Transcriptional regulation of Shh via chromosomal dynamics
| Project/Area Number |
24247002
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
AMANO Takanori 国立遺伝学研究所, 系統生物研究センター, 助教 (20419849)
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| Research Collaborator |
SAGAI Tomoko 国立遺伝学研究所, 系統生物研究センター, 特任研究員
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥45,500,000 (Direct Cost: ¥35,000,000、Indirect Cost: ¥10,500,000)
Fiscal Year 2014: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2013: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2012: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
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| Keywords | 遺伝子発現制御 / 染色体高次構造 / エンハンサー / 発生生物学 / シジ制御配列の進化 / Shh / マウス / シス制御配列の進化 / 遺伝学 / ゲノム / 発現制御 / 発生・分化 |
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| Outline of Final Research Achievements |
The Shh locus provides a good model to study gene regulatory by tissue-specific enhancers residing at very remote regions from the Shh promoter. Our next-generation sequencing analysis revealed dynamic chromosomal architecture around the Shh locus, which is possibly responsible for the promoter-enhancer interactions in the mouse embryo. We also demonstrated different sizes of interactome between the Shh gene and neighboring genes or genomic segments. In addition, we found a phylogenetic boundary of evolutionary conservation of a larynx Shh enhancer by genome comparison among different vertebrate species. Euteleost has evolved a novel endodermal enhancer that functionally corresponds to the larynx enhancer in terrestrial animals.
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Report
(4 results)
Research Products
(12 results)
