Quick isolation of rice QTL by whole genome sequencing

• Project/Area Number: 24248004
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: TERAUCHI Ryohei 公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 部長 (50236981)
• Co-Investigator(Kenkyū-buntansha): ISHIURA Masahiro 名古屋大学, 遺伝子実験施設, 教授 (20132730) ; ONAI Kiyoshi 名古屋大学, 遺伝子実験施設, 教授 (00402454)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥44,850,000 (Direct Cost: ¥34,500,000、Indirect Cost: ¥10,350,000); Fiscal Year 2014: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2013: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2012: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
• Keywords: 全ゲノム解析 / QTL / イネ / 生物発光リアルタイム測定 / シロイヌナズナ / DNAマーカー / 次世代シーケンス / シーケンス / 耐病性 / 病原菌

Outline of Final Research Achievements

Aiming at efficient breeding of rice cultivars, we applied QTL-seq method (Takagi et al. Plant J. 74:174) to rice to identify quantitative trait loci (QTL) controlling important agronomic traits, resulting in successful identification of QTL for cold tolerance, heading date, and partial resistance to blast disease. We further attempted to combine QTL-seq with a high-throughput luciferase bioluminescence measurement system to identify QTL governing WRKY19 gene promoter activity in Arabidopsis, with a promising preliminary result.

