Identification and application of genes involved in accumulation of toxic elements in rice

• Project/Area Number: 24248014
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Okayama University
• Principal Investigator: MA Jian Feng 岡山大学, その他部局等, 教授 (80260389)
• Co-Investigator(Kenkyū-buntansha): YAMAJI Naoki 岡山大学, 資源植物科学研究所, 助教授 (00444646) ; MITANI Namiki 岡山大学, 資源植物科学研究所, 助教 (40581020)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥44,850,000 (Direct Cost: ¥34,500,000、Indirect Cost: ¥10,350,000); Fiscal Year 2014: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000); Fiscal Year 2013: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000); Fiscal Year 2012: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
• Keywords: カドミウム / ヒ素 / イネ / トランスポーター / 輸送体 / 品種間差

Outline of Final Research Achievements

We identified novel genes involved in accumulation of cadmium (Cd) and arsenic (As) in rice. We found that OsNramp5 is a major transporter for Cd uptake in rice. OsNramp5 is polarly localized at the distal side of root exodermis and endodermis. Over-expression of OsHMA3, a tonoplast-localized Cd transporter, resulted in enhanced tolerance to Cd toxicity. Furthermore, we found that OsHMA2 localized at the node is involved in distribution of Cd to the grain. On the other hand, we found that OsABCC1 reduces As accumulation to the grain in rice. OsABCC1 is mainly localized to the tonoplast of phloem companion cells of the node.

Research Products

• [Journal Article] A rice ABC transporter, OsABCC1, reduces arsenic accumulation in the grain (2014)
  • Author(s): Song, W. Y., Yamaki, T., Yamaji, N., Ko, D., Jung, K. H., Fujii-Kashino, M., An, G., Martinoia, E., Lee, Y. and Ma, J. F.
  • Journal Title: Proc Natl Acad Sci USA Volume: 111 Issue: 44 Pages: 15699-15704
  • DOI: 10.1073/pnas.1414968111
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Overexpression of OsHMA3 enhances Cd tolerance and expression of Zn transporter genes in rice (2014)
  • Author(s): Sasaki, A., Yamaji , N. and Ma, J. F. 2014.
  • Journal Title: J. Exp. Bot. Volume: 65 Issue: 9 Pages: 6013-6021
  • DOI: 10.1016/j.tplants.2014.05.007
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Root and shoot transcriptome analysis of two ecotypes of Noccaea caerulescens uncovers the role of NcNramp1 in Cd hyperaccumulation. (2014)
  • Author(s): Milner, M., Mitani-Ueno, N., Yamaji, N., Yokosho, K., Craft, E., Fei, Z., Ebbs, S., Zambrano, M., Ma, J. F*. Kochian, L
  • Journal Title: Plant J Volume: 78 Issue: 3 Pages: 398-410
  • DOI: 10.1111/tpj.12480
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Preferential delivery of Zn to developing tissues in rice is mediated by a P-type ATPases, OsHMA2 (2013)
  • Author(s): Yamaji, N., Xia, J. X., Mitani-Ueno, N., Yokosho, K. and Ma, J. F.
  • Journal Title: Plant Physiol. Volume: 162 Issue: 2 Pages: 927-939
  • DOI: 10.1104/pp.113.216564
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Nramp5 is a major transporter responsible for manganese and cadmium uptake in rice (2012)
  • Author(s): Sasaki, A., Yamaji, N., Yokosho, K. and Ma, J. F.
  • Journal Title: Plant Cell Volume: 24 Pages: 2155-2167
  • Peer Reviewed
• [Journal Article] Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation (2012)
  • Author(s): Milner, M. J., Craft, E., Yamaji, N., Koyama, E., Ma, J. F. and Kochian, L. V.
  • Journal Title: New Phytol. Volume: 195 Pages: 113-123
  • Peer Reviewed
• [Presentation] Transporters involved in cadmium accumulation in plants (2014)
  • Author(s): Ma, J. F.
  • Organizer: Development of “Knowledge about Agriculture” through the International Collaboration between the University of Tokyo, Japan and the Southwest University, China
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2014-09-12 – 2014-09-13
  • Invited
• [Presentation] Physiological and genetic characterization of two rice cultivars differing in Cd accumulation (2014)
  • Author(s): 鄧 鋒林・福岡修一・正村純彦・安藤 露・馬 建鋒
  • Organizer: 日本土壌肥料学会年会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] Transporters involved in Cd accumulation in rice (2013)
  • Author(s): Ma, J. F.
  • Organizer: 4th Annual Conference on Cost Action FA 0905, Mineral Improved Crop Production for Healthy Food and Feed.
  • Place of Presentation: オスロ
  • Invited
• [Presentation] OsNramp5 is a major transporter for uptake of Mn and Cd in rice (2013)
  • Author(s): Sasaki, A., Yamaji, N., Yokosho, K. and Ma, J. F.
  • Organizer: XVII International Plant Nutrition Colloquium 2013, Plant Nutrition for Nutrient and Food Security
  • Place of Presentation: イスタンブール
• [Presentation] 植物のミネラルトランスポーター (2012)
  • Author(s): 馬 建鋒
  • Organizer: 第53回日本生化学会，中国四国支部例会
  • Place of Presentation: 岡山
• [Presentation] Plant transporters for transition metals (2012)
  • Author(s): 馬 建鋒
  • Organizer: 第12回日本蛋白質科学会年会
  • Place of Presentation: 名古屋
• [Presentation] イネOsHMA3過剰発現体の更なる解析 (2012)
  • Author(s): 馬 建鋒
  • Organizer: 日本土壌肥料学会年会
  • Place of Presentation: 鳥取
• [Presentation] イネのOsHMA2による亜鉛およびカドミウムの選択的分配機構 (2012)
  • Author(s): 山地直樹・夏 継星・馬 建鋒
  • Organizer: 日本土壌肥料学会年会
  • Place of Presentation: 鳥取
• [Remarks]
  • URL: http://www.rib.okayama-u.ac.jp/plant.stress/index-j.html
• [Remarks] www.rib.okayama-u.ac.jp/plant.stress/index.html
• [Remarks] www.rib.okayama-u.ac.jp/plant.stress/index.html