| Budget Amount *help |
¥44,330,000 (Direct Cost: ¥34,100,000、Indirect Cost: ¥10,230,000)
Fiscal Year 2015: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2014: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2013: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2012: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
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| Outline of Final Research Achievements |
Ubiquitin serves as a signaling molecule for regulation of protein degradation and other important cellular functions. In many cases, two or more ubiquitin molecules are tandemly linked through covalent bonding to function as polyubiquitin chains. In this research project, we determined three-dimensional structures of protein complexes that play important roles in synthesis, degradation and recognition of K63-linked polyubiquitin chains (K63 chains) for DNA double strand break (DSB) responses to elucidate mechanisms for the K63 chain-mediated regulation of the DSB responses at atomic resolution. Furthermore, we revealed the structure-function relationships through structure-guided mutational analyses at the molecular and cellular levels.
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