirect Cost : ¥3,090,000); Fiscal Year 2013 : ¥13,260,000 (Direct Cost : ¥10,200,000、Indirect Cost : ¥3,060,000); Fiscal Year 2012 : ¥14,820,000 (Direct Cost : ¥11,400,000、Indirect Cost : ¥3,420,000)
• Keywords: 感覚神経細胞 / 記憶・学習 / 温度走性 / Cエレガンス / 神経回路

Outline of Final Research Achievements

Using thermotaxis behavior of C. elegans as a model system, we analyzed the relationship between behavioral regulation and neural activities in the neural circuit responsible for thermotaxis. We introduced and evaluated newly developed optogenetic tools to elucidate the mechanism through which a behavior is regulated in the circuit. We constructed reliable assay systems that enable to monitor neural activity and behavior. Our results show the relationship between AFD thermosensory neural activity and behavior, and shed light onto the function of stochastic activity of interneurons AIY and RIA.

Research Products

• [Journal Article] The Importance of cGMP Signaling in Sensory Cilia for Body Size Regulation in Caenorhabditis elegans. (2016)
  • Author(s): Fujiwara, M., Hino, T., Miyamoto, R., Inada, H., Mori, I., Koga, M., Miyahara, K., Ohshima, Y., Ishihara, T.
  • Journal Title: Genetics Volume : 201 Pages : 1497-1510
  • DOI: 10.1534/genetics.115.177543
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Single-Cell memory regulates a neural circuit for sensory behavior. (2016)
  • Author(s): Kobayashi, K., Nakano,S., Amano, M., Tsuboi, D., Nishioka, T., Ikeda, S., Yokoyama, G., Kaibuchi, K., Mori,I.
  • Journal Title: Cell Rep. Volume : 14 Pages : 11-21
  • DOI: 10.1016/j.celrep.2015.11.064
  • Peer Reviewed / Open Access
• [Journal Article] Reconstruction of spatial thermal gradient encoded in thermosensory neuron AFD in Caenorhabditis elegans (2016)
  • Author(s): Tsukada, Y., Yamao, M., Honda, N., Shimowada, T., Ohnishi, N., Kuhara, A., Ishii, S., Mori, I.
  • Journal Title: J. Neurosci Volume : 36 Pages : 2571-2581
  • DOI: 10.1523/jneurosci.2837-15.2016
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Optically Detected Magnetic Resonance of Nanodiamonds In Vivo; Implementation of Selective Imaging and Fast Sampling. (2015)
  • Author(s): Yoshinari, Y., Mori, S., Igarashi, R., Sugi, T., Yokota, H., Ikeda, K., Sumiya, H., Mori, I, Tochio, H., *Harada, Y. and Shirakawa, M.
  • Journal Title: J Nanosci Nanotechnol. Volume : 15 Pages : 1014-1021
  • DOI: 10.1166/jnn.2015.9739
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Molecular biology of thermosensory transduction in C. elegans. (2015)
  • Author(s): Aoki, I. and Mori, I.
  • Journal Title: Curr Opin Neurobiol. Volume : 34 Pages : 117-124
  • DOI: 10.1016/j.conb.2015.03.011
  • Peer Reviewed / Open Access
• [Journal Article] A glial K+/Cl－ cotransporter modifies temperature-evoked dynamics in Caenorhabditis elegans sensory neurons. (2015)
  • Author(s): Yoshida, A., Nakano, S., Suzuki, T., Ihara, K., Higashiyama, T., Mori, I.
  • Journal Title: Genes Brain Behav. Volume : 15 Pages : 429-440
  • DOI: 10.1111/gbb.12260
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Japanese studies on neural circuits and behavior of Caenorhabditis elegans. (2013)
  • Author(s): Sasakura, H., Tsukada, Y., Takagi, S. & Mori, I.
  • Journal Title: Front. Neural Circuits Volume : 7 Pages : 187-187
  • DOI: 10.3389/fncir.2013.00187
  • Peer Reviewed
• [Journal Article] 線虫C. elegansにおけるモノアミンによる神経制御 (2013)
  • Author(s): 笹倉寛之、森郁恵
  • Journal Title: 生体の科学 Volume : 64 Pages : 354-359
  • Peer Reviewed
• [Journal Article] dnc-1/dynactin 1 knockdown disrupts transport of autophagosomes and induces motor neuron degeneration (2013)
  • Author(s): Ikenaka K
  • Journal Title: PLoS One Volume : 8
  • DOI: 10.1371/journal.pone.0054511
  • Peer Reviewed
• [Journal Article] Behavioral plasticity, learning, and memory in C. elegans. (2013)
  • Author(s): Sasakura H, Mori I.
