| Budget Amount *help |
¥41,730,000 (Direct Cost: ¥32,100,000、Indirect Cost: ¥9,630,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2013: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2012: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
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| Outline of Final Research Achievements |
Using in vitro ovulation system established for medaka ovarian follicles, we investigated the mechanism by which ovulation-related genes/proteins, membrane type-2 matrix metalloproteinase (MT2-MMP) and prostaglandin E2 receptor subtype (EP4b) were induced at ovulation. Expression of both genes/proteins was under the control of LH. Our data indicated that LH induced the expression of a transcription factor nuclear progestin receptor (nPR) in the granulosa cells and that nPR, together with other transcription factors, played roles in the subsequent expression of MT2-MMP and EP4b. We next examined if a communication between granulosa cells and the oocyte in the follicles may be important for successful ovulation. Preovulatory follicles were treated with carbenoxolone, a gap-junction inhibitor, using in vitro ovulation system. We found strong inhibition of MT2-MMP expression by the treatment, indicating that ovulation would be influence by oocyte maturation event occurring in the oocyte.
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