| Budget Amount *help |
¥44,330,000 (Direct Cost: ¥34,100,000、Indirect Cost: ¥10,230,000)
Fiscal Year 2014: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2013: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2012: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
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| Outline of Final Research Achievements |
Although plant mitochondria genome encodes scientifically and/or agriculturally important genes, the detailed analyses are quite limited because the transformation technique of plant mitochondrial genome is still unavailable. This project tried to change the sequence of the targeted genes of Arabidopsis mitochondrial genome by using TALEN, an artificial restriction enzyme. Because each gene-targeting needs a pair of TALEN proteins with mitochondrial localizing signals, many kinds of simultaneously dual expression vectors were made. This laborious step was drastically improved by our developing method with multisite gateway system. T1 plants introduced with these TALEN expression vectors were made and some of their mitochondrial target genes were checked, but at this time point, mutation has not yet been detected.
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