Unraveling the formation mechanism of membrane macroscopic structures by single-molecule imaging
| Project/Area Number |
24247029
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Kyoto University |
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Principal Investigator |
KUSUMI Akihiro 京都大学, 物質-細胞統合システム拠点, 教授 (50169992)
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| Co-Investigator(Kenkyū-buntansha) |
KASAI Rinshi 京都大学, 再生医科学研究所, 助教 (20447949)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥46,930,000 (Direct Cost: ¥36,100,000、Indirect Cost: ¥10,830,000)
Fiscal Year 2014: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2013: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2012: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
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| Keywords | 細胞生物物理 / 1分子計測・操作 / メゾスコピック系 / 生体分子 / 細胞膜の動的構造 |
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| Outline of Final Research Achievements |
There exist many macroscopic structures with sizes greater than 500 nm in the plasma membrane, which perform various important functions. In this study, we were interested in two macroscopic structures, the focal adhesion and the synapse. We examined and compared their fine structures and the regulation mechanisms for the formation-disintegration of their constituent molecules, using world-leading single-molecule imaging-tracking for live cells developed by us previously. Our results indicate that these structures are not single entities of massive protein assemblies as previously assumed, but largely consist of the fluid membrane dotted by protein islands of sizes less than 100 nm in diameter, which we call archipelago architectures. The channels (fluid membrane part) between the islands were found to be compartmentalized by the actin membrane skeleton, like the bulk region of the plasma membrane.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging2013
Author(s)
Nishimura H, Ritchie K, Kasai RS, Morone N, Sugimura H, Tanaka K, Sase I, Yoshimura A, Nakano Y, Fujiwara TK, and Kusumi A
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Journal Title
J Cell Biol.
Volume: 202
Issue: 6
Pages: 967-83
DOI
Related Report
Peer Reviewed
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[Journal Article] ABCA1 dimer-monomer interconversion during HDL generation revealed by single-molecule imaging.2013
Author(s)
Nagata, K., O., Nakada, C.,, Kasai, R. S.,Kusumi, A. and Ueda, K.
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Journal Title
Proc Natl Acad Sci USA
Volume: 110
Issue: 13
Pages: 5034-5039
DOI
Related Report
Peer Reviewed
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[Journal Article] Archipelago architecture of the focal adhesion: Membrane molecules freely enter and exit from the focal adhesion zone2012
Author(s)
A. C. E. Shibata, T. K. Fujiwara, L. Chen, K. G. N. Suzuki, Y. Ishikaia, Y. L. Nemoto, Y. Miwa, Z. Kalay, R. Chadda, K. Naruse, and A. Kusumi
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Journal Title
Cytoskeleton
Volume: 69
Issue: 6
Pages: 380-392
DOI
Related Report
Peer Reviewed
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[Journal Article] Cholesterol modulates cell signaling and protein networking by specifically interacting with PDZ domain-containing scaffold proteins2012
Author(s)
R. Sheng, Y. Chen, H.Y. Gee, E. Stec, H.R. Melowic, N.R. Blatner, M.P. Tun, Y. Kim, M. Kallberg, T.K. Fujiwara, J.H. Hong, K.P. Kim, H. Lu, A. Kusumi, M.G. Lee, and W. Cho
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Journal Title
Nat. Commun.
Volume: 3
Issue: 1
Pages: 1249-1249
DOI
Related Report
Peer Reviewed
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