Mitochondrial genome editing by TALEN

• Project/Area Number: 24248001
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: The University of Tokyo
• Principal Investigator: TSUTSUMI NOBUHIRO 東京大学, 農学生命科学研究科, 教授 (00202185)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥44,330,000 (Direct Cost: ¥34,100,000、Indirect Cost: ¥10,230,000); Fiscal Year 2014: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000); Fiscal Year 2013: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000); Fiscal Year 2012: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
• Keywords: TALEN / ミトコンドリアゲノム編集 / talen / 分子育種 / ミトコンドリア

Outline of Final Research Achievements

Although plant mitochondria genome encodes scientifically and/or agriculturally important genes, the detailed analyses are quite limited because the transformation technique of plant mitochondrial genome is still unavailable. This project tried to change the sequence of the targeted genes of Arabidopsis mitochondrial genome by using TALEN, an artificial restriction enzyme. Because each gene-targeting needs a pair of TALEN proteins with mitochondrial localizing signals, many kinds of simultaneously dual expression vectors were made. This laborious step was drastically improved by our developing method with multisite gateway system. T1 plants introduced with these TALEN expression vectors were made and some of their mitochondrial target genes were checked, but at this time point, mutation has not yet been detected.

Research Products

• [Journal Article] Arabidopsis dynamin-related proteins, DRP2A and DRP2B, function coordinately in post-Golgi trafficking (2015)
  • Author(s): Jiahe Huang, Masaru Fujimoto, Masayuki Fujiwara, Yoichiro Fukao, Shin-ichi Arimura, Nobuhiro Tsutsumi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 1 Issue: 1 Pages: 238-244
  • DOI: 10.1016/j.bbrc.2014.11.065
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] RCN1/OsABCG5, an ATP‐binding cassette (ABC) transporter, is required for hypodermal suberization of roots in rice (Oryza sativa) (2014)
  • Author(s): Katsuhiro Shiono, Miho ANDO, Shunsaku Nishiuchi, Kazufumi Yazaki, Mikio Nakazono, et al.
  • Journal Title: The Plant Journal Volume: 80 Issue: 1 Pages: 40-51
  • DOI: 10.1111/tpj.12614
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dynamin-related proteins in plant post-Golgi traffic (2014)
  • Author(s): Fujimoto, M Tsutsumi, N
  • Journal Title: Dynamin-related proteins in plant post-Golgi traffic Volume: 5 Pages: 408-408
  • DOI: 10.3389/fpls.2014.00408
  • Peer Reviewed / Open Access
• [Journal Article] Microarray analysis of laser-microdissected tissues indicates the biosynthesis of suberin in the outer part of roots during formation of a barrier to radial oxygen loss in rice (Oryza sativa). (2014)
  • Author(s): Shiono K, Yamauchi T, Yamazaki S, Mohanty B, Imran AIM, Nagamura Y, Nishizawa NK, Tsutsumi N, Colmer TD, Nakazono M.
  • Journal Title: Journal of Experimental Botany Volume: 65 Issue: 17 Pages: 4795-4806
  • DOI: 10.1093/jxb/eru235
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Flower Bud Formation of Sacred Lotus (Nelumbo nucifera Gaertn.): A Case Study of 'Gyozankouren' Grown in a Container (2014)
  • Author(s): Ishizuna, F Tsutsumi, N
  • Journal Title: HORTSCIENCE Volume: 49 Pages: 516-518
  • Peer Reviewed
• [Journal Article] Rice alcohol dehydrogenase 1 promotes survival and has a major impact on carbohydrate metabolism in the embryo and endosperm when seeds are germinated in partially oxygenated water (2014)
  • Author(s): Takahashi, H; Greenway, H ; Matsumura, H ; Tsutsumi, N; Nakazono, M
  • Journal Title: ANNALS OF BOTANY Volume: 113 Issue: 5 Pages: 851-859
  • DOI: 10.1093/aob/mct305
  • Peer Reviewed
• [Journal Article] Strigolactone and cytokinin act antagonistically in regulating rice mesocotyl elongation in darkness (2014)
  • Author(s): Hu Z, Yamauchi T, Yang J, Jikumaru Y, Tsuchida-Mayama T, Ichikawa H, Takamune I, Nagamura Y, Tsutsumi N, Yamaguchi S, Kyozuka J, Nakazono M.
  • Journal Title: Plant Cell Physiol Volume: 55巻 Issue: 1 Pages: 30-41
  • DOI: 10.1093/pcp/pct150
  • Peer Reviewed
• [Journal Article] Distribution of cellulosic wall in the anthers of Arabidopsis during microsporogenesis (2013)
  • Author(s): Yuichi Matsuo, Shin-ichi Arimura, Nobuhiro Tsutsumi
  • Journal Title: Plant Cell Reports Volume: 32 Issue: 11 Pages: 1743-1750
  • DOI: 10.1007/s00299-013-1487-1
  • Peer Reviewed
• [Journal Article] Transcriptome Analysis of Developing Ovules in Rice Isolated by Laser Microdissection (2013)
  • Author(s): Kubo, T; Fujita, M; Takahashi, H; Nakazono, M; Tsutsumi, N; Kurata, N
  • Journal Title: PLANT AND CELL PHYSIOLOGY Volume: 54 Issue: 5 Pages: 750-765
  • DOI: 10.1093/pcp/pct029
  • NAID: 10031177053
  • Peer Reviewed