  • Journal Title: Curr Opin Neurobiol. Volume : 23
  • DOI: 10.1016/j.conb.2012.09.005
  • Peer Reviewed
• [Journal Article] Human myo-inositol monophosphatase 2 rescues the nematode thermotaxis mutant ttx-7 more efficiently than IMPA1: functional and evolutionary considerations of the two mammalian myo-inositol monophosphatase genes. (2013)
  • Author(s): Ohnishi, T., Tanizawa, Y., Watanabe, A., Nakamura, T., Ohba, H., Hirata, H., Kaneda, C., Iwayama, Y., Arimoto, T., Watanabe, K., Mori, I., Yoshikawa, T.
  • Journal Title: J Neurochem. Volume : 124 Pages : 685-694
  • DOI: 10.1111/jnc.12112
  • Peer Reviewed
• [Journal Article] Real-time background-free selective imaging of fluorescent nanodiamonds in vivo (2012)
  • Author(s): Ryuji Igarashi, Yohsake Yoshinari, Riroaki Yokoia, Takuma Sugi, Fuminori Sugihara, Kazuhiro Ikeda, Hiroshi Sumiya, Shigenori Tsuji, Ikme Mori, Hidehito Tochio, Yoshie Rarada, Masahiro Shirakawa.
  • Journal Title: Nano Lett Volume : 12 Pages : 5726-32
  • DOI: 10.1021/nl302979d
  • Peer Reviewed
• [Journal Article] 線虫を用いたオプトジェネティクス研究 (2012)
  • Author(s): 塚田祐基、森郁恵
  • Journal Title: 実験医学 Volume : 30 Pages : 2590-2591
• [Journal Article] Phosphatidylinositol signaling regulated by myo-inositol monophosphatase is essential for synaptic polarity in Caenorhabditis elegans (2012)
  • Author(s): Kimata T., Tanizawa Y., Can Y., Ikeda S., Kuhara A., Mori I.
  • Journal Title: Genetics Volume : 191, 2 Pages : 509-521
  • DOI: 10.1534/genetics.111.137844
  • Peer Reviewed
• [Journal Article] A novel and conserved protein AHO-3 is required for thermotactic plasticity associated with feeding states in Caenorhabditis elegans. (2012)
  • Author(s): Nishio, N., Mohri-Shiomi, A., Nishida, Y., Hiramatsu, N., Kodama-Namba, E., Kimura, K.D., Kuhara, A., and Mori, I.
  • Journal Title: Genes Cells Volume : 17 Pages : 365-386
  • DOI: 10.1111/j.1365-2443.2012.01594.x
  • Peer Reviewed
• [Presentation] Molecular, cellular, and circuit mechanisms underlying learning, memory and decision making (2016)
  • Author(s): 森 郁恵
  • Organizer: 新学術領域研究「神経細胞の多様性と大脳新皮質の構築」終了国際シンポジウム
  • Place of Presentation: 東京大学小柴ホール, 東京都
  • Year and Date: 2016-02-11
  • Int'l Joint Research
• [Presentation] 線虫C.elegansをモデル系としたイネキシンの機能解析 (2015)
  • Author(s): 塚本聡美, 延命大士, 西尾奈々, 笹倉寛之, 赤坂茉莉, 森 郁恵
  • Organizer: 第38回日本分子生物学会年会ワークショップ
  • Place of Presentation: 神戸国際会議場および神戸国際展示場, 神戸市
  • Year and Date: 2015-12-02
• [Presentation] 線虫温度走性における情報コードの再構築 (2015)
  • Author(s): 塚田祐基, 本田直樹, 山尾将隆, 石井 信, 森 郁恵
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場および神戸国際展示場, 神戸市
  • Year and Date: 2015-07-28
• [Presentation] 線虫C.elegansにおける記憶の更新に関する分子および神経回路の解析 (2015)
  • Author(s): 青木一郎, 井原邦夫, 森 郁恵
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場および神戸国際展示場, 神戸市
  • Year and Date: 2015-07-28
• [Presentation] 感覚情報の時間統合を担う分子神経基盤 (2015)
  • Author(s): 中野俊詩, Giles C. Andrew, 鈴木孝征, 佐野文菜, 東山哲也, 森 郁恵
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場および神戸国際展示場, 神戸市
  • Year and Date: 2015-07-28
• [Presentation] The C. elegans MAST Kinase Acts through Stomatin to Regulate Thermotaxis Behavior. (2015)
  • Author(s): Nakano S, A. C. Giles, Ikeda M, Suzuki T, Sano A, Higashiyama T, Mori I.
