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Transcriptional regulation of Shh via chromosomal dynamics

Research Project

Project/Area Number 24247002
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionNational Institute of Genetics

Principal Investigator

SHIROISHI Toshihio  国立遺伝学研究所, 系統生物研究センター, 教授 (90171058)

Co-Investigator(Renkei-kenkyūsha) AMANO Takanori  国立遺伝学研究所, 系統生物研究センター, 助教 (20419849)
Research Collaborator SAGAI Tomoko  国立遺伝学研究所, 系統生物研究センター, 特任研究員
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥45,500,000 (Direct Cost: ¥35,000,000、Indirect Cost: ¥10,500,000)
Fiscal Year 2014: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2013: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2012: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Keywords遺伝子発現制御 / 染色体高次構造 / エンハンサー / 発生生物学 / シジ制御配列の進化 / Shh / マウス / シス制御配列の進化 / 遺伝学 / ゲノム / 発現制御 / 発生・分化
Outline of Final Research Achievements

The Shh locus provides a good model to study gene regulatory by tissue-specific enhancers residing at very remote regions from the Shh promoter. Our next-generation sequencing analysis revealed dynamic chromosomal architecture around the Shh locus, which is possibly responsible for the promoter-enhancer interactions in the mouse embryo. We also demonstrated different sizes of interactome between the Shh gene and neighboring genes or genomic segments. In addition, we found a phylogenetic boundary of evolutionary conservation of a larynx Shh enhancer by genome comparison among different vertebrate species. Euteleost has evolved a novel endodermal enhancer that functionally corresponds to the larynx enhancer in terrestrial animals.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (12 results)

All 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) Book (1 results)

  • [Journal Article] The Hand2 gene dosage effect in developmental defects and human congenital disorders2014

    • Author(s)
      Masaru Tamura, Takanori Amano and Toshihiko Shiroishi
    • Journal Title

      Curr. Top. Dev. Biol.

      Volume: 110 Pages: 129-152

    • DOI

      10.1016/b978-0-12-405943-6.00003-8

    • ISBN
      9780124059436
    • Related Report
      2014 Annual Research Report
  • [Journal Article] A novel regulatory element for Shh expression in the lung and gut of mouse embryos2014

    • Author(s)
      Tsukiji Nagaharu, Takanori Amano and Toshihiko Shiroishi
    • Journal Title

      Mech. Dev.

      Volume: 131 Pages: 127-136

    • DOI

      10.1016/j.mod.2013.09.003

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Calpain 1 inhibitor BDA-410 ameliorates α-klotho-deficiency phenotypes resembling human aging-related syndromes.2014

    • Author(s)
      Nabeshima Y., Washida M., Tamura M., Maeno A., Ohnishi M., Shiroishi T., Imura A., Razzaque MS., Nabeshima Y.
    • Journal Title

      Sci Rep.

      Volume: 4 Issue: 1 Pages: 1-7

    • DOI

      10.1038/srep05847

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] マウスゲノムを読み解くための新しい文法書2013

    • Author(s)
      天野孝紀
    • Journal Title

      生物の科学 遺伝

      Volume: 67巻 Pages: 141-144

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Sonic hedgehog acts cell-autonomously on muscle precursor cells to generate limb muscle diversity.2012

    • Author(s)
      Anderson C
    • Journal Title

      Genes Dev.

      Volume: 26 Issue: 18 Pages: 2103-2117

    • DOI

      10.1101/gad.187807.112

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Regulatory elements for limb-specific expression of developmental genes in the coelacanth2014

    • Author(s)
      天野孝紀、城石俊彦
    • Organizer
      日本進化学会 第16回大会
    • Place of Presentation
      大阪 (高槻現代劇場)
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Enhancer sharing and promoter insulation from enhancer activities: spatial and temporal organization of gene regulation around the mouse Shh locus2014

    • Author(s)
      Takanori Amano, Tomoko Sagai, Toshihiko Shiroishi
    • Organizer
      第47回日本発生生物学会大会
    • Place of Presentation
      名古屋 (ウインク愛知)
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Elimination of an oral epithelia-specific Shh enhancer causes an extra premolar-like tooth in mouse2014

    • Author(s)
      Tomoko Sagai, Takanori Amano, Akiteru Maeno, Yoichi Mizushina, Toshihiko Shiroishi
    • Organizer
      第47回日本発生生物学会大会
    • Place of Presentation
      名古屋 (ウインク愛知)
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Mouse Hammer toe (Hm) mutation affects a long-range Shh regulation2014

    • Author(s)
      Kousuke MourI, Tomoko Sagai, Takanori Amano, Toshihiko Shiroishi
    • Organizer
      第47回日本発生生物学会大会
    • Place of Presentation
      名古屋 (ウインク愛知)
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification of a novel regulatory element for Shh expression in the mouse lung and gut2013

    • Author(s)
      Takanori Amano, Nagaharu Tsukiji, Toshihiko Shiroishi
    • Organizer
      第46回日本発生生物学会大会(APDBN共催)
    • Place of Presentation
      松江
    • Related Report
      2013 Annual Research Report
  • [Presentation] Evolutionary divergence of an epithelial linings-specific Shh enhancer2013

    • Author(s)
      Tomoko Sagai1, Takanori Amano, Akiteru MKimura, Kiyoshi aeno, Tetsuaki Naruse
    • Organizer
      第46回日本発生生物学会大会(APDBN共催)
    • Place of Presentation
      松江
    • Related Report
      2013 Annual Research Report
  • [Book] 生物の科学 遺伝 2014年5月号2014

    • Author(s)
      天野孝紀、城石俊彦
    • Total Pages
      300
    • Publisher
      株式会社 エヌ・ティー・エス
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-15   Modified: 2019-07-29  

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