• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Acceleration of functional genomics by virus-induced gene silencing

Research Project

Project/Area Number 24248006
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionTohoku University

Principal Investigator

Kanayama Yoshinori  東北大学, 農学研究科, 教授 (10233868)

Co-Investigator(Kenkyū-buntansha) 田尾 龍太郎  京都大学, (連合)農学研究科(研究院), 准教授 (10211997)
高橋 英樹  東北大学, (連合)農学研究科(研究院), 教授 (20197164)
Co-Investigator(Renkei-kenkyūsha) TAO Ryutaro  京都大学, 大学院農学研究科, 教授 (10211997)
Project Period (FY) 2012-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥36,530,000 (Direct Cost: ¥28,100,000、Indirect Cost: ¥8,430,000)
Fiscal Year 2015: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2014: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2013: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2012: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Keywordsサイレンシング / 園芸作物 / 果樹 / 野菜 / 花き / ウイルス / 観賞植物
Outline of Final Research Achievements

Accumulation of genome-related information is advanced in horticultural crops; however, its contribution to horticultural production is not necessarily high. One of the main reasons is that the functional genomics method to prove the function of a gene has not been established in the field of horticulture including various species. Therefore, the purpose of this study was to accelerate the identification of the function of useful genes by applying virus-induced gene silencing to horticultural crops. In this study, we analyzed the physiological role and horticultural importance of genes to be controlled in fruit trees, vegetables, and flowers. In addition, we tried to develop viral vectors applicable to various horticultural crops, and to examine the mechanism of infection related to the use of cucumber mosaic virus with potentially general versatility because of wide host range.

Report

(5 results)
  • 2016 Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (35 results)

All 2017 2016 2015 2014 2013 2012

All Journal Article (14 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 13 results,  Acknowledgement Compliant: 4 results,  Open Access: 3 results) Presentation (21 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Characterization of Flowering-related Genes and Flowering Response in Relation to Blue Light in <i>Gypsophila paniculata</i>2017

    • Author(s)
      Tomoki Shibuya, Yuki Murakawa, Koji Nishidate, Manabu Nishiyama, Yoshinori Kanayama
    • Journal Title

      The Horticulture Journal

      Volume: 86 Issue: 1 Pages: 94-104

    • DOI

      10.2503/hortj.MI-126

    • NAID

      130005312833

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Serotonin content in fresh and processed tomatoes and its accumulation during fruit development2017

    • Author(s)
      Shohei Hano, Tomoki Shibuya, Nozomi Imoto, Ayaka Ito, Shunsuke Imanishi, Hisashi Aso, Yoshinori Kanayama
    • Journal Title

      Scientia Horticulturae

      Volume: 214 Pages: 107-113

    • DOI

      10.1016/j.scienta.2016.11.009

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Expression of an extracellular ribonuclease gene increases resistance to Cucumber mosaic virus in tobacco2016

    • Author(s)
      Teppei Sugawara, Ekaterina A. Trifonova, Alexey V. Kochetov, Yoshinori Kanayama
    • Journal Title

      BMC Plant Biology

      Volume: 16 Issue: S3

    • DOI

      10.1186/s12870-016-0928-8

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Virus-induced gene silencing in various Prunus species with the Apple latent spherical virus vector2016

    • Author(s)
      Kawai, T., A. Gonoi, M. Nitta, N. Yamagishi, N. Yoshikawa and R. Tao
    • Journal Title

      Scientia Hort.

      Volume: 199 Pages: 103-113

    • DOI

      10.1016/j.scienta.2015.12.031

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic Metabolic Regulation by a Chromosome Segment from a Wild Relative During Fruit Development in a Tomato Introgression Line, IL8-32016

    • Author(s)
      Hiroki Ikeda, Tomoki Shibuya, Shunsuke Imanishi, Hisashi Aso, Manabu Nishiyama and Yoshinori Kanayama
    • Journal Title

      Plant and Cell Physiology

      Volume: 57 Issue: 6 Pages: 1257-1270

    • DOI

      10.1093/pcp/pcw075

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Ionomic Analysis of Horticultural Plants Reveals Tissue-specific Element Accumulation2015

    • Author(s)
      Tomoki Shibuya, Toshihiro Watanabe, Hiroki Ikeda, Yoshinori Kanayama
    • Journal Title

      The Horticulture Journal

      Volume: 84 Issue: 4 Pages: 305-313

    • DOI

      10.2503/hortj.MI-058

    • NAID

      130005105344

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of an uncharacterized aldo-keto reductase gene from peach and its role in abiotic stress tolerance2014