  • Organizer: C.elegans 20th International Meeting2015
  • Place of Presentation: University of California, LA, アメリカ合衆国
  • Year and Date: 2015-06-24
  • Int'l Joint Research
• [Presentation] Thermal memory and behavioral regulation revealed by calcium imaging of the cultured neurons and neural circuits. (2015)
  • Author(s): Kobayashi K, Nakano S, Amano M, Tsuboi D, Nishioka T, Ikeda S, Yokoyama G, Kaibuchi K, Mori I.
  • Organizer: C.elegans 20th International Meeting2015
  • Place of Presentation: University of California, LA, アメリカ合衆国
  • Year and Date: 2015-06-24
  • Int'l Joint Research
• [Presentation] INX-4/Innexin in AFD sensory neurons is required to regulate thermotaxis behavior in C. elegans. (2015)
  • Author(s): Tsukamoto S, Emmei T, Nishio N, Sasakura H, Akasaka M, Mori I.
  • Organizer: C.elegans 20th International Meeting2015
  • Place of Presentation: University of California, LA, アメリカ合衆国
  • Year and Date: 2015-06-24
• [Presentation] 記憶情報探索行動の分子および神経回路基盤の解明 (2014)
  • Author(s): 森 郁恵
  • Organizer: 新学術領域研究「神経細胞の多様性と大脳新皮質の構築」領域班会議
  • Place of Presentation: 京都大学芝蘭会館
  • Year and Date: 2014-12-19
• [Presentation] K+/Cl- Cotransporter KCC-3 functions in glia- like cells and regulates in thermotaxis behavior in C.elgans. (2014)
  • Author(s): Yoshida A, Nakano S, Suzuki T, Higashiyama T, Ihara K, Mori I.
  • Organizer: 第45回生理学研究所 国際シンポジウム
  • Place of Presentation: 生理学研究所, 岡崎市
  • Year and Date: 2014-11-26
  • Int'l Joint Research
• [Presentation] Roles of BK Potassium Channels in Memory Updating in C.elegans. (2014)
  • Author(s): Aoki I, Ihara K, Mori I.
  • Organizer: 第45回生理学研究所 国際シンポジウム
  • Place of Presentation: 生理学研究所, 岡崎市
  • Year and Date: 2014-11-26
  • Int'l Joint Research
• [Presentation] 線虫神経回路における意思決定、学習と記憶のデコーディング (2014)
  • Author(s): 森 郁恵
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜, 横浜市
  • Year and Date: 2014-09-11
  • Invited
• [Presentation] 線虫C.elegansにおける記憶の更新に関する分子および神経回路の解析 (2014)
  • Author(s): 青木一郎, 井原邦夫, 森郁恵
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜, 横浜市
  • Year and Date: 2014-09-11
• [Presentation] Unveiling principle of neural circuits underlying learning, memory and decision making. (2014)
  • Author(s): Ikue Mori
  • Organizer: The 11th International Congress of Neuroethology and the 36th Annual Meeting of the Japanese Society for Comparative Physiology and Biochemistry
  • Place of Presentation: 札幌コンベンションセンター, 札幌市
  • Year and Date: 2014-07-28
  • Int'l Joint Research / Invited
• [Presentation] System identification and reconstruction of sensory neural activity during thermotaxis behavior in C.elegans. (2014)
  • Author(s): Tsukada Y, Honda N, Yamada M, Murase A, Shimowada T, Ohnishi N, Kuhara I, Ishii S, Mori I.
  • Organizer: The 11th International Congress of Neuroethology and the 36th Annual Meeting of the Japanese Society for Comparative Physiology and Biochemistry
  • Place of Presentation: 札幌コンベンションセンター, 札幌市
  • Year and Date: 2014-07-28
  • Int'l Joint Research
• [Presentation] Memory updating in Caenorhabditis elegans. (2014)
  • Author(s): Aoki I, Ihara K, Mori I.