Research Products

• [Journal Article] A cytochromoe P450, OsDSS1, is involved in growth and drought stress responses in rice (Oryza saliva L.) (2015)
  • Author(s): Tamiru, M., Undan, J. R., Takagi, H., Abe, A., Yoshida, K., Undan, J. Q., Natsume, S., Uemura, A., Saitoh, H., Matsumura, H., Urasaki, N., Yokota, T., Terauchi, R.
  • Journal Title: Plant Molecular Biology Volume: 88 Issue: 1-2 Pages: 85-99
  • DOI: 10.1007/s11103-015-0310-5
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] MutMap accelerates breeding of a salt-tolerant rice cultivar (2015)
  • Author(s): Takagi, H., Tamiru, M., Abe, A., Yoshida, K., Uemura, A., Yaegashi, H., Obara, T., Oikawa, K., Utsushi, H., Kanzaki, E., Mitsuoka, C., Natsume, S., Kosugi, S., Kanzaki, H., Matsumura, H., Urasaki, N., Kamoun, S., Terauchi, R.
  • Journal Title: Nature Biotechnology Volume: 33 Issue: 5 Pages: 445-449
  • DOI: 10.1038/nbt.3188
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Harvesting the promising fruits of genomics: applying genome sequencing technologies to crop breeding (2014)
  • Author(s): Varshney, R. K., Terauchi, R., McCouch, S. R.
  • Journal Title: PLoS Biology Volume: 12 Issue: 6 Pages: 1-7
  • DOI: 10.1371/journal.pbio.1001883
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The NB-LRR proteins RGA4 and RGA5 interact functionally and physically to confer disease resistance (2014)
  • Author(s): Cesari, S., Kanzaki, H., Fujiwara, T., Bernoux, M., Chalvon, V., Kawano, Y., Shimamoto, K., Dodds, P., Terauchi, R., Kroj, T.
  • Journal Title: EMBO Journal Volume: 33 Issue: 17 Pages: 1941-1959
  • DOI: 10.15252/embj.201487923
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The rice (Oryza saliva L.) LESION MIMIC RESEMBLING, which encodes an AAA-type ATPase, is implicated in defense response (2014)
  • Author(s): Fekih, R., Tamiru, M., Kanzaki, H., Abe, A., Yoshida, K., Kanzaki, E., Saitoh, H., Takagi, H., Natsume, S., Undan, J. R., Undan, J, Terauchi, R.
  • Journal Title: Molecular Genetics and Genomics Volume: 290 Issue: 2 Pages: 611-622
  • DOI: 10.1007/s00438-014-0944-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The tillering phenotype of the rice plastid terminal oxidase (PTOX) loss-of-function mutant is associated with strigolactone deficiency (2014)
  • Author(s): Tamiru,M., Abe,A., Utsushi,H., Yoshida,K., Takagi,H., Fujisaki,K., Undan,J.R., Rakshit,S., Takaichi,S., Jikumaru,Y., Yokota,T., Terry,M.J., Terauchi,R.
  • Journal Title: New Phytologist Volume: 202 Issue: 1 Pages: 116-131
  • DOI: 10.1111/nph.12630
  • Peer Reviewed
• [Journal Article] MutMap+ : Genetic mapping and mutant identification without crossing in rice (2013)
  • Author(s): Fekih, R., Takagi, H., Tamiru, M., Abe, A., Natsume, S., et al.
  • Journal Title: PLoS One Volume: 8 Issue: 7 Pages: e68529-e68529
  • DOI: 10.1371/journal.pone.0068529
  • Peer Reviewed
• [Journal Article] MutMap-Gap:whole-genome resequencing of mutant F2 progeny bulk combined with de novo assembly of gap regions identifies the rice blast resistance gne Pii (2013)
  • Author(s): Takagi,H., Uemura,A., Yaegashi,H., Tamiru, M., Abe, A., Mitsuoka,C., Utsushi,H., Natsume,S., Kanzaki,H., Matsumura,H., Saitoh,H., Yoshida,K., Cano,L.M., Kamoun,S., Terauchi,R.
  • Journal Title: New Phytologist Volume: 200 Issue: 1 Pages: 276-283
  • DOI: 10.1111/nph.12369
  • Peer Reviewed
• [Journal Article] QTL-seq: rapid mapping of quantitative trait loci in rice by whole genome resequencing of DNA from two bulked populations (2013)
  • Author(s): TAKAGI, H.
  • Journal Title: The Plant Journal Volume: 74 Issue: 1 Pages: 174-183
  • DOI: 10.1111/tpj.12105
  • Peer Reviewed
• [Presentation] いもち病菌とイネ相互作用の解析 (2013)
  • Author(s): 寺内良平
  • Organizer: 第５４回日本植物生理学会年会
  • Place of Presentation: 岡山大学
  • Invited
• [Presentation] 完全な全ゲノム配列が決定されていないイネ品種の突然変異体における迅速な原因遺伝子単離方法 (2013)
  • Author(s): 高木宏樹
  • Organizer: 第１２３回日本育種学会講演会
  • Place of Presentation: 東京農業大学
• [Presentation] 第１２３回日本育種学会講演会 (2013)
  • Author(s): 阿部陽
  • Organizer: QTL-seq法によるイネ高度穂孕み期耐冷性遺伝子座の同定
  • Place of Presentation: 東京農業大学
• [Presentation] Toward understanding Magnaporthe-rice interactions 発 表 場 所 (2012)
  • Author(s): TERAUCHI, R.
  • Organizer: 15th International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: 京都国際会議場
  • Invited
• [Presentation] Toward understanding Magnaporthe-rice interactions (2012)
  • Author(s): TERAUCHI, R.
  • Organizer: 30th New Phytologist Symposium: Immunomodulation by plant-associated organisms
  • Place of Presentation: Fallen Leaf Lake, California, USA
  • Invited
• [Presentation] 全ゲノム情報を活用したイネ－いもち病菌相互作用の解析 (2012)
  • Author(s): 寺内良平
  • Organizer: 平成２４年度植物感染生理談話会
  • Place of Presentation: 近江八幡国民休暇村
  • Invited
• [Presentation] MutMap, MutMap+ & MutMap-Gap:次世代シーケンサーを用いた遺伝子単離技術
  • Author(s): 高木宏樹, 八重樫弘樹, 植村亜衣子, 宇津志博恵, 夏目俊, 阿部陽, 寺内良平
  • Organizer: 第１２４回日本育種学会講演会
  • Place of Presentation: 鹿児島大学
  • Invited
• [Presentation] 分子間相互作用によるイネ－いもち病菌の共進化
  • Author(s): 寺内良平, 神崎洋之, 齋藤宏昌, 藤崎恒喜, 高木宏樹
  • Organizer: 第１２４回日本育種学会講演会
  • Place of Presentation: 鹿児島大学
  • Invited
• [Presentation] 植物－病原菌相互作用の集団ゲノム解析
  • Author(s): 寺内良平
  • Organizer: 日本遺伝学会第８５回大会
  • Place of Presentation: 慶応大学
  • Invited
• [Presentation] Whole genome analysis of rice-Magnaporthe interactions
  • Author(s): Terauchi, R.
  • Organizer: 10th International Congress of Plant Pathology
  • Place of Presentation: Beijing, China
  • Invited