    • Author(s)
      Y. Kanayama, R. Mizutani, S. Yaguchi, A. Hojo, H. Ikeda, M. Nishiyama, K. Kanahama
    • Journal Title

      Phytochemistry

      Volume: 104 Pages: 30-36

    • DOI

      10.1016/j.phytochem.2014.04.008

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Virus-induced Gene Silencing in Apricot (<i>Prunus armeniaca</i> L.) and Japanese Apricot (<i>P. mume</i> Siebold &amp; Zucc.) with the <i>Apple Latent Spherical Virus</i> Vector System2014

    • Author(s)
      Takashi Kawai, Ayako Gonoi, Michiya Nitta, Masanori Kaido, Noriko Yamagishi, Nobuyuki Yoshikawa and Ryutaro Tao
    • Journal Title

      Journal of the Japanese Society for Horticultural Science

      Volume: 83 Issue: 1 Pages: 23-31

    • DOI

      10.2503/jjshs1.CH-091

    • NAID

      130004510811

    • ISSN
      1882-3351, 1882-336X
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of a tomato introgression line, IL8-3, with increased Brix content. .2013

    • Author(s)
      Hiroki Ikeda, Masahiro Hiraga, Kenta Shirasawa, Manabu Nishiyama, Koki Kanahama, Yoshinori Kanayama
    • Journal Title

      Scientia Horticulturae

      Volume: 153 Pages: 103-108

    • DOI

      10.1016/j.scienta.2013.02.006

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Possible role of a peach homolog of UDP-glucose dehydrogenase in fruit development2013

    • Author(s)
      Keiko Sato
    • Journal Title

      Scientia Horticulturae

      Volume: 150 Pages: 195-200

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cyclic nucleotide-gated ion channel-mediated cell death may not be critical for R gene-conferred resistance to Cucumber mosaic virus in Arabidopsis thaliana2012

    • Author(s)
      Hideki Takahashi
    • Journal Title

      Physiological and Molecular Plant Pathology

      Volume: 79 Pages: 40-48

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RCY1-Mediated Resistance to Cucumber mosaic virus Is Regulated by LRR Domain-Mediated Interaction with CMV(Y) Following Degradation of RCY12012

    • Author(s)
      Hideki Takahashi
    • Journal Title

      Molecular Plant-Microbe Interactions

      Volume: 25 Pages: 1171-1185

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of vacuolar H+-inorganic pyrophosphatase in tomato fruit development2012

    • Author(s)
      Seedahmed A. Mohammed
    • Journal Title

      Journal of Experimental Botany

      Volume: 63 Pages: 5613-5621

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analyses of early rough bark phenotype found in seedlings of a cross between the European pear ‘Bartlett’ and the Chinese pear ‘Yali’2012

    • Author(s)
      Akiko Ogata
    • Journal Title

      Scientia Horticulturae

      Volume: 148 Pages: 1-8

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Dynamic Metabolic Regulation by a Chromosome Segment from a Wild Species During Fruit Development in a Tomato Introgression Line2016

    • Author(s)
      Y. Kanayama
    • Organizer
      The 10th International Conference on Bioinformatics of Genome Regulation and Structure/System Biology
    • Place of Presentation
      Novosibirsk, Russia
    • Year and Date
      2016-08-29
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] トルコギキョウの花成および花成関連遺伝子の発現に及ぼす光質の影響2016

    • Author(s)
      高橋拓馬・渋谷知暉・西山学・金山喜則
    • Organizer
      園芸学会春季大会
    • Place of Presentation
      東京農業大学厚木キャンパス(神奈川県厚木市)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] トマトの染色体断片置換系統における発芽期の塩ストレス耐性に関する研究.2016

    • Author(s)
      菅田瑛夏・秋山明子・西山学・金山喜則
    • Organizer
      園芸学会春季大会
    • Place of Presentation
      東京農業大学厚木キャンパス(神奈川県厚木市)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] トマトにおける青色光受容体FKF1ホモログの解析2015

    • Author(s)
      渋谷知暉・西山学・金山喜則
    • Organizer
      園芸学会秋季大会
    • Place of Presentation
      徳島大学常三島キャンパス(徳島県徳島市)
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] トマトの染色体断片置換系統におけるアミノ酸代謝に関する研究2015

