| Budget Amount *help |
¥45,500,000 (Direct Cost: ¥35,000,000、Indirect Cost: ¥10,500,000)
Fiscal Year 2014: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2013: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2012: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
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| Outline of Final Research Achievements |
The Shh locus provides a good model to study gene regulatory by tissue-specific enhancers residing at very remote regions from the Shh promoter. Our next-generation sequencing analysis revealed dynamic chromosomal architecture around the Shh locus, which is possibly responsible for the promoter-enhancer interactions in the mouse embryo. We also demonstrated different sizes of interactome between the Shh gene and neighboring genes or genomic segments. In addition, we found a phylogenetic boundary of evolutionary conservation of a larynx Shh enhancer by genome comparison among different vertebrate species. Euteleost has evolved a novel endodermal enhancer that functionally corresponds to the larynx enhancer in terrestrial animals.
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