| Budget Amount *help |
¥42,510,000 (Direct Cost: ¥32,700,000、Indirect Cost: ¥9,810,000)
Fiscal Year 2016: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2015: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2013: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2012: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
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| Outline of Final Research Achievements |
Molecular genetic mechanism of F1 pollen sterility in hybrids among AA genome species of genus Oryza, especially between Oryza sativa and O. glumaepatula, were elucidated in this study. By map-based cloning approach, we revealed that S22A and S22B are the members of multigene family carrying domain unknown function, which is specifically found in Poaceae, and phylogeographical origin of causal mutation of S22B in O. barthii and O. glumaepatula gene pools. We also revealed that gene duplication and loss-of-fucntion of RNA polymerase III gene caused F1 pollen sterility in O. sativa and O. nivara hybrids, suggesting that an essential gene for life cycle like house-keeping gene is possible to be a cause of reproductive isolation.
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