  • Organizer: The 11th International Congress of Neuroethology and the 36th Annual Meeting of the Japanese Society for Comparative Physiology and Biochemistry
  • Place of Presentation: 札幌コンベンションセンター, 札幌市
  • Year and Date: 2014-07-28
  • Int'l Joint Research
• [Presentation] The roles of biogenic amines on feeding state-dependent thermotactic behavior in C.elegans. (2014)
  • Author(s): Tsukamoto S, Nakano S, Mori I.
  • Organizer: CeNeuro2014 C.elegans Topic meeting: Neural development, synaptic function & behavior
  • Place of Presentation: ウィスコンシン大学マディソン校, アメリカ合衆国
  • Year and Date: 2014-07-07
  • Int'l Joint Research
• [Presentation] K+/Cl- Cotransporter KCC-3 regulates thermotaxis behavior in C.elegans. (2014)
  • Author(s): Yoshida A, Nakano S, Suzuki T, Higashiyama T, Ihara K, Mori I.
  • Organizer: CeNeuro2014 C.elegans Topic meeting: Neural development, synaptic function & behavior
  • Place of Presentation: ウィスコンシン大学マディソン校, アメリカ合衆国
  • Year and Date: 2014-07-07
  • Int'l Joint Research
• [Presentation] Differing Levels of MAST Kinase Activity Can Code Two Opposing Behavioral Drives During C.elegans Thermotaxis. (2014)
  • Author(s): Nakano S, de Ridder I, Suzuki T, Higashiyama T, Mori I.
  • Organizer: Differing Levels of MAST Kinase Activity Can Code Two Opposing Behavioral Drives During C.elegans Thermotaxis.
  • Place of Presentation: ウィスコンシン大学マディソン校, アメリカ合衆国
  • Year and Date: 2014-07-07
  • Int'l Joint Research
• [Presentation] Decoding neural circuits underlying learning, memory and decision-making (2014)
  • Author(s): Ikue Mori
  • Organizer: CSHL Asia Meeting, Neural Circuit Basis of Behavior and its Disorders
  • Place of Presentation: Suzhou Dushu Lake Conference Center, 蘇州, 中華人民共和国
  • Year and Date: 2014-05-12
  • Int'l Joint Research / Invited
• [Presentation] Molecular dissection of neural coding underlying behavior. (2013)
  • Author(s): Ikue Mori
  • Organizer: OIST Workshop Gradients and Signallng: from chemotaxis to development
  • Place of Presentation: Okinawa
  • Invited
• [Presentation] 線虫C. elegansの温度受容細胞における温度記憶の解析 (2013)
  • Author(s): 小林曉吾, 森郁恵
  • Organizer: Neuro2013
  • Place of Presentation: 京都
• [Presentation] C.elegans温度受容ニューロンにおけるGタンパク共役型受容体による温度受容の制御 (2013)
  • Author(s): 笹倉寛之, 伊藤浩子, 小林曉吾, 鈴木啓太, 森郁恵
  • Organizer: Neuro2013
  • Place of Presentation: 京都
• [Presentation] Thermal memory and behavioral regulation revealed by calcium imaging of the cultured neurons and neural circuits. (2013)
  • Author(s): Kobayashi, K., & Mori, I.
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: Los Angels, USA
• [Presentation] The roles of biogenic amines on feeding state-dependent thermotactic behavior in C. elegans. (2013)
  • Author(s): Tsukamoto, S., Nakano, S. & Mori, I.
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: Los Angels, USA
• [Presentation] Identification of New Genes Involved in C.elegans Thermotaxis Behavior. (2013)
  • Author(s): Nakano, S., Jiang, T., Suzuki, T., Higashiyama, T. & Mori, I.
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: Los Angels, USA
• [Presentation] Toward the identification of behavioral strategies underlying C. elegans thermotaxis using the Multi-Worm Tracker. (2013)
  • Author(s): Giles, A. C., Tsukada, Y., Nakano, S. & Mori, I.
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: Los Angels, USA
• [Presentation] G protein coupled receptor SRTX-1 is a key component for themosensation. (2013)
  • Author(s): Sasakura, H., Ito, H., Suzuki, K., Kobayashi, K. & Mori, I.
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: Los Angels, USA
• [Presentation] Insights into the molecular mechanisms of memory rewriting in Caenorhabditis elegans. (2013)
  • Author(s): Aoki, I. & Mori, I.