    • Author(s)
      渋谷知暉・池田裕樹・西山学・金山喜則
    • Organizer
      園芸学会秋季大会
    • Place of Presentation
      徳島大学常三島キャンパス(徳島県徳島市)
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] セイヨウナシの LEA14 ホモログの低温ストレス耐性における役割2015

    • Author(s)
      前田未野里・西山学・金濱耕基・金山喜則
    • Organizer
      園芸学会春季大会
    • Place of Presentation
      千葉大学
    • Year and Date
      2015-03-28 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 数種長日性切り花類の開花に及ぼす光質の影響2015

    • Author(s)
      高橋拓馬・渋谷知暉・西山学・金浜耕基・金山喜則
    • Organizer
      園芸学会春季大会
    • Place of Presentation
      千葉大学
    • Year and Date
      2015-03-28 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] トマトの染色体断片置換系統における果実の発育段階別のオミクス解析2014

    • Author(s)
      池田裕樹・今西俊介・金浜耕基・金山喜則
    • Organizer
      園芸学会秋季大会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-27 – 2014-09-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] シュッコンカスミソウの長日処理に効果的な光質と花成関連遺伝子の発現2014

    • Author(s)
      渋谷知暉・西山 学・金浜耕基・金山喜則
    • Organizer
      園芸学会東北支部
    • Place of Presentation
      弘前大学
    • Year and Date
      2014-08-27 – 2014-08-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] シュッコンカスミソウの花成誘導と関連遺伝子の発現に及ぼす光質の影響の多様性2014

    • Author(s)
      渋谷知暉・村川雄紀・西山学・金浜耕基・金山喜則
    • Organizer
      植物細胞分子生物学会
    • Place of Presentation
      岩手大学
    • Year and Date
      2014-08-21 – 2014-08-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 青色光がシュッコンカスミソウの花成関連遺伝子の発現に及ぼす影響2014

    • Author(s)
      渋谷知暉・村川雄紀・西山学・金浜耕基・金山喜則
    • Organizer
      園芸学会
    • Place of Presentation
      筑波大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] トマトにおけるポリオールとアルドース‐6‐リン酸還元酵素の解析2014

    • Author(s)
      勝又淳司・大畠健介・ 西山学・金濱耕基・金山喜則
    • Organizer
      園芸学会
    • Place of Presentation
      筑波大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] トマトの第8 染色体における染色体断片置換系統の果実成分に関する研究2013

    • Author(s)
      池田裕樹,西山学,金浜耕基,金山喜則
    • Organizer
      日本植物細胞分子生物学会
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細胞外リボヌクレアーゼを導入したタバコのキュウリモザイクウイルス耐性2013

    • Author(s)
      勝又淳司*・菅原哲平・E. A. Trifonova・A. V. Kochetov・金山喜則
    • Organizer
      園芸学会
    • Place of Presentation
      岩手大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] トマトの第8染色体における染色体断片置換系統を用いた果実の糖度に関する研究2013

    • Author(s)
      池田裕樹
    • Organizer
      植物生理学会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] トマトの染色体断片置換系統を用いた糖度QTLの単離に関する研究2013

    • Author(s)
      池田裕樹
    • Organizer
      園芸学会
    • Place of Presentation
      東京農工大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] モモのアルドーケト還元酵素PpAKR1の役割に関する研究2013

    • Author(s)
      水谷亮介・西山学・金濱耕基・金山喜則
    • Organizer
      園芸学会
    • Place of Presentation
      東京農工大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] トマト果実の発育におけるV-PPaseの役割2012

    • Author(s)
      金山喜則
    • Organizer
      園芸学会
    • Place of Presentation
      福井県立大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] olanum pennellii の第 8 染色体における有用形質に関する研究2012

    • Author(s)
      池田裕樹
    • Organizer
      園芸学会
    • Place of Presentation
      福井県立大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] An tomato introgression line with increased fruit soluble solids2012

    • Author(s)
      Hiroki Ikeda
    • Organizer
      10th International Congress on Plant Molecular Biology
    • Place of Presentation
      Jeju Island, Korea
    • Related Report
      2012 Annual Research Report
  • [Presentation] Tolerance to salt stress and blossom-end rot in an tomato introgression line, IL8-32012

    • Author(s)
      Hiroki Ikeda
    • Organizer
      10th International Congress on Plant Molecular Biology
    • Place of Presentation
      Jeju Island, Korea
    • Related Report
      2012 Annual Research Report

URL: 

Published: 2013-05-15   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi