Analysis of potential stem cells that change their fate from differentiation to self-renewal and support the regeneration of spermatogenesis
| Project/Area Number |
24247041
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
YOSHIDA Shosei 基礎生物学研究所, 生殖細胞研究部門, 教授 (60294138)
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| Project Period (FY) |
2012-05-31 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000)
Fiscal Year 2014: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2013: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2012: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
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| Keywords | 生殖細胞 / 分化 / 脱分化 / 幹細胞 / 生殖細胞研究部門 |
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| Outline of Final Research Achievements |
Using mouse spermatogenesis, his study investigated the “potential stem cells” that largely differentiate without self-renewal under a normal condition but revert back to self-renewal during regeneration after tissue damage. The discoveries made in this study include: 1) In response to retinoic acid (RA), a differentiation-inducing signal, while GFRα1+ spermatogonia (cells supporting the steady state) remain undifferentiated, while Ngn3+ cells (those acting as potential stem cells) undergo irreversible differentiation and lose the stem cell potential. 2) This differential RA responsiveness is caused by the differential expression of RARg (a RA receptor gene) only in the Ngn3+ cells. These findings suggest a novel regulatory mechanism for the robust stem cell systems.
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Report
(4 results)
Research Products
(83 results)
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[Journal Article] An epigenetic switch is crucial for spermatogonia to exit the undifferentiated state toward a Kit-positive identity2013
Author(s)
Shirakawa, T., Yaman-Deveci, R., Tomizawa, S., Kamizato, Y., Nakajima, K., Sone, H., Sato, Y., Sharif, J., Yamashita, A., Takada-Horisawa, Y., Yoshida, S., Ura, K., Muto, M., Koseki, H., Suda, T., and Ohbo, K.
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Journal Title
Development
Volume: 140
Issue: 17
Pages: 3565-3576
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 精子幹細胞の驚異的パワー2016
Author(s)
吉田松生
Organizer
生物多様性コロキウム「生殖戦略を支える細胞たち」
Place of Presentation
京都府、京都市、京都大学理学研究科
Year and Date
2016-02-24
Related Report
Invited
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[Presentation] Useful tools to investigate spermatogonial stem cells.
Author(s)
S. Yoshida
Organizer
Mini-curso “Spermatogonial stem cells physiology, niche and biotechnological potential of these cells in vertebrates” Institute of Biological Sciences at Federal University of Minas Gerais
Place of Presentation
Institute of Biological Sciences at Federal University of Minas Gerais, Belo Horizonte, Brazil
Related Report
Invited
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