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: Los Angels, USA
• [Presentation] Design of the microchip device to dissect the neural circuits based on thermotactic behavior in C. elegans. (2013)
  • Author(s): Nishida, Y., Nakajima, M., Jaehoon, J., Takeuchi, M., Kobayashi, K., Fukuda, T. & Mori, I.
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: Los Angels, USA
• [Presentation] High-speed, high-magnification tracking system for calcium imaging of neurite during free moving. (2013)
  • Author(s): Tsukada, Y., Min, C., Fei, X., Hashimito, K., & Mori, I.
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: Los Angels, USA
• [Presentation] System identification for thermosensory neuron encoding thermal environment. (2013)
  • Author(s): Tsukada, Y., Honda, N., Murase, A., Shimowada, T., Onishi, N., Kuhara, A., Ishii, S. & Mori, I.
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: Los Angels, USA
• [Presentation] Information processing of the neural circuit underlying learning and memory in C. elegans.
  • Author(s): Ikue Mori
  • Organizer: 1st Nagoya International Symposium on Neural Circuits
  • Place of Presentation: Nagoya
• [Presentation] 線虫C. elegansにおける学習と記憶を含む行動の分子神経遺伝学
  • Author(s): 森郁恵
  • Organizer: 日本遺伝学会第86回大会
  • Place of Presentation: 横浜
  • Invited
• [Presentation] 行動を規定する神経回路動態の分子神経生物学
  • Author(s): 森郁恵
  • Organizer: Neuro2013
  • Place of Presentation: 京都
  • Invited
• [Presentation] Memory Circuits
  • Author(s): Ikue Mori
  • Organizer: Mittwochskolloquium, Max Planck Institute for Developmental Biology
  • Place of Presentation: Max Planck Institute(テュービンゲン市 ドイツ）
  • Invited
• [Presentation] 線虫の温度走性神経回路における光遺伝学
  • Author(s): 森郁恵
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場（愛知県）
  • Invited
• [Presentation] オプトジェネティクスによる線虫神経回路の解析
  • Author(s): 森 郁恵
  • Organizer: 文部科学省『包括型脳科学研究推進支援ネットワーク』夏のワークショップ
  • Place of Presentation: 仙台国際センター（宮城県）
  • Invited
• [Presentation] Dissecting information processing in neural circuits
  • Author(s): Ikue Mori
  • Organizer: 5th East Asia Worm Meeting
  • Place of Presentation: 救國團劍潭青年活動中心（台北市 中華民国）
  • Invited
• [Presentation] Memory, circuits and behavior
  • Author(s): Ikue Mori
  • Organizer: CSHL Asia Conference Invertebrate Neurobiology
  • Place of Presentation: Suzhou Dushu Lake Conference Center（蘇州市 中華人民共和国）
  • Invited
• [Presentation] Memory Circuits
  • Author(s): Ikue Mori
  • Organizer: EMBL Conference Series C. elegans neurobiology conference
  • Place of Presentation: EMBL Advanced Training Centre（ハイデルベルク市 ドイツ）
  • Invited
• [Book] Thermosensory Learning in Caenorhabditis elegans. In: Invertebrate Learning and Memory (Eds: Menzel, R., Benjamin, P.) (2013)
  • Author(s): Sasakura, H. & Mori, I.
  • Total Pages: 600
  • Publisher: Academic Press
• [Book] Behavioral Analysis in Caenorhabditis elegans. In: Methods in Neuroethological Research (Eds: Ogawa, H., Oka, K.) (2013)
  • Author(s): Tsukada, Y. & Mori, I.
  • Total Pages: 175
  • Publisher: Springer
• [Book] 「線虫を用いたオプトジェネティクス研究」『オプトジェネティクス―光工学と遺伝学による行動抑制技術の最前線』 (2013)
  • Author(s): 森郁恵、塚田祐基
  • Total Pages: 324
  • Publisher: NTS出版
• [Book] ”線虫を用いたオプトジェネティクス研究”オプトジェネティクス-光工学と遺伝学による行動制御技術の最前線 (2013)
  • Author(s): 塚田祐基、森郁恵
  • Total Pages: 288
  • Publisher: NTS出版
• [Book] "Behavioral Analysis in Caenorhabditis elegans" Methods in Neuroethological Research, (2013)
  • Author(s): Tsukada, Y., Mori, I.
  • Publisher: Springer